TY - JOUR AU - CHUKWUNELO, Alpha C. AU - ANOSIKE, Assumpta C. AU - NNAMONU, Emmanuel I. AU - EKPO, Daniel E. AU - JAMES, Prince O. AU - OKONKWO, Tochukwu I. PY - 2019/12/24 Y2 - 2024/03/28 TI - Evaluation of Leukocyte Mobilization and Platelet Aggregatory Effects of Ciprofloxacin, Lincomycin and Erythromycin in Wistar Albino Rats JF - Notulae Scientia Biologicae JA - Not Sci Biol VL - 11 IS - 4 SE - Research articles DO - 10.15835/nsb11410491 UR - https://notulaebiologicae.ro/index.php/nsb/article/view/10491 SP - 345-351 AB - <p style="text-align: justify;">This study evaluated leucocyte mobilization and platelet aggregation effects of ciprofloxacin, lincomycin and Erythromycin in Wistar albino rats. Thirty-three female Wistar albino rats weighing 130-160 g and three male Wistar albino rats weighing 188-194 g, fed commercial growers’ mash and clean tap water were used. In leukocytes assay, thirty-three adult female Wistar rats were assigned into five treatments, eleven groups of three rats per group. First three groups were treated 10 mg kg<sup>-1</sup>, 20 mg kg<sup>-1</sup> and 40 mg kg<sup>-1</sup> of ciprofloxacin, next three groups same doses of lincomycin, next three groups same doses of Erythromycin, the tenth group received Indomethacin 5 mg kg<sup>-1</sup> (reference drug), the last group 5 mg kg<sup>-1 </sup>normal saline. Assay on platelet aggregator activity involved collection of blood samples (10 ml each, 1% EDTA, centrifuged at 3000 rpm for 10 minutes), test drugs dissolved in 1 mg/ml distilled water and&nbsp;&nbsp; Indomethacin (the reference drug). The three antibiotics significantly reduced leucocyte mobilization into the affected tissue. They all had their maximum inhibitory effects at the highest dose (40 mg kg<sup>-1</sup>) compared with indomethacin. Erythromycin 40 mg kg<sup>-1</sup> showed the highest inhibitory effect on leucocyte migration. Whereas ciprofloxacin and erythromycin had stepwise increase in absorbance from time 0 secs through to time 120 secs, lincomycin showed a sharp decrease in the absorbance at around 30 seconds followed by a continuous increase up to 120 seconds. The testing drugs prevented leukocyte mobilization also had stepwise increase in absorbance from time 0 secs through to time 120 secs in platelet aggregatory activity assay to some extent.</p> ER -