The Investigation of Decontamination Effects of Ozone Gas on Microbial Load and Essential Oil of Several Medicinal Plants

  • Razieh VALI ASILL Ferdowsi University of Mashhad, Faculty of agriculture, Horticulture Department, Azadi Street, Mashhad
  • Majid AZIZI Ferdowsi University of Mashhad, Faculty of agriculture, Horticulture Department, Azadi Street, Mashhad
  • Maasome BAHREINI
  • Hossein AROUIEE Ferdowsi University of Mashhad, Faculty of agriculture, Horticulture Department, Azadi Street, Mashhad

Abstract

Today, Ozone as a disinfectant method, without putting on the harmful effects on human and plant products, it is alternative common methods for disinfection of plant material. The research as a factorial experiment was conducted on the basis of randomized complete block design with three replications and the effects of Ozone gas on decreasing the microbial load of some important medicinal plants include: Peppermint (Mentha piperita), Summer savory (Satureja hortensis), Indian valerian(Valeriana wallichii), Meliss (Melissa officinalis) and Iranian thyme (Zataria multiflora) were investigated. Medicinal plants leaves were treated with Ozone gas concentration 0.3, 0.6 and 0.9 ml/L at times of 10 and 30 then total count, coliform and mold and yeast of the samples were studied. The result showed that Ozone gas decreases microbial load of medicinal plants samples. But Ozone gas and Ozone gas in medicinal plants interaction effect had no effect on essential oil content. The lowest and the highest of microbial load were detected in samples treated with concentration of 0.9 ml/L of Ozone gas and control respectively. The highest and the lowest of microbial load were observed in Iranian thyme and Indian valerian respectively. Also result showed that Ozone gas treatment for 30 min had the greatest of effect in reducing the microbial load and 0.9 ml/L Ozone gas concentration had the lowest of microbial load. Results of this survey reflect that the use of Ozone as a method of disinfection for medicinal plants is a decontamination.

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Author Biographies

Razieh VALI ASILL, Ferdowsi University of Mashhad, Faculty of agriculture, Horticulture Department, Azadi Street, Mashhad
Majid AZIZI, Ferdowsi University of Mashhad, Faculty of agriculture, Horticulture Department, Azadi Street, Mashhad
Maasome BAHREINI
Hossein AROUIEE, Ferdowsi University of Mashhad, Faculty of agriculture, Horticulture Department, Azadi Street, Mashhad
Published
2013-02-23
How to Cite
VALI ASILL, R., AZIZI, M., BAHREINI, M., & AROUIEE, H. (2013). The Investigation of Decontamination Effects of Ozone Gas on Microbial Load and Essential Oil of Several Medicinal Plants. Notulae Scientia Biologicae, 5(1), 34-38. https://doi.org/10.15835/nsb518297
Section
Plant Sciences; Ecology; Research Articles