Evaluation of mosquito larvicidal activity of green synthesized crystalline silver nanoparticles using leave and fruit extracts of Phyllanthus acidus L. (Phyllanthaceae)


  • Arpita GOPE The University of Gour Banga, Department of Zoology, Laboratory of Parasitology, Vector Biology, Nanotechnology, Malda, West Bengal (IN)
  • Anjali RAWANI The University of Gour Banga, Department of Zoology, Laboratory of Parasitology, Vector Biology, Nanotechnology, Malda, West Bengal (IN)
  • Priyajit CHATTERJEE The University of Burdwan, University Science Instrument Centre, Burdwan, West Bengal (IN)




Culex quinquefasciatus, Culex vishnui, larvicidal activity, Phyllanthus acidus, probit analysis, silver nanoparticles


The present study evaluates the potentiality of green silver nanoparticles from the leaves and fruit extracts of Phyllanthus acidus L. against third instar larvae of two vector mosquitoes namely Culex quinquefasciatus and Culex vishnui. Various spectroscopic techniques were used to characterize the synthesized silver nanoparticles (AgNPs). Synthesized silver nanoparticles from both the leaf and fruits of P. acidus were spherical to quasi-spherical in shape and showed Surface Plasmon Resonance (SPR) bands at 420 and 409 nm respectively. In larvicidal bioassay with synthesized AgNPs from the leave of P. acidus, 100% mortality was observed at 20 ppm against third instar larvae of both the mosquito species with LC50 values of 1.64 and 0.87 ppm respectively. 100% mortality was observed in 5 ppm concentration against both Cx. quinquefasciatus and Cx. vishnui in synthesized AgNPs from fruits of P. acidus with LC50 values of 1.62 and 1.24 ppm respectively. The above findings suggest that the AgNPs synthesized from P. acidus leaves and fruit extracts have the potential to be employed in vector mosquito population control.


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Abbott WS (1925). A method of computing the effectiveness of an insecticide. Journal of Economic Entomology 18:265-267.

Barraud PJ (1934). The fauna of British India, including Ceylon and Burma. Diptera vol. V. Family Culicidae. Tribes Megarhinini and Culicini. London: Taylor and Francis; pp 463.

Bhat R, Ganachari S, Deshpande R, Ravindra G, Venkataraman A (2013). Rapid biosynthesis of silver nanoparticles using Areca nut (Areca catechu) extract under microwave-assistance. Journal of Cluster Science 24(1):107-114. https://doi.org/10.1007/s10876-012-0519-2

Center for New Crops & Plants Products (2011). Otaheite Gooseberry, Purdue University.

Chandra G (2000). Mosquito. Calcutta: Sribhumi Publishing Co pp 1-102.

Christophers SR (1944). The fauna of British India, including Ceylon and Burma. Diptera, vol. iv Family Culicidae. Tribes Anophelini. London: Taylor and Francis; pp 371. 22.

Das R, Ali MdE, Hamid SBA (2014). Current applications of X – Ray powder diffraction – A review. Reviews on Advanced Materials Science 38(2):95-109.

Edible (2008). An Illustrated Guide to The World´s Food Plants. National Geographic Books, pp 110.

Finney DJ (1971). Probit Analysis. 3rd Edition. Cambridge University Press.

Ghosh A, Rawani A, Mondal RP, Chandra G (2022) Mosquito larvicidal and antimicrobial activities of synthesized silver nanoparticles (AgNP) using mature fruit extract of Cestrum diurnum L. Indian Journal of Natural Products and Resources 12(4):592-599.

Goodsell DS (2004). The quest for nanotechnology. Bio-nanotechnology: Lessons from Nature 5:1-8. https://doi.org/10.1002/0471469572.ch1

Haldar KM, Haldar B, Chandra G (2013). Fabrication, characterization and mosquito larvicidal bioassay of silver nanoparticles synthesized from aqueous fruit extract of putranjva, Drypetes roxburghii (Wall.). Parasitology Research 112(4):1451-1459.

Ibrahim IK, Hussain SM, Obaidi YMA (2015). Extraction of cellulose nano crystalline from cotton by ultrasonic and its morphological and structural characterization. International Materials Chemistry and Physics 1(2):99-109.

Jain NK, Lodhi S, Jain A, Nahata A, Singhai AK (2011) Effects of Phyllanthus acidus (L.) skeels fruit on carbon tetrachloride-induced acute oxidative damage in livers of rats and mice. Journal of Chinese Integrative Medicine 9(1):49-56. https://doi.org/10.3736/jcim20110109

Kamaraj C, Bagavan A, Rahuman AA, Zahir AA, Elango G, Pandiyan G (2009). Larvicidal potential of medicinal plant extracts against Anopheles subpictus Grassi and Culex tritaeniorhynchus Giles (Diptera: Culicidae). Parasitology Research 104(5):1163-1171. https://doi.org/10.1007/s00436-008-1306-8

Kvitek L, Panacek A, Soukupova J, Kolar M, Vecerova R (2008). Effect of surfectant and polymer on stability and antibacterial activity of silver nanoparticles. Journal of Physical Chemistry 112:5825-5834. https://doi.org/10.1021/jp711616v

Leeya Y, Mulvany MJ, Queiroz EF, Marston A, Hostettmann K, Jansakul C (2010). Hypotensive activity of an n – butanol extract and their purified compounds from leaves of Phyllanthus acidus (L.) Skeels in rats. European Journal of Pharmacology 649(1-3):301-313. https://doi.org/10.1016/j.ejphar.2010.09.038

Madangopal N, Mahalingam L, Palanisamy S (2017). Effect of phyto synthesized silver nanoparticles on developmental stages of malaria vector, Anopheles stephensi and dengue vector, Aedes aegypti. Egyptian Journal of Basic and Applied Sciences 4(3):212-218. https://doi.org/10.1016/j.ejbas.2017.04.005

Maquart PO, Chann L, Boyer S (2022). Culex vishnui (Diptera: Culicidae): An overlooked vector of arboviruses in South-East Asia. Journal of Medical Entomology 59(4):1144-1153. https://doi.org/10.1093/jme/tjac044

Matthew AA, Cameron W, Raston CL (2006). Green chemistry and the health implications of nanoparticles. Green Chemistry 8:417-432.

Miller W, Bailey LH (2011). Cyclopedia of American Horticulture: Comprising Suggestions for Cultivation of Horticulture. The Macmillan Company, pp 1318.

Mohanpuria P, Rana NK, Yadav SK (2008). Biosynthesis of nanoparticles: technological concepts and future applications. Journal of Nanoparticle Research 10:507-517. https://doi.org/10.1007/s11051-007-9275-x

Morejón B, PilaquingaF, Domenech F, Ganchala D, Debut A, Neira M (2018). Larvicidal activity of silver nanoparticles synthesized using extracts of Ambrosia arborescens (Asteraceae) to control Aedes aegypti L. (Diptera: Culicidae). Journal of Nanotechnology Article 6917938. https://doi.org/10.1155/2018/6917938

Mulvancy P (1996). Surface plasmon spectroscopy of nano sized metal particles. Langmuir 12(3):788-800.

Pal H, Sharma V (2015). Thermal conductivity of carbon nanotube – silver composite. Transactions of Nonferrous Metals Society of China 25:154-161. https://doi.org/10.1016/S1003-6326(15)63590-7

Pandya M, Ansu AK, Sharma RK (2022). Copper based nano materials-enhanced phase change materials with great potential for improved thermal energy storage properties. Materials Today: Proceedings 63:786-789. https://doi.org/10.1016/j.matpr.2022.05.503

Parthiban E, Manivannan N, Ramanibai R, Mathivanan N (2019). Green synthesis of silver-nanoparticles from Annona reticulata leaves aqueous extract and its mosquito larvicidal and anti-microbial activity on human pathogens. Biotechnology Reports 21. https://doi.org/10.1016/j.btre.2018.e00297.

Pilaquinga F, Morejón B, Ganchala D, Morey J, Piña NN, Debut A, Neira M (2019). Green synthesis of silver nanoparticles using Solanum mammosum L. (Solanaceae) fruit extract and their larvicidal activity against Aedes aegypti L. (Diptera: Culicidae). PLOS One 14(10):e0224109. https://doi.org/10.1371/journal.pone.0224109

Potera C (2008). Pesticides: in search of a better mosquito repellent. Environmental Health Perspectives 116(8):A337. https://doi.org/10.1289/ehp.116-a337

Rai H, Prem RM, Singh AP, Tejavath KK (2020). Biosynthesis of silver nanoparticles using Cucumis prophetarum aqueous leaf extract and their antibacterial and antiproliferative activity against cancer cell lines. ACS Omega. https://dx.doi.org/10.1021/acsomega.0c00155

Rawani A, Ghosh A, Chandra G (2013). Mosquito larvicidal and antimicrobial activity of synthesized nano-crystalline silver particles using leaves and green berry extract of Solanum nigrum L. (Solanaceae: Solanales). Acta Tropica 128(3):613-22. http://dx.doi.org/10.1016/j.actatropica.2013.09.007.

Rawani A (2017). Mosquito larvicidal activity of green silver nanoparticle synthesized from extract of bud of Polianthus tuberosa L. The International Journal of Nanotechnology and Applications 11(1):17 -28.

Rodriguez YFB, Reyes CAR, Campos JST, Hernandez JG, Gamir JR (2021). Infrared spectroscopy coupled with chemometrics in coffee post – harvest processes as complement to the sensory analysis. Lebensmittel-Wissenschaft & Technologie 145:111304. https://doi.org/10.1016/j.lwt.2021.111304

Sinha I, Singh M, Mandal RK (2009). Role of pH in the green synthesis of silver nanoparticles. Materials Letters 63(3-4):425-427. http://doi.org/10.1016/j.matlet.2008.10.067.

Soni N, Prakash S (2014). Microbial synthesis of nanosilver and nanogold for mosquito control. Annals of Microbiology 64:1099-1111. https://doi.org/10.1007/s13213-013-0749-z.

Sousa M, Ousingsawat J, Seitz R, Puntheeranurak S, Regalado A, Schmidt A, Grego T, Jansakul C (2006). An extract from the medicinal plant Phyllanthus acidus and its isolated compounds induce airway chloride secretion: a potential treatment for cystic fibrosis. Molecular Pharmacology 71(1):366-376. https://doi.org/10.1124/mol.106.025262

Stuart B (2004). Infrared Spectroscopy: Fundamentals and Applications. John Wiley & Sons, Ltd.

Sundaravadivelan C, Nalini M (2012). Biolarvicidal effect of phyto-synthesized silver nanoparticles using Pedilanthus tithymaloides (L.) Poit stem extract against the dengue vector Aedes aegypti L. (Diptera; ulicidae). Asian Pacific Journal of Tropical Biomedicine 17:1-8.

Veerakumar K, Govindarajan M (2014). Adulticidal properties of synthesized silver nanoparticles using leaf extracts of Feronia elephantum (Rutaceae) against filariasis, malaria, and dengue vector mosquitoes. Parasitology Research 113(11):4085-4096. https://doi.org/10.1007/s00436-014-4077-4

Vivekanandhan P, Senthil-Nathan, Sengottayan, Shivakumar MS (2017). Larvicidal, pupicidal and adult smoke toxic effects of Acanthospermum hispidum (DC) leaf crude extracts against mosquito vectors. Physiological and Molecular Plant Pathology 101:156-162. http://dx.doi.org/10.1016/j.pmpp.2017.05.005

World Health Organization (1997). Validation of elimination of lymphatic filariasis as a public health problem.

World Health Organization (2002). Lymphatic filariasis – the disease and its control. Technical report no. 71. World Health Organization, Geneva.

World Health Organization (2005). Communicable Disease Tool Kit. World Health Organization, WHO/CDS/2005.26, Sudan.

World Health Organization (2022) Lymphatic filariasis fact sheet. www.who.int/news-room/fact-sheets/detail/lymphatic filariasis



How to Cite

GOPE, A., RAWANI, A., & CHATTERJEE, P. (2023). Evaluation of mosquito larvicidal activity of green synthesized crystalline silver nanoparticles using leave and fruit extracts of Phyllanthus acidus L. (Phyllanthaceae) . Notulae Scientia Biologicae, 15(4), 11722. https://doi.org/10.55779/nsb15411722



Research articles
DOI: 10.55779/nsb15411722