Phenotypic aberrations during micropropagation of Soymida febrifuga (Roxb.) Adr. Juss

  • Kishore Kumar CHIRUVELLA Postdoctoral fellow, stockholm university
  • Arifullah MOHAMMED Division of Plant Tissue Culture, Department of Botany, Sri Venkateswara University, Tirupati, Andhra Pradesh Faculty of Agrobased Industry, Universiti Malaysia Kelantan, Jeli Campus, Locked bag-100, 17600, Jeli, Kelantan
  • Rama Gopal GHANTA Division of Plant Tissue Culture, Department of Botany, Sri Venkateswara University, Tirupati, Andhra Pradesh
Keywords: STN- shoot tip necrosis; CAP- calcium pantothenate; CAN- calcium nitrate; BA – 6; Benzyladenine; IBA - Indole 3 Butyric Acid; IAA – Indole 3 Acetic Acid; KN-kinetin; MS – Murashige and Skoog

Abstract

Like most of the medicinal plants Soymida febrifuga (Meliaceae) possess significance for its valuable secondary metabolites. Multiplication of this endemic plant is limited by difficulty in rooting of stem cuttings, high seedling mortality rates and low seed viability period. Hence efficient protocols for in vitro mass propagation has been established from field grown and aseptic seedlings explants. Strikingly, we observed aberrant structures such as vitrified shoots, faciated shoots, albino shoots as well shoot necrosis during its micropropagation. These phenotypic maladies were observed during organogenesis and rooting. Compared to other abnormalities, shoot necrosis nonetheless was frequent and pronounced leading to plant death. Shoots when subjected to rooting also displayed necrosis which was controlled by transferring to MS medium containing various concentrations and combinations of calcium levels, activated charcoal, glucose, fructose and auxins. Microshoots initiated roots on half strength MS medium with IBA and IAA individually or in combination within two weeks. MS half strength solid medium supplemented with CAN (556 mg l–1), CAP (1.0 mg l–1), IAA (2.0 mg l–1) and IBA (2.0 mg l–1) in combination was found to be more efficient in showing high frequency (95%) of root regeneration. Rooted plantlets were successfully hardened and 70-85% of regenerated plants were successfully acclimatized to natural environment. In vitro derived plantlets were morphologically similar to in vivo plants.

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Published
2014-03-12
How to Cite
CHIRUVELLA, K. K., MOHAMMED, A., & GHANTA, R. G. (2014). Phenotypic aberrations during micropropagation of Soymida febrifuga (Roxb.) Adr. Juss. Notulae Scientia Biologicae, 6(1), 99-104. https://doi.org/10.15835/nsb619202
Section
Research article

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