Genetic Diversity Analysis of Lates calcarifer (Bloch 1790) in Captive and Wild Populations Using RAPD Markers

  • Muthusamy RAJASEKAR Annamalai University, Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Parangipettai, Tamil Nadu, 608 502 (IN)
  • Muthusamy THANGARAJ Annamalai University, Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Parangipettai, Tamil Nadu, 608 502 (IN)
  • Thathiredypalli R. BARATHKUMAR Annamalai University, Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Parangipettai, Tamil Nadu, 608 502 (IN)
  • Jayachandran SUBBURAJ Annamalai University, Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Parangipettai, Tamil Nadu, 608 502 (IN)
  • Kaliyan MUTHAZHAGAN Annamalai University, Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Parangipettai, Tamil Nadu, 608 502 (IN)

Abstract

Lates calcarifer (Bloch 1790) is one of the major economically important cultivable fish species in India. In this study, three populations of L. calcarifer was selected to assess the genetic diversity. Of which, two wild (Mudaslodai, Muthupettai) and one captive (Mutukadu) population. The genetic diversity of three populations of this species was studied using Random Amplified Polymorphic DNA (RAPD) markers. Ten random primers were used for the assessment of their genetic diversity and construction of the dendrogram. A total of 589 scorable bands were obtained, 93.12% of them were polymorphic. The Nei’s gene diversity (H) of two wild populations were more (0.0504 ± 0.0670 and 0.0519 ± 0.0953) than the captive population (0.0489 ± 0.0850). The clustering pattern obtained by UPGMA method emphasized the wild populations were clustered in one clade and captive population was deviated into another clade. This study proved that RAPD analysis has the ability to discriminate L. calcarifer populations. Further molecular studies, comprising a higher number of molecular tools are still required to precisely evaluate the genetic structure of all seabass populations along the Indian coast.

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Published
2012-08-30
How to Cite
RAJASEKAR, M., THANGARAJ, M., BARATHKUMAR, T. R., SUBBURAJ, J., & MUTHAZHAGAN, K. (2012). Genetic Diversity Analysis of Lates calcarifer (Bloch 1790) in Captive and Wild Populations Using RAPD Markers. Notulae Scientia Biologicae, 4(3), 33-37. https://doi.org/10.15835/nsb437896
Section
Research articles
CITATION
DOI: 10.15835/nsb437896