Comparison of Cuminaldehyde Contents from Cell Suspension Cultures and Seeds of [Bunium persicum (Boiss.) B. Fedtsch.]

  • Sara KHOSRAVINIA University of Ferdowsi, Faculty of Agriculture, Mashhad
  • Seyed Mahdi ZIARATNIA Research Institute of Food Science and Technology (RIFST), P.O. Box: 91735-139, km 12 Quchan highway, Mashhad
  • Abdolreza BAGHERI University of Ferdowsi, Faculty of Agriculture, Mashhad
  • Ghadir RAJABZADEH Research Institute of Food Science and Technology (RIFST), P.O. Box: 91735-139, km 12 Quchan highway, Mashhad
  • Seyed Hassan MARASHI University of Ferdowsi, Faculty of Agriculture, Mashhad

Abstract

The cell suspension culture and seed samples of Bunium persicum were extracted by supercritical fluid, hydrodistillation and solvent methods and analyzed by Gas Chromatography. In this study to compare the different methods of extractions, cuminaldehyde was targeted as one of the Black zira essential oil constitute. For callus induction the germinated seeds were cultured as explants on Murashige and Skoog medium supplemented with 2 mg/l 2,4-dichlorophenoxy acetic acid and 0.5 mg/l kinetin (treatment A) as well as 2 mg/l ?-naphthalene acetic acid and 0.5 mg/l 6-benzyl aminopurine (treatment B) and followed by cells suspension cultures establishment for the first time. The results of cell culture showed that cells from treatment B have a growth rate higher than A. All extracts were dissolved in 1 ml hexane and analyzed by Gas Chromatography. According to the Gas Chromatography analysis, cuminaldehyde was not detected in the supercritical fluid samples, while it was present in hydrodistillation and solvent extract. Cuminaldehyde percentage in cell and seed solvent extracts was 4.65% and 18.61% respectively. Gas Chromatography results also showed that no cuminaldehyde is present in media extracts, means no cuminaldehyde has been secreted into the medium.

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Published
2012-11-06
How to Cite
KHOSRAVINIA, S., ZIARATNIA, S. M., BAGHERI, A., RAJABZADEH, G., & MARASHI, S. H. (2012). Comparison of Cuminaldehyde Contents from Cell Suspension Cultures and Seeds of [Bunium persicum (Boiss.) B. Fedtsch.]. Notulae Scientia Biologicae, 4(4), 49-54. https://doi.org/10.15835/nsb447963
Section
Review articles