Enhancement of the soil quality of an oil-polluted ultisol using livestock wastes


  • Sylvia O. OGOANAH University of Benin, Department of Animal and Environmental Biology (NG)
  • Uzoamaka N. NGWOKE University of Benin, Department of Animal and Environmental Biology; University of Benin, Department of Plant Biology and Biotechnology, Environmental Biotechnology and Sustainability Research Group (NG)
  • Edokpolor O. OHANMU University of Benin, Department of Plant Biology and Biotechnology, Environmental Biotechnology and Sustainability Research Group; Edo University Iyamho, Department of Biological Sciences, Edo State (NG)
  • Pascal C. OKOYE University of Benin, Department of Plant Biology and Biotechnology, Environmental Biotechnology and Sustainability Research Group (NG)
  • Beckley IKHAJIAGBE University of Benin, Department of Plant Biology and Biotechnology, Environmental Biotechnology and Sustainability Research Group; University of Benin, Department of Microbiology, Applied Environmental Bioscience and Public Health Research Group, Benin City (NG)




bioremediation; pollution; remediation efficiency; soil amendment; ultisol; waste engine oil


The study investigated the enhancement of soil quality of an oil-polluted ultisol using livestock wastes. Top soil (0 - 10 cm) was obtained as a pooled sample and polluted with spent lubricating oil at 10% w/w. The soil was subsequently amended with sun-dried goat (GT), rabbit (RB), and poultry (PG) dung at 10% w/w on dry weight basis both in singles, double-mixed, and triple-mixed combinations. Twelve weeks after treatment application, results showed that there was a 93.9% decrease (p<0.05) in bacterial colony count in the oil-polluted soil compared to the control. Penicillium notatum and Aspergillus niger as well as Bacillus sp. and Proteus sp. were the prominent fungal and bacterial species identified respectively. The most abundant plant in the soil seed bank was Panicum maximum with 10.4% abundance and this showed possible involvement of the plant in remediation of oil-pollution. The total hydrocarbon content of the oil-polluted soil was 9984.0 mg/kg, compared to 3170.6 mg/kg when amended with RB+GT, implying 76.77% remediation efficiency. Among several trials employed in this study, the combination of rabbit and goat wastes proved to be more effective in reducing the total hydrocarbon content of oil-polluted soil and therefore, is recommended as a potential candidate for application in the bioremediation of such soil.


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Abosede EE (2013). Effect of crude oil pollution on some soil physical properties. Journal of Agriculture and Veterinary Science 6(3):14-17.

Achuba FI (2006). The effects of sublethal concentrations of crude oil on the growth and metabolism of cowpea (Vigna unguiculata) seedlings. Environment 26(1):17-20.

Adams GO, Tawari-Fufeyin P, Ehinomen I (2014). Bioremediation of spent oil contaminated soils using poultry litter. Research Journal in Engineering and Applied Sciences 3(2):124-130.

Adesodun JK, Mbagwu JSC (2008). Biodegradation of waste lubricating petroleum oil in a tropical soil as mediated by animal droppings. Bioresource Technology 99:5659-5665.

Agbogidi OM, Eruotor PG, Akparobi SO, Nnaji GU (2007). Evaluation of crude oil contaminated soil on the mineral elements of maize (Zea mays L). Journal Agronomy 6(1):188-193.

Akinde SB, Obire O (2008). Aerobic heterotrophic bacteria and petroleum-utilizing bacteria from cow dung and poultry manure. World Journal of Microbiology and Biotechnology 24(9):634-643.

Al-Qahtani MRA (2011). Effect of oil refinery sludge on plant growth and soil properties. Research Journal of Environmental Science 5(2):187-193.

Amadi A, UeBari Y (1992). Use of poultry manure for amendment of oil-polluted soils in relation to growth of maize (Zea mays L.). Environment International 18:521-527.

Anikwe MAN (2011). Low input agriculture technologies for sub-Saharan Africa. In: Frackson LM, Mkpado M (Eds). Low-Input Agriculture Technologies for Sub-Saharan Africa. Springer, Peter Lang, Internationaler Verlagder Wissenschaften, Frankfurt.

Anikwe MAN, Eze JC, Ibudialo AN (2016). Influence of lime and gypsum application on soil properties and yield of cassava (Manihot esculenta Crantz.) in a degraded Ultisol in Agbani, Enugu Southeastern Nigeria. Soil and Tillage Research 158:32-38.

Anoliefo GO, Ikhajiagbe B, Okoye PC, Osayi O (2016). Utilizing local soap-derived biosurfactant for degradation of petroleum hydrocarbon polluted soils: sustainable remediation in focus. Annals Science and Technology 1(1):43-51.

Bidwell JR, Donald SC, Merski T (2002). Toxicity evaluation of a commercial bioremediation agent mixed with crude oil. Environmental Toxicology and Chemistry 22(1):84-91.

Bonilla N, Gutiérrez-Barranquero JA, De Vicente A, Cazorla FM (2012). Enhancing soil quality and plant health through suppressive organic amendments. Diversity 4:475-491.

Boopathy R (2001). Factors limiting bioremediation technologies. Bioresource Technology 74:63-67.

Cheesbrough M (2004). District laboratory practice in tropical countries: Part 2. Cambridge University Press, Cambridge, UK. pp 299-329.

Chikere CB, Chikere BO, Okpokwasili GC (2012). Bioreactor-based bioremediation of hydrocarbon polluted Niger Delta Marine Sediment. Nigerian Biotechnology 2(1):53-66.

Cowan ST, Steel KJ (1965). Manual for the Identification of Medical Bacteria. Cambridge University Press, Cambridge, UK.

Ekpo MA, Ebeagwu CJ (2009). The effect of crude oil on microorganisms and dry matter of fluted pumpkin (Telfairia occidentalis). Scientific Research and Essay 4(8):733-739.

Ekpo MA, Nwaankpa IL (2005). Effect of crude oil on microorganisms and growth of ginger (Zingiber officinale) in the tropics. Journal of Sustainable Tropical Agricultural Research 16:67-71.

Ekundayo EO, Emede TO, Osayande DI (2001). Effects of crude oil spillage on growth and yield of maize (Zea mays L.) in soil of Midwestern Nigeria. Plant Foods for Human Nutrition 56(4):313324.

Ewulo BS (2005). Effect of poultry dung and cattle manure on chemical properties of clay and sandy clay loam soil. Journal of Animal and Veterinary Advances 4(10):839-841.

Eziuzor CS, Okpokwasili GC (2009). Bioremediation of hydrocarbon contaminated mangrove soil in a bioreactor. Nigerian Journal of Microbiology 23(1):1777-1791.

Farhana A, Guidry L, Srivastava A, Singh A, Hondalus MK, Steyn JCA (2010). Reductive Stress in Microbes: Implications for understanding Mycobacterium tuberculosis disease and persistence. Advances in Microbial Physiology 57:543-552.

Gupta G, Baummer J (1996). Biodegradation of atrazine in soil using poultry litter. Journal of Hazardous Materials 45:185-192.

Gupta G, Tao J (1996). Bioremediation of gasoline-contaminated soil using poultry litter. Journal of Environment Science and Health 31(9):2395-2407.

Ijah UJJ, Antai SP (2003). Removal of Nigerian light crude oil soil over a 12-month period. International Biodeterioration and Biodegradation 51(2):93-99.

Ikhajiagbe B, Anoliefo GO (2011). Natural attenuation of a 14- month-old spent engine oil-polluted soil. Journal Soil Science and Environmental Management 2(7):184-192.

Ikpe FN, Powel JM (2002). Nutrient cycling practices and changes in soil properties in the crop - livestock farming systems of western Niger Republic of West Africa. Nutrient Cycling in Agroecosystem 62:37-45.

Ikuesan FA, Boboye BE, Adetuyi FC (2016). Comparative bioremediation of crude oil – contaminated soil samples using activated soil and activated cow dung. Sky Journal of Microbiology Research 4(4):21-30.

Leahy JG, Colwell RR (1990). Microbial degradation of hydrocarbons in the environment. Microbiology Reviews 54:305-315.

Llado S, Solanas AM, De Lapuente J, Borras M, Vinas M (2012). A diversified approach to evaluate biostimulation and bioaugmentation strategies for heavy-oil-contaminated soil. Science of the Total Environment 436:262-269.

Mishra S, Sarma PM, Lal B (2004). Crude oil degradation efficiency of a recombinant Acinetobacter baumannii strain and its survival in crude oil-contaminated soil microcosm. FEMS Microbiology Letters 235(2):323-331.

Mukala RA, Lockwood PJ, Finnerty WR (1975). Comparative analysis of the lipids of Acinetobacter species grown on hexadecane. Journal of Bacteriology 12(1):250-258.

Nwogu TP, Azubuike CC, Ogugbue CJ (2015). Enhanced bioremediation of soil artificially contaminated with petroleum hydrocarbons after amendment with Capra aegagrus hircus (Goat) manure. Biotechnology Research International 657349. https://doi.org/10.1155/2015/657349

Obiakalaije UM, Makinde OA, Amakoromo ER (2015). Bioremediation of crude oil-polluted soil using animal waste. International Journal of Environmental Bioremediation and Biodegradation 3(3):79-85.

Obire O, Anyanwu EC (2009). Impact of various concentrations of crude oil on fungal populations of soil. International Journal of Environmental Science Technology 6(2):211-218.

Ogboghodo I, Erebor E, Osemwota I, Isitekale H (2004). The effects of application of poultry manure to crude oil-polluted soils on maize growth and soil properties. Environmental Monitoring Assessment 96:153-161.

Ohanmu EO, Bako SP (2017). Reproductive capacity of Capsicum sp. as affected by crude oil pollution in two weather conditions. Research and Reviews: Research Journal Biology 5(4):8-12.

Ohanmu EO, Bako SP, Adelanwa MA (2014). Effect of crude oil polluted soil on the growth and survival of pepper (Capsicum annuum L.). Annals Experimental Biology 2(4):5-10.

Ohanmu EO, Ikhajiagbe B (2018). Effect of cadmium pollution on nitrogen assimilation and biomass accumulation of Vigna unguiculata L. Asian Journal of Applied Sciences 11:183-191.

Okieimen CO, Okieimen FE (2005). Bioremediation of crude oil-polluted soil–effect of poultry droppings and natural rubber processing sludge application on biodegradation of petroleum hydrocarbons. Environmental Sciences 12(1):1-8.

Okolo JC, Amadi EN, Odu CTI (2005). Effects of soil treatment containing poultry manure on crude oil degradation in sandy loam soil. Applied Ecology and Environmental Research 3(1):47-53.

Orji FA, Ibiene AA, Dike EN (2012). Laboratory scale bioremediation of petroleum hydrocarbon-polluted mangrove swamp in the Niger Delta using cow dung. Malaysian Journal of Microbiology 8(4):219-228.

Paiga P, Mendes L, Albergaria JT, Delerue-Matos CM (2012). Determination of total hydrocarbons in soil from different locations using infrared spectrophotometry and gas chromatography. Chemical Papers 66(8):711-721.

Powel JM, Ikpe FN, Somala ZC, Rivera SF (1998). Urine effects on soil chemical properties and the impact of urine and dung on pearl millet yield. Experimental Agriculture 34:250-279.

Saroj A, Keerti D (2013). Isolation and characterization of hydrocarbon degrading microorganisms from petroleum oil contaminated soil sites. Bulletin of Environmental and Scientific Research 2(4):5-10.

Shabir GM, Afzal M, Anwar F, Tahseen R, Khalid ZM (2008). Biodegradation of kerosene in soil by a mixed bacterial culture under different nutrient conditions. International Biodeterioration and Biodegradation 61(2):61-166.

Sutton NB, Maphosa F, Morillo JA (2013). Impact of long-term diesel contamination on soil microbial community structure. Applied and Environmental Microbiology 79(2):619-630.

Tuyen PT, Xuan TD, Tuanh TT, Van TM, Ahmad A, Elzaawely AA, Khanh TD (2018). Weed Suppressing Potential and Isolation of Potent Plant Growth Inhibitors from Castanea crenata Sieb. et Zucc. Molecules 23(345): doi:10.3390/molecules23020345.

Wyszkowski M, Ziolkowska A (2008). Effect of petrol and diesel oil on content of organic carbon and mineral components in soil. American-Eurasian Journal Sustainable Agriculture 2(1):54-60.




How to Cite

OGOANAH, S. O., NGWOKE , U. N., OHANMU , E. O. ., OKOYE, P. C., & IKHAJIAGBE, B. (2020). Enhancement of the soil quality of an oil-polluted ultisol using livestock wastes. Notulae Scientia Biologicae, 12(2), 387–398. https://doi.org/10.15835/nsb12210617



Research articles
DOI: 10.15835/nsb12210617

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