Antagonism and plant growth promoting traits of actinomycetes isolated from the rhizosphere of halophyte Atriplex halimus L.

Inas BOUKELLOUL; Lamia AOUAR; Mohamed CHEKARA BOUZIANI; Amar ZELLAGUI; Mouna DERDOUR; Youcef NECIB

doi:10.55779/nsb15111437

Authors

Inas BOUKELLOUL University of Oum El Bouaghi, Department of Natural Sciences and Life, PO Box 358, Oum El Bouaghi 04000; University of Oum El Bouaghi, Laboratory of Biomolecules and Plant Breeding, Department of Natural Sciences and Life, PO Box 358, Oum El Bouaghi 04000 (DZ)
Lamia AOUAR University of Oum El Bouaghi, Laboratory of Biomolecules and Plant Breeding, Department of Natural Sciences and Life, PO Box 358, Oum El Bouaghi 04000 (DZ)
Mohamed CHEKARA BOUZIANI University of Oum El Bouaghi, Department of Natural Sciences and Life, PO Box 358, Oum El Bouaghi 04000; University Mentouri Constantine 1, Laboratory of Genetic Biochemistry and Plant Biotechnology, Constantine (DZ)
Amar ZELLAGUI University of Oum El Bouaghi, Department of Natural Sciences and Life, PO Box 358, Oum El Bouaghi 04000; University of Oum El Bouaghi, Laboratory of Biomolecules and Plant Breeding, Department of Natural Sciences and Life, PO Box 358, Oum El Bouaghi 04000 (DZ) https://orcid.org/0000-0002-6515-8103
Mouna DERDOUR National Center for Biotechnology Research, UV 03 PO Box E73 Constantine 25000 (DZ)
Youcef NECIB University Mentouri Constantine 1, Laboratory of Microbiological Engineering and Applications, Constantine (DZ)

DOI:

https://doi.org/10.55779/nsb15111437

Keywords:

actinomycetes, antagonism, Atriplex halimus, PGPR, rhizospheric soil

Abstract

Biocontrol is considered as an effective alternative to the application of agrochemicals, which are harmful to the environment, human, and animal health. In this study, twenty-six strains of actinomycetes were isolated from rhizospheric arid soil of the halophyte Atriplex halimus L. ῾Guettaf’ in Biskra province, Algeria. The six isolates that have inhibited at least three phytopathogenic fungi among the five tested (Fusarium oxysporum, Alternaria alternata, Fusarium solani, Aspergillus flavus and Botrytis cinerea) were selected, and have been tested in vitro against phytopathogenic bacteria (Pectobacterium carotovorum and Streptomyces scabies). They were also evaluated for their ability to hydrolyze phosphate, elaborate siderophores, produce indole-3-acetic acid (IAA), and to antagonize S. scabies in vivo (on radish seedlings). Based on the physicochemical analyses, soil samples were categorized as alkaline and extremely-saline. The antagonism results revealed varying antifungal potential among the selected isolates (Act11, Act16, Act17, Act18, Act23 and Act24), about 50% were able to inhibit the growth of F. solani and A. flavus, followed by 33.33% of those having antagonized F. oxysporum, while A. alternata was found to be the most sensitive. Only Act18 has antagonized S. scabies in vitro with an inhibition diameter zone of 19 ± 0.41 mm. However, in vivo trials showed that four isolates have counteracted S. scabies. Among them, Act18 and Act24 have significantly and positively affected the root surface (P = 0.0062) and prevented common scab. IAA was detected in all selected isolates with Act24 being the highest producer (77.45 μg mL⁻¹). Additionally, degradation ability revealed that four isolates were able to hydrolyze phosphate while three exhibited the capacity of elaborating siderophores. The six isolates were assigned to Streptomyces genius according to their morphological, physiological and chemotaxonomical traits. Based on this study, Streptomyces sp. Act18 and Streptomyces sp. Act24 that tolerate 7.5% NaCl concentration, prevent common scab and exhibit some plant growth attributes, may be considered as promising biocontrol agents to be applied in arid and saline soils.

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