Detection of Genetic Variation and Genetic Diversity in Two Indian Mudskipper Species (Boleophthalmus boddarti, B. dussumieri) using RAPD Marker

  • Vellaichamy RAMANADEVI Annamalai University, Centre of Advanced Study in Marine Biology, 608 502 Parangipettai, Tamil Nadu (IN)
  • Muthusamy THANGARAJ Annamalai University, Centre of Advanced Study in Marine Biology, 608 502 Parangipettai, Tamil Nadu (IN)
  • Anbazhagan SURESHKUMAR Annamalai University, Centre of Advanced Study in Marine Biology, 608 502 Parangipettai, Tamil Nadu (IN)
  • Jayachandran SUBBURAJ Annamalai University, Centre of Advanced Study in Marine Biology, 608 502 Parangipettai, Tamil Nadu (IN)

Abstract

Due to the environmental changes and habitat destruction the mudskipper fish population is decreasing in recent years. To predict the fish population structure, frequent manual survey and molecular methods are widely used. Molecular markers such as RAPD, microsatellite, allozyme, D-loop haplotype are frequently adopted to assess the population structure of an organism. In this study ten- arbitrary primers were screened to estimate the genetic relationships and diversity of two mudskipper species (Boleophthalmus boddarti and B. dussumieri) in Vellar estuary, Tamilnadu, India. By this RAPD marker study, the genetic diversity (H) in B. boddarti was more (0.0116 ± 0.0066) than in B. dussumieri (0.0056 ± 0.0024) in Vellar estuary (India). The genetic distance between B. boddarti and B. dussumieri was 1.7943. By observing the species specific bands and the phylogenetic analysis it is revealed that these two species clearly deviated into separate clusters emphasizing the distinct species status.

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Published
2013-05-28
How to Cite
RAMANADEVI, V., THANGARAJ, M., SURESHKUMAR, A., & SUBBURAJ, J. (2013). Detection of Genetic Variation and Genetic Diversity in Two Indian Mudskipper Species (Boleophthalmus boddarti, B. dussumieri) using RAPD Marker. Notulae Scientia Biologicae, 5(2), 139-143. https://doi.org/10.15835/nsb528992
Section
Research articles
CITATION
DOI: 10.15835/nsb528992