Therapeutic potential of caffeic acid and coumarin in modifying aluminum-induced hepatic injury in Wistar rats


  • Zehor CHOUARI University of Oran 1 Ahmed Ben Bella, Faculty of Natural and Life Sciences, Department of Biology, Laboratory of Experimental Biotoxicology, Biodepollution and Phytoremediation, Oran 31100 (DZ)
  • Omar KHAROUBI University of Oran 1 Ahmed Ben Bella, Faculty of Natural and Life Sciences, Department of Biology, Laboratory of Experimental Biotoxicology, Biodepollution and Phytoremediation, Oran 31100 (DZ)
  • Khadidja KESSAS University of Oran 1 Ahmed Ben Bella, Faculty of Natural and Life Sciences, Department of Biology, Laboratory of Experimental Biotoxicology, Biodepollution and Phytoremediation, Oran 31100 (DZ)
  • Wafaa LOUNIS University of Oran 1 Ahmed Ben Bella, Faculty of Natural and Life Sciences, Department of Biology, Laboratory of Experimental Biotoxicology, Biodepollution and Phytoremediation, Oran 31100 (DZ)
  • Anne VEJUX University of Bourgogne Franche-Comté, Team ‘Biochemistry of the Peroxisome, Inflammation and Lipid Metabolism’ EA7270/Inserm, 21000 Dijon; University of Burgundy, Center for Taste and Food Sciences, CNRS, INRAE, Institut Agro, Dijon, 21000 (FR)
  • Abdelkader AOUES University of Oran 1 Ahmed Ben Bella, Faculty of Natural and Life Sciences, Department of Biology, Laboratory of Experimental Biotoxicology, Biodepollution and Phytoremediation, Oran 31100 (DZ)



aluminum, caffeic acid, coumarin, hepatoprotective, oxidative stress


Antioxidants and phenolic compounds have always been tested as chemoprotective agents to regulate disease progression related to oxidative stress. Through this study, we investigate the possible corrective effects of caffeic acid and coumarin against aluminum- (Al-) induced stress response and liver damage in rats. Male Wistar rats were divided into four groups (10 rats per group), cotreated with caffeic acid (30 mg kg-1) and coumarin (5 mg kg-1) and exposed to aluminum (60 mg kg-1) for 45 days. Al induced significant alterations in body and liver weight over time. It also led to a marked increase in plasma levels of transaminases (aspartate aminotransferase and alanine aminotransferase), alkaline phosphatase, and lactate dehydrogenase. Additionally, there was a substantial change in the lipid profile, characterized by elevated levels of triglycerides and total cholesterol in both serum and liver. Furthermore, the LDL-c level and the HDL-c/total cholesterol ratio in serum were also affected. In contrast, the levels of HDL-c in serum and phospholipids in liver tissues were reduced. The results of this study also showed increased levels of TBARS, protein carbonyls, nitrates/nitrites, and TNF-α. Conversely, the activity of antioxidant enzymes (catalase, GP-x, and SOD) was reduced. These altered parameters were restored after treating rats with caffeic acid and coumarin. Our study strongly suggests that caffeic acid and coumarin possess antioxidant properties and hepatoprotective capacity by attenuating aluminum toxicity-induced liver damage.


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How to Cite

CHOUARI, Z., KHAROUBI, O., KESSAS, K., LOUNIS, W., VEJUX, A., & AOUES, A. (2024). Therapeutic potential of caffeic acid and coumarin in modifying aluminum-induced hepatic injury in Wistar rats. Notulae Scientia Biologicae, 16(1), 11748.



Research articles
DOI: 10.55779/nsb16111748