Genetic Diversity in Commercial Rapeseed (Brassica napus L.) Varieties from Turkey as Revealed by RAPD

  • Özlem ÖZBEK Hitit University, Faculty of Arts and Sciences, Department of Biology, Ulukavak Mah. Ciftlik Cayiri Cd. No: 61, Çorum
  • Betül Uçar GIDIK Hitit University, Faculty of Arts and Sciences, Department of Biology, Ulukavak Mah. Ciftlik Cayiri Cd. No: 61, Çorum

Abstract

In cultivated commercial crop species, genetic diversity tends to decrease because of the extensive breeding processes. Therefore, germplasm of commercial crop species, such as Brassica napus L. should be evaluated and the genotypes, which have higher genetic diversity index, should be addressed as potential parental cross materials in breeding programs. In this study, the genetic diversity was analysed by using randomly amplified polymorphic DNA analysis (RAPD) technique in nine Turkish commercial rapeseed varieties. The RAPD primers (10-mer oligonucleotides) produced 51 scorable loci, 31 loci of which were polymorphic (60.78%) and 20 loci (39.22%) were monomorphic The RAPD bands were scored as binary matrix data and were analysed using POPGENE version 1.32. At locus level, the values of genetic diversity within population (Hs) and total (HT) were 0.15 and 0.19 respectively. The genetic differentiation (GST) and the gene flow (Nm) values between the populations were 0.20 and 2.05 respectively. The mean number of alleles (na), the mean number of effective alleles (nae), and the mean value of genetic diversity (He) were 2.00, 1.26, and 0.19 respectively. According to Pearson’s correlation, multiple regression and principal component analyses, eco-geographical conditions in combination had significant effect on genetic indices of commercial B. napus L. varieties were discussed.

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Author Biographies

Özlem ÖZBEK, Hitit University, Faculty of Arts and Sciences, Department of Biology, Ulukavak Mah. Ciftlik Cayiri Cd. No: 61, Çorum
Betül Uçar GIDIK, Hitit University, Faculty of Arts and Sciences, Department of Biology, Ulukavak Mah. Ciftlik Cayiri Cd. No: 61, Çorum
Published
2013-02-23
How to Cite
ÖZBEK, Özlem, & GIDIK, B. U. (2013). Genetic Diversity in Commercial Rapeseed (Brassica napus L.) Varieties from Turkey as Revealed by RAPD. Notulae Scientia Biologicae, 5(1), 114-119. https://doi.org/10.15835/nsb518911
Section
Biotechnology; Research Articles