Morphological and cultural characterization of two Fusarium isolates causing wheat fusarium head blight in Algeria

Salah  HADJOUT; Mohamed  ZOUIDI

doi:10.55779/nsb14411318

Authors

Salah HADJOUT Territory Planning Research Center, CRAT, Zouaghi Slimane 25000, Constantine; Higher National School of Agronomy, Laboratory of Phytopathology and Molecular Biology, ENSA, Algiers (DZ)
Mohamed ZOUIDI Territory Planning Research Center, CRAT, Zouaghi Slimane 25000, Constantine (DZ)

DOI:

https://doi.org/10.55779/nsb14411318

Keywords:

Algeria, fusarium head blight, Fusarium isolates, morphological and cultural characterization

Abstract

In Algeria, fusarium head blight is a serious fungal disease of wheat caused by Fusarium genus. The study of the epidemiological cycle of the disease in the field is always hampered by a major problem which is the identification of the parasitic complex of Fusarium species. These species can only be identified after Petri dish culture and observation of a set of morphological criteria such as color, cultural aspect of colonies, growth rate of mycelium and the form of isolate macroconidia. To this end, the objective of this present study is to identify two Fusarium isolates based on a set of morphological and cultural criteria mentioned above. The results obtained for all the morphological characters studied show phenotypic variability between the two isolates. Based on these results, we were able to characterize these two isolates as F. graminearum and F. culmorum. Indeed, morphological and cultural characterization, although important, remains insufficient. It should therefore be further investigated by molecular characterization, in order to highlight any differences between the two isolates studied.

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References

Abdallah-Nekache N, Laraba I, Ducos C, Barreau C, Bouznad Z, Boureghda H (2019). Occurrence of fusarium head blight and fusarium crown rot in Algerian wheat: identification of associated species and assessment of aggressiveness. European Journal of Plant Pathology 154(3):499-512. https://doi.org/10.1007/s10658-019-01673-7

Akshay Kumar HM, Saharan MS, Aggarwal R, Gurjar MS, Nallathambi P (2021). In vitro mycelium growth variation among Fusarium graminearum isolates causing head blight of wheat in India. Journal of Pharmacognosy and Phytochemistry 10(1):1417-1419.

Alves-Santos FM, Benito EP, Eslava AP, Díaz-Mínguez J (1999). Genetic diversity of Fusarium oxysporum strains from common bean fields in Spain. Applied and Environmental Microbiology 65(8):3335-3340. https://doi.org/10.1128/AEM.65.8.3335-3340.1999

Aoki T, O'Donnell K (1999). Morphological and molecular characterization of Fusarium pseudograminearum sp. nov., formerly recognized as the Group 1 population of F. graminearum. Mycologia 91(4):597-609. https://doi.org/10.1080/00275514.1999.12061058

Beacorn JA, Thiessen LD (2021). First report of Fusarium lacertarum causing fusarium head blight on sorghum in North Carolina. Plant Disease 105(3):699-699. https://doi.org/10.1094/PDIS-05-20-1012-PDN

Booth C (1985). The genus Fusarium. Ed. Common Weath Mycological Institute. pp 237.

Brennan JM, Fagan B, Van Maanen A, Cooke BM, Doohan FM (2003). Studies on in vitro growth and pathogenicity of European Fusarium fungi. European Journal of Plant Pathology 109(6):577-587.

Campbell KAG, Lipps PE (1998). Allocation of resources: sources of variation in fusarium head blight screening nurseries. Phytopathology 88(10):1078-1086. https://doi.org/10.1094/PHYTO.1998.88.10.1078

Cerón-Bustamante M, Ward TJ, Kelly A, Vaughan MM, McCormick SP, Cowger C, … Nava-Díaz C (2018). Regional differences in the composition of fusarium head blight pathogens and mycotoxins associated with wheat in Mexico. International Journal of Food Microbiology 273:11-19. https://doi.org/10.1016/j.ijfoodmicro.2018.03.003

Davari M, Safaie N, Darvishnia M, Didar Taleshmikaeel R (2014). Occurrence of deoxynivalenol producing isolates of Fusarium graminearum species complex associated with head blight of wheat in Moghan area. Journal of Crop Protection 3(2):113-123.

Divakara ST, Santosh P, Aiyaz M, Venkata Ramana M, Hariprasad P, Nayaka SC, Niranjana SR (2014). Molecular identification and characterization of Fusarium spp. associated with sorghum seeds. Journal of the Science of Food and Agriculture 94(6):1132-1139. https://doi.org/10.1002/jsfa.6380

Figueroa M, Hammond‐Kosack KE, Solomon PS (2018). A review of wheat diseases - a field perspective. Molecular Plant Pathology 19(6):1523-1536. https://doi.org/10.1111/mpp.12618

Gallé Á, Pelsőczi A, Benyó D, Podmaniczki A, Szabó-Hevér Á, Poór P, Tóth B, … Csiszár J (2022). Systemic response to Fusarium graminearum and culmorum inoculations: changes in detoxification of flag leaves in wheat. Cereal Research Communications 1-9. https://doi.org/10.1007/s42976-022-00272-3

Ghimire B, Martinez-Espinoza AD, Ghimire B, Harrelson BC, Youmans J, Mergoum M, Buck JW (2021). First report of Fusarium poae causing Fusarium head blight of wheat in Georgia, USA. Plant Disease 105(2):491-491. https://doi.org/10.1094/PDIS-08-20-1779-PDN

Gómez D, Salvador P, Sanz J, Casanova J L (2021). Modelling wheat yield with antecedent information, satellite and climate data using machine learning methods in Mexico. Agricultural and Forest Meteorology 300:108317. https://doi.org/10.1016/j.agrformet.2020.108317

Hadjout S, Chéreau S, Atanasova-Penichon V, Marchegay G, Mekliche L, Boureghda H, … Richard-Forget F (2017). Phenotypic and biochemical characterization of new advanced Durum wheat breeding lines from Algeria that show resistance to fusarium head blight and to mycotoxin accumulation. Journal of Plant Pathology 99(3):671-680.

Hadjout S, Chéreau S, Mekliche L, Marchegay G, Ducos C, Boureghda H, … Richard-Forget F (2022). Molecular identification of some Fusarium isolates and their chemotypes involved in fusarium head blight on Durum wheat in Algeria, Archives of Phytopathology and Plant Protection 55(4):499-513. https://doi.org/10.1080/03235408.2022.2034363

Haque MA, Wang Y, Shen Z, Li X, Saleemi MK, He C (2020). Mycotoxin contamination and control strategy in human, domestic animal and poultry: A review. Microbial Pathogenesis 142:104095. https://doi.org/10.1016/j.micpath.2020.104095

Houmairi H, Oubayoucef A, Idrissi I, Krimi FB (2018). Haute prévalence de Fusarium spp. associés aux grains de céréales dans la région centrale du Maroc: risques pathogénique et toxinogène. Revue Marocaine des Sciences Agronomiques et Vétérinaires 6(3):355-361.

Jaillais B, Roumet P, Pinson-Gadais L, Bertrand D (2015). Detection of fusarium head blight contamination in wheat kernels by multivariate imaging. Food Control 54:250-258. https://doi.org/10.1016/j.foodcont.2015.01.048

Kebede M, Adugna G, Hundie B (2020). Identification of Fusarium species responsible to cause wheat head blight in Southwestern Ethiopia. Research Journal of Plant Pathology 3(1):3. https://doi.org/10.36648/plantpathology.3.1.03

Lecellier A (2013). Caractérisation et identification des champignons filamenteux par spectroscopie vibrationnelle. Reims: Université de Reims Champgne-Ardenne 195:9-27.

Leyva-Mir SG, García León E, Camacho-Tapia M, Villasenor-Mir HE, Leyva-Madrigal KY, Mora-Romero GA, Tovar-Pedraza JM (2022). Occurrence of the Fusarium incarnatum-equiseti species complex causing fusarium head blight of wheat in Mexico. Plant Disease. https://doi.org/10.1094/PDIS-11-21-2467-PDN

Maresca M (2013). From the gut to the brain: Journey and pathophysiological effects of the food-associated trichothecene mycotoxin deoxynivalenol. Toxins 5(4):784-820. https://doi.org/10.3390/toxins5040784

McMullen M, Bergstrom G, De Wolf E, Dill-Macky R, Hershman D, Shaner G, Van Sanford D (2012). A unified effort to fight an enemy of wheat and barley: Fusarium head blight. Plant Disease 96(12):1712-1728. https://doi.org/10.1094/PDIS-03-12-0291-FE

Mohammed-Ameen MK, Minati MH, Abbas M (2021). Morphogenetic identification, description and pathogenicity of novel pathogens on Iraqi wheat plant (Triticum aestivum) causing head blight and crown rot diseases. Biodiversitas Journal of Biological Diversity 22(5):2999-3005. https://doi.org/10.13057/biodiv/d220565

Nelson PE, Toussoun TA, Marasas WFO (1983). Fusarium species: an illustrated manual for identification. Pennsylvania State University Press, University Park, Pennsylvania.

Shewry PR, Hey SJ (2015). The contribution of wheat to human diet and health. Food and Energy Security 4(3):178-202. https://doi.org/10.1002/fes3.64

Stępień Ł, Chełkowski J (2010). Fusarium head blight of wheat: pathogenic species and their mycotoxins. World Mycotoxin Journal 3(2):107-119. https://doi.org/10.3920/WMJ2009.1193

Ortoneda M, Capilla J, Pastor FJ, Pujol I, Guarro J (2004). In vitro interactions of licensed and novel antifungal drugs against Fusarium spp. Diagnostic Microbiology and Infectious Disease 48(1):69-71. https://doi.org/10.1016/j.diagmicrobio.2003.09.003

Pancaldi D, Tonti S, Prodi A, Salomoni D, Dal Pra M, Nipoti P, Alberti I, Pisi A (2010). Survey of the main causal agents of fusarium head blight of durum wheat around Bologna, northern Italy. Phytopathologia Mediterranea 49(2):258-266.

Parry DW, Jenkinson P, McLeod L (1995) Fusarium ear blight (scab) in small grain cereals - a review. Plant Pathology 44(2):207-238. https://doi.org/10.1111/j.1365-3059.1995.tb02773.x

Polišenská I, Vaculová K, Jirsa O, Sedláčková I, Frydrych J (2019). Yield and quality of two hulless barley varieties after inoculation with Fusarium culmorum. Kvasny Prumysl 65(1):17-22. https://doi.org/10.18832/kp2019.65.17

Prat N, Guilbert C, Prah U, Wachter E, Steiner B, Langin T, Robert O, Buerstmayr H (2017). QTL mapping of fusarium head blight resistance in three related durum wheat populations. Theoretical and Applied Genetics 130(1):13-27. https://doi.org/10.1007/s00122-016-2785-0

Quero L (2018). Développement de la spectrométrie de masse MALDI-TOF pour l'identification des champignons filamenteux d'intérêt alimentaire et étude de leur résistance aux molécules biocides (Doctoral dissertation, Brest).

Rapilly F (1968). Les techniques de mycologie en pathologie végétale. Annales des Epiphyties. 19. Hors-Série. Pp 102.

Rebouh NY, Polityko PM, Pakina E, Plushikov VG, Norezzine A, Gadzhikurbanov A, … Iguer-Ouada M (2019). Impact of three integrated crop protection treatments on the varieties of winter wheat (Triticum aestivum L.) in Moscow area, Russia. Research on Crops 20(1):161-168. https://doi.org/10.31830/2348-7542.2019.022

Ristaino JB, Anderson PK, Bebber DP, Brauman KA, Cunniffe NJ, Fedoroff NV, ... Wei Q (2021). The persistent threat of emerging plant disease pandemics to global food security. Proceedings of the National Academy of Sciences 118(23):e2022239118. https://doi.org/10.1073/pnas.2022239118

Rizzo DM, Lichtveld M, Mazet JA, Togami E, Miller SA (2021). Plant health and its effects on food safety and security in a One Health framework: Four case studies. One Health Outlook 3(1):1-9. https://doi.org/10.1186/s42522-021-00038-7

Senatore MT, Ward TJ, Cappelletti E, Beccari G, McCormick SP, Busman M, Laraba I, O’Donnell K, Prodi A (2021). Species diversity and mycotoxin production by members of the Fusarium triticum species complex associated with fusarium head blight of wheat and barley in Italy. International Journal of Food Microbiology 358:109298. https://doi.org/10.1016/j.ijfoodmicro.2021.109298

Toussoun TA, Nelson PE (1976). Fusarium: A pictorial guide to the identification of Fusarium species according to the taxonomy system of Snyder and Hansen. Second edition, the Pennsylvania State University Press, pp 43.

Wagacha JM, Muthomi JW (2007). Fusarium culmorum: Infection process, mechanisms of mycotoxin production and their role in pathogenesis in wheat. Crop Protection 26(7):877-885. https://doi.org/10.1016/j.cropro.2006.09.003

Wegulo SN (2021). Advances in understanding the epidemiology of Fusarium in cereals. In: Achieving Durable Disease Resistance in Cereals. Burleigh Dodds Science Publishing, pp. 83-109.