TY - JOUR AU - AKINROPO, Michael S. AU - AYISIRE, Benjamin E. AU - OGBIMI, Ejeoghene R. PY - 2020/03/31 Y2 - 2024/03/29 TI - In vitro callus and shoot regeneration in Enterolobium cyclocarpum (Jacq.) Grised. - a fast timber yielding species JF - Notulae Scientia Biologicae JA - Not Sci Biol VL - 12 IS - 1 SE - Research articles DO - 10.15835/nsb12110604 UR - https://notulaebiologicae.ro/index.php/nsb/article/view/10604 SP - 74-89 AB - <p style="text-align: justify;">This study was conducted to investigate the <em>in vitro</em> callus induction and rapid shoot regeneration potential in<em> Enterolobium cyclocarpum</em>, a plant native to central Mexico but widely introduced into Africa. The leaf, stem and nodal explants of <em>E. cyclocarpum </em>were cultured on full strength Murashige and Skoog (MS) medium supplemented with different concentrations of Cytokinins - Benzyladenine (BA) and/or Kinetin and Auxins - Naphthalene acetic acid (NAA) and/or 2,4-Dichlorophenoxylacetic acid (2,4-D) each alone and in combination.&nbsp; The leaf explants did not respond to these treatments.&nbsp; The Nodal explants were best for caulogenesis, while the explant responses were in the order- nodal &gt; stem &gt; cotyledon for callogenesis in MS medium supplemented with BA and/or Kin combined with NAA and/or 2,4-D. The varied combinations induced white compact callus.&nbsp; The highest callus production was observed on MS medium supplemented with 2.7 μM NAA + 2.2 μM BA and 5.4 μM NAA alone.&nbsp; Nodal and cotyledon explants developed callus and multiple shoots on MS supplemented with a combination of cytokinin (BA and/or Kin.) and auxin (NAA and/or 2,4-D). The maximum number of 3.98 ± 0.37 and 2.1±0.11 shoots/explants were recorded for nodal and cotyledon explants on MS medium supplemented with a combination of 8.8 μM BA+2.7 μM NAA and 2.2μM BA+2.7 μM NAA respectively.&nbsp; On the basal medium, 10% of the excised shoots rooted successfully. Thus, this <em>in vitro</em> method can be exploited for conservation and mass propagation of this fast timber yielding tree and also utilized for embryogenesis studies.</p> ER -