New insights regarding the taxonomy and phylogeography of Onobrychis montana DC. subsp. transsilvanica (Fabaceae)
DOI:
https://doi.org/10.55779/nsb15411730Keywords:
Carpathians, microsatellites, Onobrychis, phylogeographic groups, taxonomyAbstract
Onobrychis montana DC. Subsp. transsilvanica (Simonk.) Jáv. (Fabaceae) is a plant taxon, endemic to the South-Eastern Carpathians, whose taxonomic status is treated inconsistently by the classic taxonomy based on morphological traits. In a previous study, by sequencing several chloroplast DNA regions and AFLP genotyping, we tried to elucidate the controversy concerning its taxonomic status in relation to the more widespread O. montana DC., and determined its phylogeographic structure within the Carpathians. The present study, based on DNA fingerprinting by seven SSR markers, brings more insights on this problematic taxon by conferring higher resolution and sub-clustering previously identified phylogeographic groups. However, the newly SSR genetic data are in agreement with molecular evidence revealed by AFLP and chloroplastic SNPs and do not support O. montana DC. Subsp. transsilvanica (Simonk.) Jáv. As a distinct species from O. montana DC.
Metrics
References
Băcilă I, Şuteu D, Coldea G (2015). Genetic divergence and phylogeography of the alpine plant taxon Onobrychis transsilvanica (Fabaceae). Botany 93:257-266. https://doi.org/10.1139/cjb-2014-0175
Chytrý M, Dražil T, Hájek M, Kalníková V, Preislerová Z, Šibík J, … Vymazalová M (2015). The most species-rich plant communities in the Czech Republic and Slovakia (with new world records). Preslia 87:217-278. https://www.preslia.cz/P153Chytry_lowres.pdf
Cieślak E, Ilnicki T, Flis M (2000). Cytotaxonomical studies on the Caltha palustris complex (Ranunculaceae) in Poland. Preliminary report. Acta Biologica Cracoviensia Series Botanica 42(1):121-129.
Ciocârlan V (2009). Flora ilustrată a României - Pteridophyta et Spermatophyta. Ceres Press, Bucharest.
Coldea G, Stoica IA, Puşcaş M, Ursu T, Oprea A, Intrabiodiv Consortium (2009). Alpine–subalpine species richness of the Romanian Carpathians and the current conservation status of rare species. Biodiversity and Conservation 18:1441-1458. https://doi.org/10.1007/s10531-008-9488-z
Costa R, Pereira G, Garrido I, Tavares-de-Sousa MM, Espinosa F (2016). Comparison of RAPD, ISSR, and AFLP molecular markers to reveal and classify orchardgrass (Dactylis glomerata L.) germplasm variations. PLoS ONE 11(4):e0152972. https://doi.org/10.1371/journal.pone.0152972
Dessalegn Y, Herselman L, Labuschagne M (2009). Comparison of SSR and AFLP analysis for genetic diversity assessment of Ethiopian arabica coffee genotypes. South African Journal of Plant and Soil 26(2):119-125. https://doi.org/10.1080/02571862.2009.10639943
Dupanloup I, Schneider S, Excoffier L (2002). A simulated annealing approach to define the genetic structure of populations. Molecular Ecology 11(12):2571-2581. https://doi.org/10.1046/j.1365-294X.2002.01650.x
Ehrich D (2006). AFLPdat: a collection of R functions for convenient handling of AFLP data. Molecular Ecology Notes 6(3):603-604. https://doi.org/10.1111/j.1471-8286.2006.01380.x
Evanno G, Regnaut S, Goudet J (2005). Detecting the number of clusters of individuals using the software structure: a simulation study. Molecular Ecology 14(8):2611-2620. https://doi.org/10.1111/j.1365-294X.2005.02553.x
Garcia AA, Benchimol LL, Barbosa AM, Geraldi IO, Souza Jr CL, Souza APD (2004). Comparison of RAPD, RFLP, AFLP and SSR markers for diversity studies in tropical maize inbred lines. Genetics and Molecular Biology 27:579-588. https://doi.org/10.1590/S1415-47572004000400019
Gillaspie AG Jr, Hopkins MS, Dean RE (2005). Determining genetic diversity between lines of Vigna unguiculata subspecies by AFLP and SSR markers. Genetic Resources and Crop Evolution 52:245-247. https://doi.org/10.1007/s10722-004-6693-9
Hammer Ø, Harper DAT, Ryan PD (2001). Paleontological statistics software package for education and data analysis. Palaeontologia Electronica 4(1):1-9. http://palaeo-electronica.org/2001_1/past/issue1_01.htm
Huntley H, Birks HJB (1983). An atlas of past and present pollen maps for Europe 0-13.000 Years Ago. Cambridge University Press, Cambridge.
Hurdu BI, Escalante T, Pușcaș M, Novikoff A, Bartha L, Zimmermann NE (2016). Exploring the different facets of plant endemism in the South-Eastern Carpathians: a manifold approach for the determination of biotic elements, centres and areas of endemism. Botanical Journal of the Linnean Society 119(3):649-672. https://doi.org/10.1111/bij.12902
Hurdu BI, Pușcaș M, Turtureanu PD, Niketić M, Coldea G, Zimmermann NE (2012). Patterns of plant endemism in the Romanian Carpathians (South - Eastern Carpathians). Contribuții Botanice 47:25-38.
Huson DH, Bryant D (2006). Application of phylogenetic networks in evolutionary studies. Molecular Biology and Evolution 23(2):254-267. https://doi.org/10.1093/molbev/msj030
Jaccard P (1901). Distribution de la Flore Alpine dans le Bassin des Dranses et dans quelques régions voisines. Bulletin de la Société Vaudoise des Sciences Naturelles 37(140):241-272. https://doi.org/10.1111/j.1469-8137.1912.tb05611.x
Kolář F, Fuxová G, Záveská E, Nagano AJ, Hyklová L, Lučanová M, … Marhold K (2016). Northern glacial refugia and altitudinal niche divergence shape genome-wide differentiation in the emerging plant model Arabidopsis arenosa. Molecular Ecology 25(16):3929-3949. https://doi.org/10.1111/mec.13721
Maguire TL, Peakall R, Saenger P (2002). Comparative analysis of genetic diversity in the mangrove species Avicennia marina (Forsk.) Vierh. (Avicenniaceae) detected by AFLPs and SSRs. Theoretical and Applied Genetics 104(2-3):388-398. https://doi.org/10.1007/s001220100724
Marulanda ML, López AM, Aguilar SB (2007). Genetic diversity of wild and cultivated Rubus species in Colombia using AFLP and SSR markers. Crop Breeding and Applied Biotechnology 7(3):242-252.
Mason AS (2015). SSR Genotyping. In: Batley J (Ed). Plant Genotyping. Springer, New York, pp 77-89.
Mitka J, Boroń P, Novikoff A, Wróblewska A, Binkiewicz B (2016). Two major groups of chloroplast DNA haplotypes in diploid and tetraploid Aconitum subgen. Aconitum in the Carpathians. Modern Phytomorphology 9:5-15. https://doi.org/10.5281/zenod o.159700
Mráz P, Barabas D, Lengyelová L, Turis P, Schmotzer A, Janišová M, Ronikier M (2016). Vascular plant endemism in the Western Carpathians: spatial patterns, environmental correlates and taxon traits. Botanical Journal of the Linnean Society 119(3):630-648. https://doi.org/10.1111/bij.12792
Mráz P, Ronikier M (2016). Biogeography of the Carpathians: evolutionary and spatial facets of biodiversity. Biological Journal of the Linnean Society 119(3):528-559. https://doi.org/10.1111/bij.12918
Nei M (1987). Molecular evolutionary genetics. Columbia University Press, New York.
Ozenda P (1985). La végétation de la chaîne alpine dans l'espace montagnard européen. Masson, Paris.
Pawłowski B (1970). Remarques sur l’endémisme dans la flore des Alpes et des Carpates. Vegetatio 21:181-243. https://doi.org/10.1007/BF02269663
Pritchard JK, Stephens M, Donnelly P (2000). Inference of population structure using multilocus genotype data. Genetics 155(2):945-959. https://doi.org/10.1534/genetics.116.195164
Rawat A, Barthwal S, Ginwal HS (2014). Comparative assessment of SSR, ISSR and AFLP markers for characterization of selected genotypes of Himalayan Chir pine (Pinus roxburghii Sarg.) based on resin yield. Silvae Genetica 63(3):94-109. https://doi.org/10.1515/sg-2014-0013
Ronikier M (2011). Biogeography of high-mountain plants in the Carpathians: an emerging phylogeographical perspective. Taxon 60(2):373-389. https://doi.org/10.1002/tax.602008
Ronikier M, Cieślak E, Korbecka G (2008). High genetic differentiation in the alpine plant Campanula alpina Jacq. (Campanulaceae): Evidence for glacial survival in several Carpathian regions and long-term isolation between the Carpathians and the Alps. Molecular Ecology 17(7):1763-1775. https://doi.org/10.1111/j.1365-294X.2008.03664.x
Ronikier M, Zalewska-Gałosz J (2014). Independent evolutionary history between the Balkan ranges and more northerly mountains in Campanula alpina sl (Campanulaceae): genetic divergence and morphological segregation of taxa. Taxon 63(1):116-131. https://doi.org/10.12705/631.4
Sârbu I, Ştefan N, Oprea A (2013). Plante vasculare din România, Determinator ilustrat de teren. Victor B. Victor Press, Bucharest.
Schönswetter P, Tribsch A (2005). Vicariance and dispersal in the alpine perennial Bupleurum stellatum L. (Apiaceae). Taxon 54(3):725-732. https://doi.org/10.2307/25065429
Sorkheh K, Dehkordi MK, Ercisli S, Hegedus A, Halász J (2017). Comparison of traditional and new generation DNA markers declares high genetic diversity and differentiated population structure of wild almond species. Scientific Reports 7(1):5966. https://doi.org/10.1038/s41598-017-06084-4
Španiel S, Marhold K, Filová B, Zozomová-Lihová J (2011). Genetic and morphological variation in the diploid-polyploid Alyssum montanum in Central Europe: taxonomic and evolutionary considerations. Plant Systematics and Evolution 294:1-25. https://doi.org/10.1007/s00606-011-0438-y
Stodart BJ, Mackay M, Raman H (2005). AFLP and SSR analysis of genetic diversity among landraces of bread wheat (Triticum aestivum L. em.Thell) from different geographic regions. Australian Journal of Agricultural Research 56(7):691-697. https://doi.org/10.1071/AR05015
Șuteu D, Băcilă I, Stoica A-I, Balázs ZR, Pușcaș M, Coldea G (2023). Phylogeographic pattern of the high-alpine plant species Eritrichium nanum (Boraginaceae) within the Carpathians. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 51(1):12971. https://doi.org/10.15835/nbha51112971
Sutkowska A, Boroń P, Warzecha T, Dębowski J, Mitka J (2017). Hybridization and introgression among three Aconitum (Ranunculaceae) species of different ploidy levels in the Tatra Mountains (Western Carpathians). Plant Species Biology 32(4):292-303. https://doi.org/10.1111/1442-1984.12162
Szela̧g Z (2006) Taxonomic revision of Hieracium sect. Cernua (Asteraceae) in the Carpathians, Sudetes and Alps. Polish Botanical Journal 51(2):97-153.
Tasenkevich L (1998). Flora of the Carpathians: checklist of the native vascular plant species. National Academy of Sciences of Ukraine, State Museum of Natural History, Lviv.
Vieira MLC, Santini L, Diniz AL, Munhoz CdeF (2016). Microsatellite markers: what they mean and why they are so useful. Genetics and Molecular Biology 39(3):312-328. https://doi.org/10.1590/1678-4685-GMB-2016-0027
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Ioan BĂCILĂ, Dana ŞUTEU, Ana COSTE, Zoltan R. BALÁZS, Gheorghe COLDEA
This work is licensed under a Creative Commons Attribution 4.0 International License.
Papers published in Notulae Scientia Biologicae are Open-Access, distributed under the terms and conditions of the Creative Commons Attribution License.
© Articles by the authors; licensee SMTCT, Cluj-Napoca, Romania. The journal allows the author(s) to hold the copyright/to retain publishing rights without restriction.
License:
Open Access Journal - the journal offers free, immediate, and unrestricted access to peer-reviewed research and scholarly work, due SMTCT supports to increase the visibility, accessibility and reputation of the researchers, regardless of geography and their budgets. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author.