Morphogenetic Potential of Tomato (Lycopersicon esculentum) cv. ‘Arka Ahuti’ to Plant Growth Regulators

  • Kanakapura K. NAMITHA Fruit and Vegetable Technology Department, CSIR-Central Food Technological Research Institute, Mysore-570020 (IN)
  • Pradeep S. NEGI Fruit and Vegetable Technology Department, CSIR-Central Food Technological Research Institute, Mysore-570020 (IN)

Abstract

A highly reproducible in vitro regeneration method for tomato (Lycopersicon esculentum Mill.) cultivar ‘Arka Ahuti’ was established by using hypocotyl, leaf and cotyledon explants from in vitro raised seedlings on Murashige and Skoog medium supplemented with different concentrations and combinations of hormones 6-Benzylamino purine (2 to 4 mg/L) and Indole-3-acetic acid (0.1 to 1 mg/L). The medium supplemented with 2 mg/L 6-benzylamino purine and 0.1 mg/L indole-3-acetic acid was found to be the best for inducing direct shoot regeneration and multiple shoots per explant from hypocotyl explants. Callus induction was observed in all the explants and regeneration of shoots was also promoted by all these combinations. Shoots were transferred to the elongation medium which also induced 100% rooting. After hardening, plants were transferred to soil. Thus, a tissue culture base line was established for ‘Arka Ahuti’ cultivar of tomato for obtaining direct regeneration using hypocotyl, leaf and cotyledon as explants.

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Author Biographies

Kanakapura K. NAMITHA, Fruit and Vegetable Technology Department, CSIR-Central Food Technological Research Institute, Mysore-570020
Fruit and Vegetable Technology Department
Pradeep S. NEGI, Fruit and Vegetable Technology Department, CSIR-Central Food Technological Research Institute, Mysore-570020

Fruit and Vegetable Technology Department

Principal Scientist

Published
2013-05-28
How to Cite
NAMITHA, K. K., & NEGI, P. S. (2013). Morphogenetic Potential of Tomato (Lycopersicon esculentum) cv. ‘Arka Ahuti’ to Plant Growth Regulators. Notulae Scientia Biologicae, 5(2), 220-225. https://doi.org/10.15835/nsb529037
Section
Research articles
CITATION
DOI: 10.15835/nsb529037