In Vitro Selection of Foeniculum vulgare for Salt Tolerance


  • Azita KHORAMI Islamic Azad University of Mashhad (IR)
  • Abbas SAFARNEJAD Razavi Khorasan Agricultural and Natural Resources Research Center, P.O.Box 1148/91735, Mashhad (IR)



In vitro selection of Foeniculum vulgare for salt tolerance was undertaken by the use of somaclonal variation. In this idea, explants of root, hypocotyl and cotyledon of sterilized seedling were transferred to callus and regeneration media with concentrations of 0, 50, 100 and 150 mM of NaCl. After 4 weeks, calli induction, regeneration frequency and calli fresh and dry weights, in both control and stress conditions, were measured. The results showed that salinity caused a significant decrease in the callus induction and shoot regeneration of fennel. However, in the presence of 100 and 150 mM NaCl, the highest frequency of callus induction in hypocotyl and cotyledon explants was recorded on the media supplemented with 1 mg l-1 IAA (Indol-3-Acetic Acid) plus 1 mg l-1 2,4-D (2, 4- dichlorophenoxyacetic acid) and 2 mg l-1 kinetin. Among different growth regulator treatments, the combination of 2 mg l-1 NAA (Naphtaleneacetic acid) and 0.5 mg l-1 kinetin was found to be the most effective for shoot regeneration under stress condition. The highest dose of NaCl (150 mM) inhibited callus induction and shoot regeneration compared to control with 41% and 96% respectively. The calli fresh and dry weights of all explants were decreased with the increas of NaCl concentration. The highest and the lowest of dry and fresh weight of calli were observed in 0 and 150 mM respectively.


Metrics Loading ...

Author Biography

Azita KHORAMI, Islamic Azad University of Mashhad

Dept. of Natural Resources, Associate Prof.




How to Cite

KHORAMI, A., & SAFARNEJAD, A. (2011). In Vitro Selection of Foeniculum vulgare for Salt Tolerance. Notulae Scientia Biologicae, 3(2), 90–97.



Research articles
DOI: 10.15835/nsb325806