Phytochemical studies and in vitro evaluation of the antioxidant activity of some medicinal and aromatic plants from Morocco
Keywords:antioxidant activity, medicinal plants, Morocco, oxidative stress, phenolic compounds, radical scavenging
The present work was carried out to evaluate the phenolic compounds and the antioxidant activities of some solvent extract (methanol, hydroethanol and aqueous) of several Moroccan medicinal plants known for their high antioxidant properties. The extracts were obtained by sonication, then, the total phenolics and flavonoids compounds were determined using Folin-Ciocalteu and Aluminium chloride. Afterwards, the Total Antioxidant Capacity and DPPH scavenging methods were performed. Results of phytochemical analysis showed that the total phenolics content were the highest in the hydroethanolic extract of Arbutus unedo with 160.76 mg GAE g-1DM, and the flavonoids content were the highest for the hydroethanolic extracts of Inula viscosa with 489.77 mg QE g-1 DM. Also, it can be noted that Arbutus unedo, Argania spinosa, and Myrtus communis exhibited the most potent antioxidant activity respectively with 0.026; 0.043; 0.036 mg ml-1.
Aazza S (2021). Application of multivariate optimization for phenolic compounds and antioxidants extraction from Moroccan Cannabis sativa waste. Journal of Chemistry 2021:1-11. https://doi.org/10.1155/2021/9738656
Afrokh M, Tahrouch S, EL Mehrach K, Fahmi F, Ait Bihi M, Weber-Ravn H, … Tabyaoui M (2023). Ethnobotanical, phytochemical and antioxidant study of fifty aromatic and medicinal plants. Chemical Data Collections 43:100984. https://doi.org/10.1016/j.cdc.2022.100984
Ait lhaj Z, Taghzouti K, Bouyahya A, Diria G, Bakhy K, Bchitou R (2022). Phenolic composition and antioxidant activity of leaves of strawberry tree (Arbutus unedo l.) populations from Morocco. Phytotherapy 20:192-204. https://doi.org/10.3166/phyto-2021-0296
Alara OR, Abdurahman NH, Ukaegbu CI (2021). Extraction of phenolic compounds: A review. Current Research in Food Science 4:200-214. https://doi.org/10.1016/j.crfs.2021.03.011
Aryal S, Baniya MK, Danekhu K, Kunwar P, Gurung R, Koirala N (2019). Total phenolic content, flavonoid content and antioxidant potential of wild vegetables from western Nepal. Plants 8(4):96. https://doi.org/10.3390/plants8040096
Aydar AY, Aydın T, Yılmaz T, Kothakota A, Claudia Terezia S, Florin Leontin C, Pandiselvam R (2022). Investigation on the influence of ultrasonic pretreatment on color, quality and antioxidant attributes of microwave dried Inula viscosa (L.). Ultrasonics Sonochemistry 90:106184. https://doi.org/10.1016/j.ultsonch.2022.106184
Barros L, Carvalho AM, Ferreira ICFR (2011). Exotic fruits as a source of important phytochemicals: Improving the traditional use of Rosa canina fruits in Portugal. Food Research International 44(7):2233-2236. https://doi.org/10.1016/j.foodres.2010.10.005
Bebek Markovinović A, Brčić Karačonji I, Jurica K, Lasić D, Skendrović Babojelić M, Duralija B, … Bursać Kovačević D (2022). Strawberry tree fruits and leaves (Arbutus unedo l.) as raw material for sustainable functional food processing: a review. Horticulturae 8(10):881. https://doi.org/10.3390/horticulturae8100881
Ben Ahmed Z, Yousfi M, Viaene J, Dejaegher B, Demeyer K, Mangelings D, vander Heyden Y (2017). Seasonal, gender and regional variations in total phenolic, flavonoid, and condensed tannins contents and in antioxidant properties from Pistacia atlantica ssp. leaves. Pharmaceutical Biology 55(1):1185-1194. https://doi.org/10.1080/13880209.2017.1291690
Benkirane C, Mansouri F, Ben Moumen A, Taaifi Y, Melhaoui R, Caid HS, … Abid M (2023). Phenolic profiles of non-industrial hemp (Cannabis sativa L.) seed varieties collected from four different Moroccan regions. International Journal of Food Science Technology 58(3):1367-1381. https://doi.org/10.1111/ijfs.16298
Brand-Williams W, Cuvelier ME, Berset C (1995). Use of a free radical method to evaluate antioxidant activity. Food Science and Technology 28(1):25-30. https://doi.org/10.1016/S0023-6438(95)80008-5
Çalişkan B, Cengiz Çalişkan A (2021). Antioxidant and oxidative stress. in antioxidants - benefits, sources, mechanisms of action. IntechOpen. https://doi.org/10.5772/intechopen.96643
Cheng Z, Li Y (2004). Reducing power: the measure of antioxidant activities of reductant compounds? Redox Report 9(4):213-217. https://doi.org/10.1179/135100004225005994
Chib A, Gupta N, Bhat A, Anjum N, Yadav G (2020). Role of antioxidants in food. International Journal of Chemical Studies 8(1):2354-2361. https://doi.org/10.22271/chemi.2020.v8.i1aj.8621
Forman HJ, Zhang H (2021). Targeting oxidative stress in disease: promise and limitations of antioxidant therapy. Nature Reviews Drug Discovery 20(9):689-709. https://doi.org/10.1038/s41573-021-00233-1
Habachi E, Rebey IB, Dakhlaoui S, Hammami M, Sawsen S, Msaada K, … Bourgou S (2022). Arbutus unedo: innovative source of antioxidant, anti-inflammatory and anti-tyrosinase phenolics for novel cosmeceuticals. Cosmetics 9(6):143. https://doi.org/10.3390/cosmetics9060143
Hazrati S, Hosseini SJ, Ebadi MT, Nicola S (2022). Evolution of phytochemical variation in myrtle (Myrtus communis l.) organs during different phenological stages. Horticulturae 8(9):757. https://doi.org/10.3390/horticulturae8090757
Heim KE, Tagliaferro AR, Bobilya DJ (2002). Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships. The Journal of Nutritional Biochemistry 13(10):572-584. https://doi.org/10.1016/S0955-2863(02)00208-5
Kapadia P, Newell AS, Cunningham J, Roberts MR, Hardy JG (2022) Extraction of high-value chemicals from plants for technical and medical applications. International Journal of Molecular Sciences 23(18):10334. https://doi.org/10.3390/ijms231810334
Lourenço SC, Moldão-Martins M, Alves VD (2019). antioxidants of natural plant origins: from sources to food industry applications. Molecules 24(22):4132. https://doi.org/10.3390/molecules24224132
Milkovic L, Cipak Gasparovic A, Cindric M, Mouthuy PA, Zarkovic N (2019). Short overview of ROS as cell function regulators and their implications in therapy concepts. Cells 8(8):793. https://doi.org/10.3390/cells8080793
Molyneux P (2004). The use of the stable free radical diphenylpicryl-hydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology 26(2):211-219.
Ngoune Liliane T, Shelton Charles M (2020). Factors affecting yield of crops. in agronomy - climate change and food security. IntechOpen. https://dx.doi.org/10.5772/intechopen.90672
Osman MA, Mahmoud GI, Shoman SS (2020). Correlation between total phenols content, antioxidant power and cytotoxicity. Biointerface Research in Applied Chemistry 11(3):10640-10653. https://doi.org/10.33263/briac113.1064010653
Özbek HN, Halahlih F, Göğüş F, Koçak Yanık D, Azaizeh H (2020). Pistachio (Pistacia vera L.) hull as a potential source of phenolic compounds: evaluation of ethanol–water binary solvent extraction on antioxidant activity and phenolic content of pistachio hull extracts. Waste and Biomass Valorization 11(5):2101-2110. https://doi.org/10.1007/s12649-018-0512-6
Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, … Bitto A (2017). Oxidative stress: harms and benefits for human health. Oxidative Medicine and Cellular Longevity 2017:8416763. https://doi.org/10.1155/2017/8416763
Prieto P, Pineda M, Aguilar M (1999). Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of Vitamin E1. Analytical Biochemistry 269(2):337-341. https://doi.org/10.1006/abio.1999.4019
Rankou H, Culham A, Jury SL, Christenhusz MJM (2013). The endemic flora of Morocco. Phytotaxa 78(1):1-27. https://doi.org/10.11646/phytotaxa.78.1.1
Reza M, Ardekani S, Khanavi M, Hadjiakhoondi A (2010). Comparison of antioxidant activity and total phenol contents of some date seed varieties from Iran. Iranian Journal of Pharmaceutical Research 9(2):141-146.
Rodríguez-Bernaldo de Quirós A, Lage-Yusty MA, López-Hernández J (2010). Determination of phenolic compounds in macroalgae for human consumption. Food Chemistry 121(2):634-638. https://doi.org/10.1016/j.foodchem.2009.12.078
Selaimia A, Azouz M, Chouikh A, Zga N, Besbes N (2020). Phytochemical study, antioxidant and antimicrobial activities of flavonoids and diethyl ether extracts from leaves and seeds of medicinal plant of Algeria flora: Retama monosperma (l.) boiss. PONTE International Scientific Researches Journal 76(4):42-52. https://doi.org/10.21506/j.ponte.2020.4.4
Sun A, Chi X, Yang X, Feng J, Li Y, Zhou J (2019). Applications and prospects of ultrasound-assisted extraction in Chinese herbal medicine. Open Access Journal of Biomedical Science 1(1):5-15. https://doi.org/10.38125/OAJBS.000103
Torres de Pinedo A, Peñalver P, Morales JC (2007). Synthesis and evaluation of new phenolic-based antioxidants: Structure–activity relationship. Food Chemistry 103(1):55-61. https://doi.org/10.1016/j.foodchem.2006.07.026
Yangui I, Younsi F, Ghali W, Boussaid M, Messaoud C (2021). Phytochemicals, antioxidant and anti-proliferative activities of Myrtus communis L. genotypes from Tunisia. South African Journal of Botany 137:35-45. https://doi.org/10.1016/j.sajb.2020.09.040
Yıldırım A, Şen A, Tuysuz M, Birteksöz Tan AS, Şenkardeş İ, Bitiş L (2022). In vitro investigation of antimicrobial, enzyme inhibitory and free radical scavenging activities of Inula salicina L. International Journal of Agriculture Environment and Food Sciences 6(3):389-395. https://doi.org/10.31015/jaefs.2022.3.7
Zeroual A, Sakar EH, Mahjoubi F, Chaouch M, Chaqroune A, Taleb M (2021). Effects of extraction technique and solvent on phytochemicals, antioxidant, and antimicrobial activities of cultivated and wild Rosemary (Rosmarinus officinalis L.) from Taounate region (northern Morocco). Biointerface Research in Applied Chemistry 12(6):8441-8452. https://doi.org/10.33263/BRIAC126.84418452
Zhang QW, Lin LG, Ye WC (2018). Techniques for extraction and isolation of natural products: A comprehensive review. Chinese Medicine 13(1):20. https://doi.org/10.1186/s13020-018-0177-x
Zhang Y, Cai P, Cheng G, Zhang Y (2022). A brief review of phenolic compounds identified from plants: their extraction, analysis, and biological activity. Natural Product Communications 17(1):1-14. https://doi.org/10.1177/1934578X211069721
How to Cite
Copyright (c) 2023 Anas EL MEKKAOUI, Mohamed KHAMAR, Chaimae SLIMANI, Abderrahman NOUNAH, Essediya CHERKAOUI, Fatima BENRADI, Chaimae RAIS
This work is licensed under a Creative Commons Attribution 4.0 International License.
Papers published in Notulae Scientia Biologicae are Open-Access, distributed under the terms and conditions of the Creative Commons Attribution License.
© Articles by the authors; licensee SMTCT, Cluj-Napoca, Romania. The journal allows the author(s) to hold the copyright/to retain publishing rights without restriction.
Open Access Journal - the journal offers free, immediate, and unrestricted access to peer-reviewed research and scholarly work, due SMTCT supports to increase the visibility, accessibility and reputation of the researchers, regardless of geography and their budgets. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author.