Phytochemical screening, total phenolic content and antioxidant activities of Marrubium vulgare L. collected in three development stages


  • Aya KHOUCHLAA National Agency of Medicinal and Aromatic Plants, Laboratory of Biochemistry, BP 159, Principal, Taounate, 34000 (MA)
  • Abdessadek RAHIMI National Agency of Medicinal and Aromatic Plants, Laboratory of Biochemistry, BP 159, Principal, Taounate, 34000; University Mohammed First (UMP), Faculty of Sciences Oujda (FSO), Laboratory of Applied Chemistry and Environment (LCAE), Oujda 6000 (MA)
  • Mostafa MIMOUNI University Mohammed First (UMP), Faculty of Sciences Oujda (FSO), Laboratory of Applied Chemistry and Environment (LCAE), Oujda 6000 (MA)
  • Chaimae RAIS National Agency of Medicinal and Aromatic Plants, Laboratory of Botany, BP 159, Principal, Taounate, 34000 (MA)
  • Hamid KHAMAR University Mohammed V in Rabat, Scientific Institute, Department of Botany and Plant Ecology, B. P. 703, Rabat 10106 (MA)
  • Fatna ZAAKOUR Chouaib Doukkali University, Department of Agricultural and Environmental Engineering, Laboratory of Sustainable Agriculture Management, Higher School of Technology Sidi Bennour, Av. des Facultés, 24123 El Haouzia, El Jadida (MA)



antioxidant activity, M. vulgare, phenols content, screening


The change of phenolic compounds and antioxidant activities of plants extracts are widely attributed to numerous conditions and parameters viz. geographical conditions, type of extraction, and type of extract. Thus, this study was the first one conducted to evaluate the phenolic composition, the content of total polyphenols, flavonoids, and proanthocyanidins, and the antioxidant activities of methanolic extract of M. vulgare aerial part collected in three development stages (before flowering, during flowering, and after flowering periods). Phytochemical screening was carried out using standard methods of precipitation and coloration reactions. The total polyphenol, flavonoid, and proanthocyanidins contents were determined using the Folin-Ciocalteu method, aluminum chloride colorimetric method, and vanillin method, respectively. The antioxidant activity was assessed by two methods: DPPH and ABTS scavenging activity. Preliminarily phytochemical screening revealed the presence of saponins, essential oil, cardiac glucosides, sterols and triterpenes, and the absence of alkaloids. In addition, tannins were absence in March and present in May and July. The total phenol content of M. vulgare extracts showed a highest flavonoid and proanthocyanidin contents during flowering period (1.644 mg QE.g-1 extract, and 0.195 mg CE.g-1 extract, respectively) while polyphenol content was highest before flowering period (0.043 mg GAE.g-1 extract). Afterward, M. vulgare methanolic extract exhibited a potent antioxidant activity during flowering period with an IC50 value of 2.23 From the current study, this plant contains natural antioxidant substances which can be used as antioxidant agents to treat several diseases.


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How to Cite

KHOUCHLAA, A., RAHIMI, A., MIMOUNI, M., RAIS, C., KHAMAR, H., & ZAAKOUR, F. (2023). Phytochemical screening, total phenolic content and antioxidant activities of Marrubium vulgare L. collected in three development stages. Notulae Scientia Biologicae, 15(3), 11615.



Research articles
DOI: 10.55779/nsb15311615

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