Anatomical and morphological structure of the leaf of the genus Malus spp.


  • Inna GONCHAROVSKA National Academy of Sciences of Ukraine, M.M. Gryshko National Botanical Garden, Tymiryazevska str. 1, 01014 Kyiv (UA)
  • Iwona SZOT University of Life Sciences, Faculty of Horticulture and Landscape Architecture, Lublin (PL)



epidermis structure, Malus, trichomes, stoma


The article describes changes in the structure, leaves, (including mesophyll structure, structure of the midrib, distribution of stomata, and the number of vascular bundles) in Malus during the growing season (from June to August) under the influence of prolonged drought. The leaves anatomy and morphology were investigated by methods of light and scanning electron microscopy. The study was carried out on four representatives of the genus Malus: M. niedzwetzkyana Borkh., M. baccata ‘Pendula’, M. domestica ‘Eliza Rathke’, M. domestica ‘Renet Simirenko’. The influence of drought on the size of the mesophyll of the leaf plate and the number of stomata along the stretch from June to August was studied. The cell circumference of the sclerenchyma ranged from μm in 27.33 (M. niedzwetzkyana) to 81.92 μm (in M. domestica ‘Elise Rathke’). The number of stomata varied from 214.0 units in (M. domestica ‘Renet Simirenko’) to 304.0 units in (M. baccata ‘Pendula’), length from 11.6 μm (M. baccata ‘Pendula’) to 28.63 μm (M. domestica ‘Elise Rathke’), width from 9.4 μm (M. domestica ‘Elise Rathke’) up to 21.8 μm (M. domestica ‘Elise Rathke’) μm. The length of the trichomes varied from 409.92 μm (M. domestica ‘Renet Simirenko’) to 745.09 μm (M. niedzwetzkyana). Also, the anocytic stomatal type, the number, different stomata and trichomes, the presence of wax on the cuticle, and the found druses of calcium oxalate in M. baccata ‘Pendula’, can be important for systematic phylogeny. Thanks to these studies, it is possible to suggest physiological adaptability of apple trees to prolonged drought, as well as to identify more resistant phenotypes for the breeding process to develop resistant cultivars.


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Azevedo AM, Andrade Junior VC, Oliveira CM, Fernandes JSC, Pedrosa CE, Dornas MFS, Castro BMC (2013). Selection of lettuce genotypes for protected cultivation: Genetic divergence and importance of characters. Horticultura Brasileira 31:260-265.

Bacelar E, Correia C, Moutinho-Pereira J, Goncxalves B, Lopes J, Torres-Pereira J (2004). Sclerophylly and leaf anatomical traits of five field-grown olive cultivars growing under drought conditions. Tree Physiology 24:233-239.

Balkwill MJ, Balkwill K (2002). A preliminary analysis of distribution patterns in a large, pantropical genus, Barleria L. (Acanthaceae). Journal of Biogeography 25:95-110.

Barthlott W, Neinhuis C, Cutler D., Ditsch F, Meusel I, Theisen I, Wilhelmi H (1998). Classification and terminology of plant epicuticular waxes. Botanical Journal of the Linnean Society 126:237-260.

Beer C (2010). Terrestrial gross carbon dioxide uptake: Global distribution and covariation with climate. Science 329:834-838.

Campbell CS, Evans RC, Morgan DR, Dickinson TA, Arsenault MP (2007). Phylogeny of subtribe Pyrinae (formerly the Maloideae, Rosaceae): limited resolution of complex evolutionary history. Plant Systematics and Evolution 266:119-145.

Castanheira DT, Rezende TT, Baliza DP, Guedes JM, Carvalho SP, Guimaraes RJ, Viana MTR (2016). Potencial de utilizac xao de características anatomicas e fisiol ogicas na selec xao de progenies de cafeeiro. Coffee Science 11:374-385.

Forte AV, Ignatov Ponomarenko AN, Dorokhov VV, Savelyev NI (2002). Phylogeny of the Malus (apple tree) species, inferred from the morphological traits and molecular DNA analysis. Russian Journal of Genetics 38(10):1150-1160.

Franceschi V (2001). Calcium oxalate in plants. Trends in Plant Science 6:331.

Gangaram S, Naidoo Y, Dewir YH (2020). Foliar micromorphology, ultrastructure, and histochemical analysis of Barleria albostellata C.B. Clarke. South African Journal of Botany 135:212-224.

Golubkova IM (2015). Features of the anatomical structure of the leaf species and varieties of Persica Mill. In the right-wing Forest-Steppe of Ukraine 'Young Scientist' 2(17):30-32.

Goncharovska IV, Kuznetsov VV, Galushko VM, Antonyuk GO (2017). Drought resistance of apple hybrids with the participation of the cultivar Vydubytska plakucha in the conditions of the Forest-Steppe of Ukraine. Introduction of Plants 3:88-94.

Goncharovska I, Levon V (2021). Content of anthocyanins in the bark of fruit and berry plants due to adaption to low temperatures. Khimiya Rastitel'nogo Syr'ya 1:233-239.

Grisi FA, Alves JDA, Castro EC, Oliveira C, Biagiottis G, Melo LA (2008). Leaf anatomical evaluations in 'Catuaí' and 'Siriema' coffee seedlings submitted to water stress. Ciência e Agrotecnologia 32:1730-1736.

Harbage JF, Stimart DP, Evert RF (2019). Anatomy of adventitious root formation in microcuttings of Malus domestica Borkh. `Gala'. Journal of the American Society for Horticultural Science 118(5):680-688

Humphrey V (2018). Sensitivity of atmospheric CO2 growth rate to observed changes in terrestrial water storage. Nature 560:628-631.

Jackson JE, Beakbane AB (1970). Structure of leaves growing at different light intensities within mature apple trees. Annual Report East Malling Research Station 1969:87-89.

Klimenko SV (2016). Anatomy of leaves of members of the family Cornaceae J. Presl in the forest-steppe conditions of Ukraine Introduction of plants. Kiev 3:23-37.

Medri C, Medri ME, Ruas EA., Souza LA, Medri PS, Sayhun S, Bianchini E, Pimenta JA (2011). Morpho-anatomy of vegetative organs in seedlings of Aegiphila sellowiana Cham. (Lamiaceae) subject to flooding. Acta Botanica Brasilica 25:445-454.

Metcalfe CR, Chalk L (1965). Anatomy of Dicotyledons. Clarendon Press, Oxford, pp 539-550.

Mojena R (1977). Hierarchical grouping methods and stopping rules: An evaluation. The Computer Journal 20:359-363.

Phipps JB, Robertson KR, Smith PG, Rohrer JR (1990). A checklist of the subfamily Maloideae (Rosaceae). Canadian Journal of Botany 68:2209-2269.

Potter D, Eriksson T, Evans RC, Oh S, Smedmark JEE, Morgan DR, … Campbell CS (2007). Phylogeny and classification of Rosaceae. Plant Systematics and Evolution 266:5-43.

Pyykkö M (1966). The leaf anatomy of East Patagonian xeromorphic plants. In: Annales Botanici Fennici, pp 453-622.

Robinson JP, Harris SA, Juniper BE (2001). Taxonomy of the genus Malus MIill. (Rosaceae) with emphasis on the cultivated apple, Malus domestica Borkh. Plant Systematics and Evolution 226:35-58.

Sokolova A (2013). Microscopical diagnostics of Tilia L. genus species of amur region according to leaf structure Альманах современной науки и образования 11(78):164-167.

Stace C (1965). Cuticular studies as an aid to plant taxonomy. The Bulletin of the British Museum (National History) 4:37-40.

Vinogradova Y, Riabchenko A, Gorbunov Yu, Grygorieva O, Brindza J (2018). Characteristic of stomata for Cydonia oblonga Mill., Pseudocydonia sinensis (Thouin) C.K. Schneid. and Chaenomeles japonica (Thunb.) Lindl. ex Spach species Annals of the Romanian Society for Cell Biology 22(2):18-25.

Vlam M, Baker PJ, Bunyavejchewin S, Zuidema PA (2014). Temperature and rainfall strongly drive temporal growth variation in Asian tropical forest trees. Oecologia 174:1449-1461.

Xie R, Zhao J, Lu L, Brown P, Guo J, Tian S (2020). Penetration of foliar-applied Zn and its impact on apple plant nutrition status: in vivo evaluation by synchrotron-based X-ray fluorescence microscopy. Horticulture Research 7:147.

Zieliński J, Guzicka M, Tomaszewski D, Maciejewska-Rutkowska I. (2010). Pericarp anatomy of wild roses (Rosa L., Rosaceae). Flora – Morphology, Distribution, Functional Ecology of Plants 205(6):363-369



How to Cite

GONCHAROVSKA, I., & SZOT, I. (2023). Anatomical and morphological structure of the leaf of the genus Malus spp. Notulae Scientia Biologicae, 15(1), 11449.



Research articles
DOI: 10.55779/nsb15111449

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