The Influence of Explant Types and Orientation on in Vitro Culture

Kamnoon KANCHANAPOOM; Nararatn PROMSORN

doi:10.15835/nsb336120

The Influence of Explant Types and Orientation on in Vitro Culture

Authors

Kamnoon KANCHANAPOOM Prince of Songkla University, Faculty of Science, Department of Biology, Hat Yai, Songkhla 90112 (TH)
Nararatn PROMSORN Prince of Songkla University, Faculty of Agro-Industry, Department of Industrial Biotechnology, Hat Yai, Songkhla 90112 (TH)

DOI:

https://doi.org/10.15835/nsb336120

Abstract

Inflorescence, apical and lateral buds of Musa balbisiana ‘Kluai Hin’ (BBB group) were used to culture on MS medium supplemented with 22 μM BA and 15% (v/v) coconut water. Comparison of bud orientation showed that the best response of in vitro shoot tip proliferation was obtained with abaxial surface of buds lying down i.e. one side touching the medium (tilt). Mass propagation of shoot tips was obtained when cultured buds on MS medium containing 44 μM BA. Rooting was achieved by transferring the isolated shoots to MS basal medium without growth regulators. Rooted plantlets were acclimatized and successfully established in soil.

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Author Biographies

Kamnoon KANCHANAPOOM, Prince of Songkla University, Faculty of Science, Department of Biology, Hat Yai, Songkhla 90112

Assoc. Prof. Dr. Department of Biology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112

Nararatn PROMSORN, Prince of Songkla University, Faculty of Agro-Industry, Department of Industrial Biotechnology, Hat Yai, Songkhla 90112

Prince of Songkla University, Faculty of Agro-Industry, Department of Industrial Biotechnology, Hat Yai, Songkhla 90112, Thailand.

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Published

2011-08-25

How to Cite

KANCHANAPOOM, K., & PROMSORN, N. (2011). The Influence of Explant Types and Orientation on in Vitro Culture. Notulae Scientia Biologicae, 3(3), 89–92. https://doi.org/10.15835/nsb336120

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Issue

Vol. 3 No. 3 (2011)

Section

Research articles

CITATION

DOI: 10.15835/nsb336120

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Papers published in Notulae Scientia Biologicae are Open-Access, distributed under the terms and conditions of the Creative Commons Attribution License.

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