TY - JOUR AU - MONDAL, Rajendra P. AU - GHOSH, Anupam AU - BURMAN, Sunanda AU - CHANDRA, Goutam PY - 2022/06/14 Y2 - 2024/03/29 TI - Efficacy of ethyl acetate extract of Alangium salviifolium fruit pericarp against Culex quinquefasciatus larvae JF - Notulae Scientia Biologicae JA - Not Sci Biol VL - 14 IS - 2 SE - Research articles DO - 10.55779/nsb14211251 UR - https://notulaebiologicae.ro/index.php/nsb/article/view/11251 SP - 11251 AB - <p>Mosquitoes transmit major human diseases, resulting in millions of fatalities each year and the development of chemical insecticide resistance, leading to a rebound in vectorial capacity. Plants could be used as a mosquito repellent alternative. The purpose of this study was to evaluate the biocontrol potentiality of ethyl acetate extract of fruit pericarp of <em>Alangium salviifolium </em>against <em>Culex quinquefasciatus </em>larvae. 100% larval mortality was recorded after 72 h of exposure with 50, 40 ppm, and 30 ppm concentrations of ethyl acetate extract against 3<sup>rd </sup>instar mosquito larvae. The bioactive compound responsible for larval mortality was isolated by TLC (R<sub>f</sub> value of 0.33). 3<sup>rd</sup> instar larvae were found to be the most susceptible (LC<sub>50</sub> = 3.60 ppm) among all the instars and corresponding LC<sub>50</sub> values were 4.45 ppm and 4.52 ppm for 2<sup>nd</sup> and 4<sup>th </sup>instars larvae respectively after 72 h of exposure. The mortality rate of all larval instars was directly proportional to the concentration of bioactive compounds. Three-way ANOVA analysis revealed that larval instars, the concentration of bioactive compound, and time of exposure had a significant effect on larval mortality. In the bioactive TLC fraction of <em>A. salviifolium</em> (Rf value of 0.33), FT-IR spectroscopy analysis revealed the presence of numerous functional groups. GC-MS analysis revealed the presence of Benzoyl bromide and 3-Amino -5 (2-Furyl) Pyrazole in the extract. The compounds were also studied on non-target organisms such as <em>Anisops sardea</em>, 4<sup>th</sup> instar larvae of <em>Chironomus </em>sp. and <em>Diplonychus annulatum, </em>and in all the cases no abnormalities were recorded.</p> ER -