Preparation and Low Temperature Short-term Storage for Synthetic Seeds of Caladium bicolor

  • Mehpara MAQSOOD Cellular Differentiation and Molecular Genetics Section, Department of Botany, Hamdard University, New Delhi 110062
  • Abdul MUJIB Cellular Differentiation and Molecular Genetics Section, Department of Botany, Hamdard University, New Delhi 110062
  • Mir KHUSRAU Cellular Differentiation and Molecular Genetics Section, Department of Botany, Hamdard University, New Delhi 110062
Keywords: encapsulation; low temperature storage; ornamental specie; plant regeneration; somatic embryos

Abstract

An efficient somatic embryo encapsulation and in vitro plant regeneration technique were established with Caladium bicolor, an important ornamental plant.Tuber derived embryogenic callus (95.50%) was obtained on Murashige and Skoog (MS) medium amended with 0.5 mg L-1 α-Naphthalene acetic acid (NAA) + 0.5 mg L-1 6-Benzyladenine (BA). The embryogenic callus later differentiated into somatic embryos in the same plant growth regulators (PGRs) added medium (NAA and BA). The induced embryos matured and developed into plantlets in NAA and BA added media; maximum plantlets development was observed at 1.0 mg L-1 NAA + 1.0 mg L-1 BA supplemented medium. Synthetic seeds were produced by encapsulating embryos in gel containing 3.0% sucrose + 3.0% sodium alginate and 100 mM of calcium chloride.The highest synthetic seed germination (97.6%) was observed on medium supplemented with 1.0 mg L-1 NAA + 1.0 mg L-1 BA. The synthetic seeds were kept at low temperatures for storage; the encapsulated beads were viable and demonstrated good germination even after 12 weeks of storage at 4 °C. The plantlet recovery frequency was however declined with time. The synthetic seed derived plantlets were morphologically similar to the mother plant.

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Published
2015-03-20
How to Cite
MAQSOOD, M., MUJIB, A., & KHUSRAU, M. (2015). Preparation and Low Temperature Short-term Storage for Synthetic Seeds of Caladium bicolor. Notulae Scientia Biologicae, 7(1), 90-95. https://doi.org/10.15835/nsb719405
Section
Research article