Short-term changes in antioxidant response of Eutrema salsugineum exposed to severe salinity
Keywords:Eutrema salsugineum, halophytes, hydrogen peroxide, salt stress, signal molecule, oxidative stress
Salinity is one of the major constraints limiting plant productivity. Understanding the mechanism of salinity tolerance is a necessary step for improving crops yield in saline conditions. Eutrema salsugineum is a halophyte species and plant model for salinity tolerance mechanism study. Therefore, we studied the ability of E. salsugineum plants for their antioxidant response to 400 mM NaCl treatment. Changes in hydrogen peroxide (H2O2), malondialdehyde (MDA) and tocopherol contents, and kinetics of catalase, superoxide dismutase and peroxidase enzyme activities were investigated. Results shows that, although increasing in the beginning of the salt treatment, the fast decrease in H2O2 and MDA levels afterwhile indicates the ability of E. salsugineum to alleviate oxidative damage and to maintain membrane integrity. Together, with the increase of tocopherol contents, the high enzymatic activity of CAT, SOD and POD reflect the highly efficient antioxidant response of E. salsugineum.
Bradford MM (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72(1-2):248-254. https://doi.org/10.1006/abio.1976.9999
Cela J, Chang C, Munné-Bosch S (2011). Accumulation of γ-rather than α-tocopherol alters ethylene signaling gene expression in the vte4 mutant of Arabidopsis thaliana. Plant and Cell Physiology 52(8):1389-1400. https://doi.org/10.1093/pcp/pcr085
Chaves MM, Flexas J, Pinheiro C (2009). Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell. Annals of Botany 103(4):551-560. https://doi.org/10.1093/aob/mcn125
Ellouzi H, Ben Hamed K, Cela J, Munné-Bosch S, Abdelly C (2011). Early effects of salt stress on the physiological and oxidative status of Cakile maritima (halophyte) and Arabidopsis thaliana (glycophyte). Physiologia Plantarum 142:128-143. https://doi.org/10.1111/j.1399-3054.2011.01450.x
Ellouzi H, Hamed KB, Hernández I, Cela J, Müller M, Magné C, Munné-Bosch S (2014). A comparative study of the early osmotic, ionic, redox and hormonal signaling response in leaves and roots of two halophytes and a glycophyte to salinity. Planta 240:1299-1317. https://doi.org/10.1007/s00425-014-2154-7
Ghars MA, Parre E, Debez A, Bordenave M, Richard L, Leport L, Bouchereau A, Savoure A, Abdelly C (2008). Comparative salt tolerance analysis between Arabidopsis thaliana and Thellungiella halophila, with special emphasis on K+/Na+ selectivity and proline accumulation. Journal of Plant Physiology 165:588-599. https://doi.org/10.1016/j.jplph.2007.05.014
Gossett DR, Millhollon EP, Lucas MC (1994). Antioxidant response to NaCl stress in salt-tolerant and salt-sensitive cultivars of cotton. Crop Sciences 34:706-714. https://doi.org/10.2135/cropsci1994.0011183X003400030020x
Ivushkin K, Bartholomeus H, Bregt AK, Pulatov A, Kempen B, De Sousa L (2019). Global mapping of soil salinity change. Remote Sensing of Environment 231:111260. https://doi.org/10.1016/j.rse.2019.111260
Liu ZL, He XY, Chen W, Yuan FH, Yan K, Tao DL (2009). Accumulation and tolerance characteristics of cadmium in a potential hyperaccumulator Lonicera japonica Thunb. Journal of Hazardous Materials 169:170-175. https://doi.org/10.1016/j.jhazmat.2009.03.090
Lugan R, Niogret MF, Leport L, Guegan JP, Larher FR, Savouré A, Kopka J, Bouchereau A (2010). Metabolome and water homeostasis analysis of Thellungiella salsuginea suggests that dehydration tolerance is a key response to osmotic stress in this halophyte. Plant Journal 64:215-229. https://doi.org/10.1111/j.1365-313x.2010.04323.x
Miller G, Suzuki N, Ciftci-Yilmaz S, Mittler R (2010). Reactive oxygen species homeostasis and signalling during drought and salinity stresses. Plant Cell and Environment 33:453-467. https://doi.org/10.1111/j.1365-3040.2009.02041.x
Mittler R, Vanderauwera S, Suzuki N (2011). ROS signaling: the new wave? Trends in Plant Sciences 16:300-309. https://doi.org/10.1016/j.tplants.2011.03.007
Mullineaux P, Karpinski S (2002). Signal transduction in response to excess light: getting out of the chloroplast. Current Opinion in Plant Biology 5:43-48. https://doi.org/10.1016/s1369-5266(01)00226-6
Munns R, Tester M (2008). Mechanisms of salinity tolerance. Annual Revue in Plant Biology 59:651-681. https://doi.org/10.1146/annurev.arplant.59.032607.092911
Munns R (2002). Comparative physiology of salt and water stress. Plant Cell and Environment 25:239-250. https://doi.org/10.1046/j.0016-8025.2001.00808.x
Peng X, Wang N, Sun S, Geng L, Guo N, Liu A, Ahammed GJ (2023). Reactive oxygen species signaling is involved in melatonin-induced reduction of chlorothalonil residue in tomato leaves. Journal of Hazardous Materials 443:130212. https://doi.org/10.1016/j.jhazmat.2022.130212
Scalet M, Federico R, Guido MC, Manes F (1995). Peroxidase activity and polyamine changes in response to ozone and simulated acid rain in Aleppo pine needles. Environmental and Experimental Botany 35:417-425. https://doi.org/10.1016/0098-8472(95)00001-3
Scebba F, Sebastiani L, Viyagliano C (1999). Protective enzymes against activated oxygen species in wheat (Triticum aestivum L.) seedling: response to cold acclimation. Journal of Plant Physiology 155:762-768. https://doi.org/10.1016/S0176-1617%2899%2980094-7
Singh D (2022). Juggling with reactive oxygen species and antioxidant defense system–a coping mechanism under salt stress. Plant Stress 5:100093. https://doi.org/10.1016/j.stress.2022.100093
Stevens PW, Fox SL, Montague CL (2009). The interplay between mangroves and saltmarshes at the transition between temperate and subtropical climate in Florida. Wetlands Ecology and Management 14:435-444. http://dx.doi.org/10.1007/s11273-006-0006-3
Volkov V, Wang B, Dominy PJ, Fricke W, Amtmann A (2003). Thellungiella halophila, a salt-tolerant relative of Arabidopsis thaliana, possesses effective mechanisms to discriminate between potassium and sodium. Plant Cell and Environment 27:1-14. https://doi.org/10.1046/j.0016-8025.2003.01116.x
Wojtyla Ł, Lechowska K, Kubala S, Garnczarska M (2016). Different modes of hydrogen peroxide action during seed germination. Frontiers in Plant Science 7:66. https://doi.org/10.3389/fpls.2016.00066
Zhou Y, Chen S, Hu B, Ji W, Li S, Hong Y, Shi Z (2022). Global soil salinity prediction by open soil Vis-NIR spectral library. Remote Sensing 14(21):5627. https://doi.org/10.3390/rs14215627
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