In vitro clonal propagation and evaluation of antibacterial activity of Benghal dayflower-Commelina benghalensis L.

  • Md. M. ISLAM Plant Biotechnology and Genetic Engineering Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Dhaka-1000
  • Md. A. AL MAMUN Agricultural Statistics Division, Bangladesh Rice Research Institute, Gazipur
  • Md. F. ALAM University of Rajshahi, Department of Botany, Rajshahi
Keywords: bacterial strain; Benghal dayflower; nodal segment; organic solvent; plant extracts


An efficient protocol for in vitro propagation of Commelina benghalensis was developed. Nodal segments were showed the superb explants in performance for shoot proliferation than other explants. On the other hand, BAP with auxin was better combination. The maximum (90.52) number of explants response and the highest (23.25) number of shoots per plant were obtained from nodal segments on MS medium fortified with 3.0 mg/l BAP+0.1 mg/l IBA. The highest shoot length (17.25 cm) was achieved on MS medium containing 3.0mg/l BAP+0.5mg/l IBA. In vitro proliferated shoots were transferred to full and half strength of MS media where 1.5 mg/l IBA on full strength of MS media was the best to fit for the maximum number (12.69) of roots formation per micro-shoot. Well rooted plantlets were transferred to soil and successfully acclimatized with 97% survival rate. Three extracts i.e. methanol, ethanol, Petroleum ether of C. benghalensis L and four concentrations of each extracts were used against five gram (+ve) and five gram (-ve) bacterial species for the screening of antibacterial activity. Ethanol extracts was the superior in performance. The susceptibility of tested pathogenic bacterial species was increasing compare with increasing of extracts concentration with few exceptions. The highest zone of inhibition was obtained against S. aureus (17.50 mm) and P. aeruginosa (17.44 mm) at 800 mg/l dose level of extracts. It was also noticed that Gram (+ve) bacterial species are more susceptible to Benghal dayflower crude extracts than Gram (-ve) bacterial species.


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Aftab K, Sial AA (1999). Phytomedicine: New and old approach. Hamdard Medicus 42(2):11-15.

Anand SP, Jeyachandran R (2004). In vitro multiple shoot regeneration from nodal explants of Zehneria scabra (L.f.) Sonder - an important medicinal climber. Plant Tissue Culture 14(2):101-106.

Bauer AW, Kirby WMW, Sherris JC, Turck M (1966). Antibiotic susceptibility testing by a standardized single disc method. American Journal of Clinical Pathology 45:494-496.

Borthakur M, Dutta K, Nath SC, Singh RS (2000). Micropropagation of Eclipta alba and Eupatorium adenophorum using a single step nodal cutting technique. Plant Cell, Tissue and Organ Culture 62:239-242.

Boro P, Sarma S, Deka AC, Kalita MC (1998). Clonal propagation of Alternanthera sessilis- A bio-pharmaceutically potent herbal medicinal plant. Journal of Phytological Research 11(2):103-106.

Ghani A (2003). Medicinal plants of Bangladesh: chemical constituents and uses. Asiatic Society of Bangladesh, Dhaka, Bangladesh pp 267.

Hamill FA, Mubiru NK, Mosango M, Bukenya-Ziraba R, Maganyi OW, Soejarto DD (2003). Traditional herbal drugs of southern Uganda II. Literature analysis and antimicrobial assays. Journal of Ethnopharmacology 84:57-78.

Hong D, Defillipps RA (2000). Commelina diffusa. In: Wu ZY, Raven PH, Hong DY (Eds.), Flora of China. Beijing Science Press; St Louis: Missouri Botanical Garden Press pp 86.

Jigna P, Sumitra C (2006). In vitro antimicrobial activities of extracts of Launaea procumbns Roxb. (Labiateae), Vitis vinifera L. (Vitaceae) and Cyperus rotundus L. (Cyperaceae). African Journal of Biomedical Research 9(2):89-93.

Manandhar N, Sanjay P (2000). Plants and people of Nepal. Timber press. Nepal.

Murashige T, Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiology 15:473-497.

Rabe T, Van Staden J (1997). Antibacterial activity of South African plants used for medicinal purposes. Journal of Ethnopharmacology 56:81-87.

Sarkar AKA, Kumar NK, Dutta P, Chakraborty P, Dastidar SG (2003). Evaluation of in vitro and in vivo antibacterial activity of dobutamine hydrochloride. The Indian Journal Medical Microbiology 21(3):172-178.

Sarwar S, Zia M, Rehman RU, Fatima Z, Sial RA, Chaudhary MF (2009). In vitro direct regeneration in mint from different explants on half strength MS medium. African Journal of Biotechnology 8(18):4667-4671.

Seetharam YN, Barad A, Chalegeri G, Jyothishwaran G, Ghanti KS, Bhakri V (2002). In vitro shoot regeneration from leaf and nodal explants of Enicostemma hyssopifolium (Willd.) Verd. - a vulnerable medicinal plant. Indian Journal of Biotechnology 1:401-404.

Taylor JLS, Rabe T, McGraw LJ, Jager AK, van Staden J (2001). Towards the scientific validation of traditional medicinal plants. Plant Growth Regulation 34:23-37.

Vlietinck AJ, Vanden BPA (1991). Can ethno-pharmacology contribute to the development of antiviral drugs? Journal of Ethnopharmacology 32(1-3):141-153.

How to Cite
ISLAM, M. M., AL MAMUN, M. A., & ALAM, M. F. (2020). In vitro clonal propagation and evaluation of antibacterial activity of Benghal dayflower-Commelina benghalensis L. Notulae Scientia Biologicae, 12(3), 628-636.
Research articles