Antifungal activity against Candida albicans biofilm of Coffea robusta monofloral honey from Vietnam

Quoc-Dat DANG; Thuy-Trinh T. NGUYEN; Chau D. BUI; Hieu TRAN-VAN

doi:10.55779/nsb16111600

Authors

Quoc-Dat DANG University of Science, Faculty of Biology and Biotechnology, Department of Molecular and Environmental Biotechnology, Laboratory of Biosensors, 227 Nguyen Van Cu, Ho Chi Minh City 700000; University of Science, Laboratory of Molecular Biotechnology, 227 Nguyen Van Cu, Ho Chi Minh City 700000; Vietnam National University, Quarter 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam 700000 (VN)
Thuy-Trinh T. NGUYEN University of Science, Faculty of Biology and Biotechnology, Department of Molecular and Environmental Biotechnology, Laboratory of Biosensors, 227 Nguyen Van Cu, Ho Chi Minh City 700000; University of Science, Laboratory of Molecular Biotechnology, 227 Nguyen Van Cu, Ho Chi Minh City 700000; Buon Ma Thuot Medical University, Faculty of Medicine, Department of Biochemistry, 298 Ha Huy Tap, Buon Ma Thuot, Dak Lak Province 630000 (VN)
Chau D. BUI University of Science, Faculty of Biology and Biotechnology, Department of Molecular and Environmental Biotechnology, Laboratory of Biosensors, 227 Nguyen Van Cu, Ho Chi Minh City 700000; University of Science, Laboratory of Molecular Biotechnology, 227 Nguyen Van Cu, Ho Chi Minh City 700000; Vietnam National University, Quarter 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam 700000 (VN)
Hieu TRAN-VAN University of Science, Faculty of Biology and Biotechnology, Department of Molecular and Environmental Biotechnology, Laboratory of Biosensors, 227 Nguyen Van Cu, Ho Chi Minh City 700000; University of Science, Laboratory of Molecular Biotechnology, 227 Nguyen Van Cu, Ho Chi Minh City 700000; Vietnam National University, Quarter 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam 700000 (VN)

DOI:

https://doi.org/10.55779/nsb16111600

Keywords:

antifungal activity, biofilm, Candida albicans, honey, minimum inhibitory concentration (MIC), transcription level

Abstract

Coffee (Coffea robusta) flower honey is one of the highest-yielding kinds of honey in Vietnam. Nevertheless, there is little research on the antifungal activity associated with this type of honey. Therefore, this study was conducted to investigate the antifungal activity against C. albicans of Vietnamese coffee monofloral honey and its potential effects on biofilm formation. This study was conducted using the broth microdilution method. The results showed that coffee flower honey was resistant to Candida albicans strain with minimum inhibitory concentration (MIC) and with minimum fungicidal concentration (MFC) values of 50% and 70% (w/v), respectively. No change in the antifungal activity of honey and the antifungal agent (Amphotericin B) against the C. albicans strain occurred after five consecutive exposures. When using SEM, honey at MIC (w/v) strongly affected the two stages of C. albicans biofilm formation. The surface structure of the biofilm, deformed, distorted, and atrophied phenotypes impacted by honey were also observed. Expression of transcriptional-level genes involved in biofilm formation, quorum-sensing, and stress survival was analyzed by RT-qPCR in honey-treated and untreated biofilms. The present study showed the antifungal activity of Vietnamese coffee flower honey against C. albicans.

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