RAPD and ISSR Methods Used for Fingerprinting of Selected Accessions of Viburnum

  • Marcelina KRUPA-MAŁKIEWICZ West Pomeranian University of Technology in Szczecin, Department of Plant Genetics, Breeding and Biotechnology, 17 Słowackiego, 71-434 Szczecin, Poland
  • Miłosz Smolik West Pomeranian University of Technology in Szczecin, Department of Plant Genetics, Breeding and Biotechnology, 17 Słowackiego, 71-434 Szczecin, Poland
  • Anna BARNIAK West Pomeranian University of Technology in Szczecin, Department of Plant Genetics, Breeding and Biotechnology, 17 Słowackiego, 71-434 Szczecin, Poland
  • Beata SMOLIK West Pomeranian University of Technology in Szczecin, Department of Plant Physiology and Biochemistry, 17 Słowackiego, 71-434 Szczecin, Poland

Abstract

Randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers were used to investigate genetic variability within thirteen Viburnum species (Viburnum × hillieri; V. dilatatum; Viburnum × carlcephalum; V. opulus; V. hupehense; Viburnum× bodnantense; Viburnum × burkwoodii; V. sieboldii; Viburnum × globosum ‘Jermyns Globe’; V. alnifolium (lantanoides); V. plicatum ‘Sterile’; V. plicatum f. tomentosum and V. plicatum ‘Watanabe’) of wide geographical distribution, collected in the Dendrological Garden in Przelewice (the north-west part of Poland). Twenty-three RAPD and fourteen ISSR primers generated a total of 690 and 418 reproducible bands, respectively, and 39% (RAPD) and 55.5% (ISSR) of them were polymorphic for the two marker systems, which suggest high genetic variability within Viburnum genus. However, high numbers of genotype-specific bands, i.e. 60.9% (RAPD) and 44.5% (ISSR), were seen in Viburnum. Genetic similarity assessed within Viburnum species with the RAPD and ISSR analyses ranged from 6 to 42% and from 6 to 31%, respectively. Both RAPD and ISSR-based dendrograms clustered in five main groups. The Mantel test between two Nei’s similarity matrices gave correlation coefficient r=0.305*, showing low correlation between RAPD- and ISSR- based matrices. Thus, both marker systems were equally important for the genetic diversity analysis in Viburnum genus.

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Author Biographies

Marcelina KRUPA-MAŁKIEWICZ, West Pomeranian University of Technology in Szczecin, Department of Plant Genetics, Breeding and Biotechnology, 17 Słowackiego, 71-434 Szczecin, Poland
Department of Plant Genetic, Breeding and Biotechnology, Słowackiego 17 str. 71-434 Szczecin
Miłosz Smolik, West Pomeranian University of Technology in Szczecin, Department of Plant Genetics, Breeding and Biotechnology, 17 Słowackiego, 71-434 Szczecin, Poland
Department of Plant Genetic, Breeding and Biotechnology, Słowackiego 17 str. 71-434 Szczecin
Published
2014-09-22
How to Cite
KRUPA-MAŁKIEWICZ, M., Smolik, M., BARNIAK, A., & SMOLIK, B. (2014). RAPD and ISSR Methods Used for Fingerprinting of Selected Accessions of Viburnum. Notulae Scientia Biologicae, 6(3), 292-299. https://doi.org/10.15835/nsb639422
Section
Research article