Control of Shoot Tip Necrosis in Shoot Cultures of Portulaca grandiflora Hook

Archana SRIVASTAVA; Aruna G. JOSHI

doi:10.15835/nsb519009

Control of Shoot Tip Necrosis in Shoot Cultures of Portulaca grandiflora Hook

Authors

Archana SRIVASTAVA he Maharaja Sayajirao University of Baroda, Faculty of Science, Department of Botany, Vadodara- 390002 (IN)
Aruna G. JOSHI he Maharaja Sayajirao University of Baroda, Faculty of Science, Department of Botany, Vadodara- 390002 (IN)

DOI:

https://doi.org/10.15835/nsb519009

Keywords:

Boron; calcium chloride; calcium gluconate; nodal explants

Abstract

Portulaca grandiflora, an important medicinal plant was found to show the incidence of shoot tip necrosis (STN) in shoot cultures obtained from nodal explants in Murashige and Skoog (MS) medium with 3% sucrose along with 8 μM Kinetin and 4 μM BAP. This physiological disorder is primarily due to the deficiency of calcium and boron content in the medium. Thus in the present work varying concentration of boron which was used in the form of boric acid (0.1-0.4 mM), calcium gluconate (3-30 mM) and calcium chloride (3-30 mM) were incorporated in MS medium and its effect on STN was evaluated. The observations revealed that 18 mM calcium chloride was the optimum concentration for lowering STN in shoot cultures to 40% after four weeks.

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Published

2013-02-23

How to Cite

SRIVASTAVA, A., & JOSHI, A. G. (2013). Control of Shoot Tip Necrosis in Shoot Cultures of Portulaca grandiflora Hook. Notulae Scientia Biologicae, 5(1), 45–49. https://doi.org/10.15835/nsb519009

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Issue

Vol. 5 No. 1 (2013)

Section

Research articles

CITATION

DOI: 10.15835/nsb519009

License

Papers published in Notulae Scientia Biologicae are Open-Access, distributed under the terms and conditions of the Creative Commons Attribution License.

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