@article{KRUPA-MAŁKIEWICZ_Smolik_BARNIAK_SMOLIK_2014, title={RAPD and ISSR Methods Used for Fingerprinting of Selected Accessions of Viburnum}, volume={6}, url={https://notulaebiologicae.ro/index.php/nsb/article/view/9422}, DOI={10.15835/nsb639422}, abstractNote={<p style="text-align: justify;">Randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers were used to investigate genetic variability within thirteen <em>Viburnum</em> species (<em>Viburnum </em>× <em>hillieri; V. dilatatum</em>; <em>Viburnum </em>×<em> carlcephalum; V. opulus</em>;<em> V. hupehense</em>;<em> Viburnum</em>× <em>bodnantense; Viburnum </em>× <em>burkwoodii; V. sieboldii</em>; <em>Viburnum </em>× <em>globosum </em>‘Jermyns Globe’<em>; V. alnifolium (lantanoides); V. plicatum </em>‘Sterile’<em>;</em> <em>V. plicatum</em> f. <em>tomentosum</em> and<em> V. plicatum </em>‘Watanabe’) of wide geographical distribution, collected in the Dendrological Garden in Przelewice (the north-west part of Poland). Twenty-three RAPD and fourteen ISSR primers generated a total of 690 and 418 reproducible bands, respectively, and 39% (RAPD) and 55.5% (ISSR) of them were polymorphic for the two marker systems, which suggest high genetic variability within <em>Viburnum</em> genus. However, high numbers of genotype-specific bands, i.e. 60.9% (RAPD) and 44.5% (ISSR), were seen in <em>Viburnum</em>. Genetic similarity assessed within <em>Viburnum</em> species with the RAPD and ISSR analyses ranged from 6 to 42% and from 6 to 31%, respectively. Both RAPD and ISSR-based dendrograms clustered in five main groups. The Mantel test between two Nei’s similarity matrices gave correlation coefficient r<em>=</em>0.305*, showing low correlation between RAPD- and ISSR- based matrices. Thus, both marker systems were equally important for the genetic diversity analysis in <em>Viburnum</em> genus<em>.</em></p>}, number={3}, journal={Notulae Scientia Biologicae}, author={KRUPA-MAŁKIEWICZ, Marcelina and Smolik, Miłosz and BARNIAK, Anna and SMOLIK, Beata}, year={2014}, month={Sep.}, pages={292–299} }