Spatio Temporal Expression Pattern of an Insecticidal Gene (cry2A) in Transgenic Cotton Lines

  • Allah BAKHSH University of the Punjab, National Centre of Excellence in Molecular Biology, 87, Lahore, 53700
  • Abdul Qayyum RAO Ankara University, Faculty of Agriculture, Department of Field Crops, Ankara
  • Ahmad Ali SHAHID Ankara University, Faculty of Agriculture, Department of Field Crops, Ankara
  • Tayyab HUSNAIN Ankara University, Faculty of Agriculture, Department of Field Crops, Ankara
Keywords: cry2A gene; insect resistance management; variation; efficient

Abstract

The production of transgenic plants with stable, high-level transgene expression is important for the success of crop improvement programs based on genetic engineering. The present study was conducted to evaluate genomic integration and spatio temporal expression of an insecticidal gene (cry2A) in pre-existing transgenic lines of cotton. Genomic integration of cry2A was evaluated using various molecular approaches. The expression levels of cry2A were determined at vegetative and reproductive stages of cotton at regular intervals. These lines showed a stable integration of insecticidal gene in advance lines of transgenic cotton whereas gene expression was found variable with at various growth stages as well as in different plant parts throughout the season. The leaves of transgenic cotton were found to have maximum expression of cry2A gene followed by squares, bolls, anthers and petals. The protein level in fruiting part was less as compared to other parts showing inconsistency in gene expression. It was concluded that for culturing of transgenic crops, strategies should be developed to ensure the foreign genes expression efficient, consistent and in a predictable manner.

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Published
2012-11-06
How to Cite
BAKHSH, A., RAO, A. Q., SHAHID, A. A., & HUSNAIN, T. (2012). Spatio Temporal Expression Pattern of an Insecticidal Gene (cry2A) in Transgenic Cotton Lines. Notulae Scientia Biologicae, 4(4), 115-119. https://doi.org/10.15835/nsb448217
Section
Research articles