Organic Additives Improves the in Vitro Growth of Native Orchid Vanda helvola Blume

  • Devina DAVID University Malaysia Sabah, Faculty of Sustainable Agriculture, Locked Bag No.3, 90509 Sandakan Sabah (MY)
  • Roslina JAWAN University Malaysia Sabah, Faculty of Science and Natural Resources, UMS Road, 88450 Kota Kinabalu Sabah (MY)
  • Hartinie MARBAWI University Malaysia Sabah, Faculty of Science and Natural Resources, UMS Road, 88450 Kota Kinabalu Sabah (MY)
  • Jualang Azlan GANSAU University Malaysia Sabah, Faculty of Science and Natural Resources, UMS Road, 88450 Kota Kinabalu Sabah (MY)
Keywords: basal media; conservation; in vitro seed germination; protocorm; Orchidaceae

Abstract

In vitro seed germination has been proven to be the most efficient technique to propagate orchid. The application of this aseptic technique has contributed to conservation of many endangered orchid species. In this study, undehisced capsules of Vanda helvola Blume were collected from Orchid Conservation Centre in Lagud Sebrang Agriculture Park, after 120 days from hand pollination and aseptically cultured on three types of basal media such as Murashige and Skoog (MS), Knudson C (KC) and Vacin and Went (VW). After 90 days of culture, 66.40 ± 4.14% of seeds successfully germinated on KC medium. The effect of organic additives such as tomato juice, coconut water, peptone and yeast extract at different level of concentrations in KC basal medium were also tested on seed germination and seedling development of this native orchid. After 90 days of culture, over 90% of seeds were tremendously germinated on KC medium supplemented with 10% or 15% (v/v) of tomato juice. The incorporation of peptone at 0.1% (w/v) in KC basal media promoted rapid development of protocorm to seedling. Seedlings on this treatment produced an average of three leaves and two roots after 90 days of culture and were successfully acclimatized.

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Published
2015-06-21
How to Cite
DAVID, D., JAWAN, R., MARBAWI, H., & GANSAU, J. A. (2015). Organic Additives Improves the in Vitro Growth of Native Orchid Vanda helvola Blume. Notulae Scientia Biologicae, 7(2), 192-197. https://doi.org/10.15835/nsb729546
Section
Research articles
CITATION
DOI: 10.15835/nsb729546