TY - JOUR AU - CHIPPY, Somarajan L. AU - ANJU VIJAY, Vijayakumar T. AU - HEMANTHAKUMAR, Achuthan S. AU - PILLAI, Padmesh P. AU - PREETHA, Thankappan S. PY - 2022/11/28 Y2 - 2024/03/28 TI - Enhanced production of lupeol through elicitation in in vitro shoot cultures of snake grass (Clinacanthus nutans) JF - Notulae Scientia Biologicae JA - Not Sci Biol VL - 14 IS - 4 SE - Research articles DO - 10.55779/nsb14411195 UR - https://notulaebiologicae.ro/index.php/nsb/article/view/11195 SP - 11195 AB - <p><em>Clinacanthus nutans</em>&nbsp;(Acanthaceae), generally known as ‘snake grass’, has diverse uses in customary system of herbal medicine. The species is endowed with various bioactive compounds exhibiting extensive pharmacological properties. The present investigation focused on elicitor-intervened <em>in vitro</em> shoot biomass cultivation and scale-up production of the anti-cancerous compound ‘lupeol’, one of the foremost constituents in this species. For the augmented production of lupeol, the shoot cultures were elicited with various concentrations of yeast extract (YE), chitosan and methyl jasmonate (MeJA). Maximum shoot biomass yield and production of lupeol was detected in MS medium supplemented with 1.0 mgl<sup>-1</sup> BA and 400 mgl<sup>-1</sup> YE. The petroleum ether extracts of selected samples upon TLC analysis proved R<sub>f</sub> values corresponding to lupeol. HPTLC analysis revealed that the sample treated with YE displayed relatively higher amount (975.50 ng) of lupeol than the <em>in vivo </em>plant (713.69 ng). Hence the <em>in vitro</em> shoot culture system with elicitor (YE) treatment propose an appropriate method for the elevated synthesis of lupeol which can be scaled up <em>via</em> bio-reactor technology in doing so profiting the pharmaceutical appliances.</p> ER -