Biochemical composition and antioxidant activity of the mannoprotein preparation obtained yeast biomass from wine industry waste
Keywords:antioxidant and enzymatic activities, carbohydrates, mannoprotein preparation, proteins, waste, yeasts
In this study, the procedure of obtaining new biologically active mannoprotein preparation based on the yeast biomass from the residues of the wine industry, namely the production of Merlot dry red wine is presented. The process for obtaining of the preparation includes lysis of the yeast cell wall with sodium phosphate buffer, extraction of anthocyanins by treatment with 50% alcohol solution, hydrolysis with 1N NaOH, sedimentation of mannoproteins with ethyl alcohol, centrifugation and dissolution of purified sediment in distilled water, standardization of the preparation obtained up to a concentration of 10 mg/ml. As a result, it has been established that the mannoprotein preparation possesses a valuable biochemical composition characterized by a high content of proteins, carbohydrates, anthocyanins, and a wide range of minerals, macro, microelements, and does not practically contain heavy metals. Due to its biochemical composition, the preparation, also, possessed antioxidant activities and high catalase and superoxide dismutase activity. In conclusion, we can mention that the varied biochemical composition, antioxidant, and enzymatic activity highlight the high biological value of the mannoprotein preparation and the enormous potential for implementation in various fields, especially in animal husbandry and viticulture, food and cosmetic industry.
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