Molecular and morphological characterization of Globodera rostochiensis (Wollenweber, 1923) Skarbilovich, 1959 from Egypt

  • Sanaa A. HAROON Fayoum University, Faculty of Agriculture, Plant Protection Department, Fayoum (EG)
  • Zafar HANDOO Mycology and Nematology Genetic Diversity and Biology Laboratory, USDA, ARS, Northeast Area, Beltsville, MD 20705 (US)
  • Mihail KANTOR Mycology and Nematology Genetic Diversity and Biology Laboratory, USDA, ARS, Northeast Area, Beltsville, MD 20705 (US)
  • Andrea SKANTAR Mycology and Nematology Genetic Diversity and Biology Laboratory, USDA, ARS, Northeast Area, Beltsville, MD 20705 (US)
  • Maria HULT Mycology and Nematology Genetic Diversity and Biology Laboratory, USDA, ARS, Northeast Area, Beltsville, MD 20705 (US)
Keywords: Egypt, Globodera rostochiensis, PCN, potato

Abstract

The golden potato cyst nematode, Globodera rostochiensis (Wollenweber, 1923) Skarbilovich (1959) is a damaging soilborne quarantine pest of Solanum tuberosum (potato) and other solanaceous crops worldwide. In spring of 2021 a survey was conducted in area of Abo El Matamer, Bahera governorates in Egypt. Soil samples were taken in zigzag pattern throughout 65 acres of potato cultivated land and processed in Nematology lab, Fayoum University, Egypt. In June 2021, two hundred soil samples were collected from nearby areas to evaluate the distribution of this potato cyst nematode in other cultivated land located in area of first infection but fortunately the golden potato cyst nematode was not detected from neighboring locations. The nematode species was identified by both morphological and molecular means as Globodera rostochiensis. To our knowledge this is the first molecular and morphological characterization of G. rostochiensis from Egypt.

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References

Ayoub SM (1980). Plant nematology: An agricultural training aid (No. 632.6513 A983). NemaAid Publications.

Gartner U, Hein I, Brown LH, Chen X, Mantelin S, Sharma SK, … Blok VC (2021). Resisting potato cyst nematodes with resistance. Frontiers in Plant Science 12:483. https://doi.org/10.3389/fpls.2021.661194

Golden AM (1986). Morphology and identification of cyst nematodes. In: Lamberti F, Taylor CE (Eds). Cyst Nematodes. New York: Plenum Press, pp 23-45.

Golden AM (1990). Preparation and mounting nematodes for microscopic observations. In: Zuckerman BM, Mai WF, Krusberg LR (Eds). Plant nematology Laboratory Manual. Amherst, MA: University of Massachusetts Agricultural Experiment Station pp 197-205.

Golden AM, Ellington DMS (1972). Re-description of Heterodera rostochiensis (Nematoda: Heteroderidae), with a key and notes on closely related species. Proceedings of the Helminthological Society of Washington 39:64-78.

Hooper DJ (1970). Handling, fixing, staining, and mounting nematodes. In: Southey JF (Ed). Laboratory Methods for Work with Plant and Soil Nematodes. 5th Edition. London: Her Majesty’s Stationery Office, pp 39-54.

Ibrahim IKA, Handoo ZA, Basyony ABA (2017). The cyst nematodes Heterodera and Globodera species in Egypt. Pakistan Journal of Nematology 35(2):151-154.

Mulvey RH, Golden AM (1983). An illustrated key to the cyst-forming genera and species of Heteroderidae in the Western Hemisphere with species morphometrics and distribution. Journal of Nematology 15(1):1-59.

Skantar AM, Handoo ZA, Kantor MR, Carta LK, Faghihi J, Ferris V (2020). Characterization of Vittatidera zeaphila (Nematoda: Heteroderidae) from Indiana with molecular phylogenetic analysis of the genus. Journal of Nematology 52:1-8. https://doi.org/10.21307/jofnem-2020-024

Subbotin SA, Mundo-Ocampo M, Baldwin JG (2010). Systematics of the genus Globodera. Chapter 6. In: Hunt DJ, Perry RN (Eds). Systematics of Cyst Nematodes (Nematoda: Heteroderinae). Nematology Monographs and Perspectives 8A. Leiden, The Netherlands, Brill pp 107-177.

Subbotin SA, Akanwari J, Nguyen CN, Cid del Prado Vera I, Chihtambar JJ, Inserra RN, Chizhov VN (2017). Molecular characterization and phylogenetic relationships of cystoid nematodes of the family Heteroderidae (Nematoda: Tylenchida). Nematology 19:1065-1081 https://doi.org/10.1163/15685411-00003107

Turner SJ, Subbotin SA (2013). Cyst nematodes. In: Perry RN, Moens M (Eds). Plant Nematology. Wallingford: CAB International, pp 109-143.

Published
2021-11-11
How to Cite
HAROON, S. A., HANDOO, Z., KANTOR, M., SKANTAR, A., & HULT, M. (2021). Molecular and morphological characterization of Globodera rostochiensis (Wollenweber, 1923) Skarbilovich, 1959 from Egypt. Notulae Scientia Biologicae, 13(4), 11083. https://doi.org/10.15835/nsb13411083
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DOI: 10.15835/nsb13411083