Differential Activity of Antioxidant Enzymes and Physiological Changes in Wheat (Triticum aestivum L.) Under Drought Stress
DOI:
https://doi.org/10.15835/nsb11210390Keywords:
antioxidant enzymes; drought stress; grain yield; landraces; wheatAbstract
Drought stress is one of the most significant environmental factors restricting plant production all over the world. In arid and semi-arid regions where drought often causes serious problems, wheat is usually grown as a major crop and faces water stress. In order to study drought tolerance of wheat, an experiment with 34 genotypes including 11 local and commercial cultivars, 17 landraces, and six genotypes from International Maize and Wheat Improvement Center (CIMMYT) was conducted at the experimental station, School of Agriculture, Shiraz University, Iran in 2010-2011 growing season. Three different irrigation regimes (100%, 75% and 50% Field Capacity) were applied and physiological and biochemical traits were measured for which a significant difference was observed in genotypes. Under severe water stress, proline content and enzymes’ activities increased while the relative water content (RWC) and chlorophyll index decreased significantly in all genotypes. Of these indices, superoxide dismutase (SOD) and RWC were able to distinguish tolerant genotypes from sensitives. Moreover, yield index (YI) was useful in detecting tolerant genotypes. The drought susceptibility index (DSI) varied from 0.40 to 1.71 in genotypes. These results indicated that drought-tolerant genotypes could be selected based on high YI, RWC and SOD and low DSI. On the whole, the genotypes 31 (30ESWYT200), 29 (30ESWYT173) and 25 (Akbari) were identified to be tolerant and could be further used in downstream breeding programs for the improvement of wheat tolerance under water limited conditions.
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Almeselmani M, Abdullah F, Hareri F, Naaesan M, Ammar MA, Kanbar OZ, Saud AA (2011). Effect of drought on different physiological characters and yield component in different varieties of Syrian durum wheat. Journal of Agricultural Science 3(3):127-133.
Almeselmani M, Deshmukh PS, Sairam RK, Kushwaha SR, Singh TP (2006). Protective role of antioxidant enzymes under high temperature stress. Plant Science 171(3):382-388.
Apel K, Hirt H (2004). Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annual Review of Plant Biology 55:373-399.
Asada K (1992). Ascorbate peroxidase-a hydrogen peroxide-scavenging enzyme in plants. Physiologia Plantarum 85(2):235-241.
Asada K, Takahashi M (2006). Production and scavenging of active oxygen in photosynthesis. Physiologia Plantarum 141:391-405.
Bartosz G (1997). Oxidative stress in plants. Acta Physiologia Plantrum 19(1):47-64.
Bates LS, Waldren RP, Teare ID (1973). Rapid determination of free proline for water-stress studies. Plant and Soil 39(1):205-207.
Beauchamp C, Fridovich I (1971). Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry 44(1):276-287.
Bouslama M, Schapaugh WT (1984). Stress tolerance in soybeans. I. Evaluation of three screening techniques for heat and drought tolerance 1. Crop Science 24(5):933-937.
Castrillo M, Calcagno AM (1989). Effects of water stress and re watering on ribulose-1, 5-bis-phosphate carboxylase activity, chlorophyll and protein contents in two cultivars of tomato. Journal of Horticultural Science 64(6):717-724.
Chance B, Maehly AC (1995). Assay of catalase and peroxidase. Journal of Plant Physiology 2:764-791.
DaCosta M, Huang B (2007). Changes in antioxidant enzyme activities and lipid peroxidation for bentgrass species in response to drought stress. Journal of the American Society for Horticultural Science 132(3):319-326.
De Carvalho MHC (2008). Drought stress and reactive oxygen species: production, scavenging and signaling. Plant Signaling and Behavior 3:156-165.
Devi R, Kaur N, Gupta, AK (2012). Potential of antioxidant enzymes in depicting drought tolerance of wheat (Triticum aestivum L.). Indian Journal of Biochemistry and Biophysics 49:257-265.
Dorostkar S, Dadkhodaie A, Heidari B (2015). Evaluation of grain yield indices in hexaploid wheat genotypes in response to drought stress. Archives of Agronomy and Soil Science 61(3):397-413.
Dorostkar S, Dadkhodaie A, Heidari B (2016). Changes in biochemical characteristics of thirty-six Iranian wheat landraces in response to drought stress and their classification using multivariate analysis. Journal of Animal and Plant Science 26(6):1740-1749.
Ehdaie B, Alloush GA, Waines JG (2008). Genotypic variation in linear rate of grain growth and contribution of stem reserves to grain yield in wheat. Field Crops Research 106(1):34-43.
Emam Y, Nik Nejad M (1994). An introduction to physiology of crop yield. Shiraz, Shiraz University Press [in Persian].
FAO STAT (2017). http://faostat.fao.org/site/339/default.aspx.
Fischer RA, Maurer R (1978). Drought resistance in spring wheat cultivars. I. Grain yield responses. Australian Journal of Agricultural Research 29(5):897-912.
González A, MartıÌn I, Ayerbe L (1999). Barley yield in water-stress conditions: The influence of precocity, osmotic adjustment and stomatal conductance. Field Crops Research 62(1):23-34.
Guttieri MJ, Stark JC, O'Brien K, Souza E (2001). Relative sensitivity of spring wheat grain yield and quality parameters to moisture deficit. Crop Science 41(2):327-335.
Hall AJ, Richards RA (2013). Prognosis for genetic improvement of yield potential and water-limited yield of major grain crops. Field Crops Research 143:18-33.
Hendry GAF, Price AH (1993). Stress indicators: chlorophylls and carotenoids. Chapman & Hall, London.
Keyvan S (2010). The effects of drought stress on yield, relative water content, proline, soluble carbohydrates and chlorophyll of bread wheat cultivars. Journal of Animal and Plant Sciences 8(3):1051-1060.
Khanna-Chopra R, Selote DS (2007). Acclimation to drought stress generates oxidative stress tolerance in drought-resistant than-susceptible wheat cultivar under field conditions. Environmental and Experimental Botany 60(2):276-283.
Kirigwi FM, van Ginkel M, Trethowan R, Sears RG, Rajaram S, Paulsen GM (2004). Evaluation of selection strategies for wheat adaptation across water regimes. Euphytica 135(3):361-371.
Lichtenthaler H, Wellburn AR (1983). Determination of total carotenoids and chlorophyll a and chlorophyll b leaf extracts in different solvents. Biochemical Society Transactions 11:591-592.
Liu X, Huang B (2000). Heat stress injury in relation to membrane lipid peroxidation in creeping bentgrass. Crop Science 40(2):503-510.
Mercado JA, Matas AJ, Heredia A, Valpuesta V, Quesada MA (2004). Changes in the water binding characteristics of the cell walls from transgenic Nicotiana tabacum leaves with enhanced levels of peroxidase activity. Physiologia Plantarum 122(4):504-512.
Mitra J (2001). Genetics and genetic improvement of drought resistance in crop plants. Current Science 80:758-763.
Mittler R (2006). Abiotic stress, the field environment and stress combination. Trends in Plant Science 11(1):15-19.
Nyachiro JM, Briggs KG, Hoddinott J, Johnson-Flanagan AM (2001). Chlorophyll content, chlorophyll fluorescence and water deficit in spring wheat. Cereal Research Communications 29:135-142.
Passioura J (2007). The drought environment: physical, biological and agricultural perspectives. Journal of Experimental Botany 58(2):113-117.
Pireivatloum J, Qasimov N, Maralian H (2010). Effect of soil water stress on yield and proline content of four wheat lines. African Journal of Biotechnology 9(1):36-40.
Pourtaghi A, Darvish F, Habibi D, Nourmohammadi G, Daneshian J (2011). Effect of irrigation water deficit on antioxidant activity and yield of some sunflower hybrids. Australian Journal of Crop Science 5(2):197-204.
Rao L, Perez D, White E (1996). Lamin proteolysis facilitates nuclear events during apoptosis. Journal of Cell Biology 135(6):1441-1455.
Saini HS, Westgate ME (2000). Reproductive development in grain crops during drought. Advances in Agronomy 68:59-95.
Sairam RK, Deshmukh PS, Shukla DS (1997). Tolerance of drought and temperature stress in relation to increased antioxidant enzyme activity in wheat. Journal of Agronomy and Crop Science 178(3):171-178.
Sairam RK, Saxena DC (2000). Oxidative stress and antioxidants in wheat genotypes: possible mechanism of water stress tolerance. Journal of Agronomy and Crop Science 184(1):55-61.
Sairam RK, Srivastava GC (2001). Water stress tolerance of wheat (Triticum aestivum L.): variations in hydrogen peroxide accumulation and antioxidant activity in tolerant and susceptible genotypes. Journal of Agronomy and Crop Science 186(1):63-70.
Sarvajeet SG, Narendra T (2010). Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry 48(2):909-930.
SAS (2004). SAS version 9.2. Cary (NC): SAS Institute Inc. SAS Online Doc 913.
Schonfeld M, Johnson R, Carver B, Mornhinweg D (1988). Water relations in winter wheat as drought resistant indicators. Crop Science 28(3):526-531.
Shao HB, Liang ZS, Shao MA, Sun Q (2005). Dynamic changes of anti-oxidative enzymes of 10 wheat genotypes at soil water deficits. Colloids and Surfaces B: Biointerfaces 42(3-4):187-195.
Sheoran S, Pandey B, Sharma P, Narwal S, Singh R, Sharma I, Chatrath R (2013). In silico comparative analysis and expression profile of antioxidant proteins in plants. Genetics and Molecular Research 12(1):537-551.
Sheoran S, Thakur V, Narwal S, Turan R, Mamrutha HM, Singh V, Sharma I (2015). Differential activity and expression profile of antioxidant enzymes and physiological changes in wheat (Triticum aestivum L.) under drought. Applied Biochemistry and Biotechnology 177(6):1282-1298.
Shimshi D, Mayoral M, Atsmon D (1982). Responses to water stress in wheat and related wild species. Crop Science 22(1):123-128.
Shinozaki K, Yamaguchi-Shinozaki K (2007). Gene networks involved in drought stress response and tolerance. Journal of Experimental Botany 58(2):221-227.
Sio-se Mardeh A, Ahmadi A, Poustini K, Mohammadi V (2006). Evaluation of drought resistance indices under various environmental conditions. Field Crops Research 98(2-3):222-229.
Valifard M, Moradshahi A, Kholdebarin B (2012). Biochemical and physiological responses of two wheat (Triticum aestivum L.) cultivars to drought stress applied at seedling stage. Journal of Agriculture Science and Technology 14:1567-1578.
Wang HZ, Zhang LH, Jun MA, Li XY, Yan LI, Zhang RP, Wang RQ (2010). Effects of water stress on reactive oxygen species generation and protection system in rice during grain-filling stage. Agricultural Sciences in China 9(5):633-641.
Zhang J, Kirkham MB (1994). Drought stress-induced changes in activities of superoxide dismutase, catalase and peroxidase in wheat species. Plant and Cell Physiology 35(5):785-791.
Zimmerman JD (2002). Irrigation. New York, John Wiley & Sons Inc.
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