GA3 mediated enhanced transcriptional rate and mRNA stability of 3-hydroxy-3-methylglutaryl coenzyme a reductase 1 (NtHMGR1) in Nicotiana tabacum L.

Authors

  • Raman MANOHARLAL ITC Limited, ITC Life Science and Technology Centre (LSTC), Peenya Industrial Area, 1st Phase, Bengaluru-560058, Karnataka (IN)
  • Lucky DUHAN Maharshi Dayanand University, Department of Biochemistry, Rohtak-124001 (IN)
  • Ritu PASRIJA Maharshi Dayanand University, Department of Biochemistry, Rohtak-124001 (IN)
  • Saiprasad V.S. GANDRA ITC Limited, ITC Life Science and Technology Centre (LSTC), Peenya Industrial Area, 1st Phase, Bengaluru-560058, Karnataka (IN)

DOI:

https://doi.org/10.55779/nsb14411317

Keywords:

GA3, HMGR1, Nicotiana tabacum, transcription run-on, transcriptional rate and mRNA stability

Abstract

Our present study evaluated the underlying molecular-mechanism(s) associated with the observed enhanced transcript levels and concomitant functional activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase 1 (NtHMGR1), a rate-limiting enzyme of cytosolic mevalonate (MVA) pathway of terpenoids biosynthesis, by gibberellin A3 (GA3) treatment in model cultivated tobacco, Nicotiana tabacum L. Based on the transcription run-on and cordycepin chase assays, our results demonstrated that tobacco seeds-priming with GA3 causes a relative and significantly enhanced transcriptional rate and mRNA stability of NtHMGR1. Taken together, our study established that GA3 mediated transcriptional and post-transcriptional regulatory control as one of the mechanisms for the observed enhanced transcript-levels and consequently enhanced functional activity of NtHMGR1.

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References

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Published

2022-11-28

How to Cite

MANOHARLAL, R., DUHAN, L., PASRIJA, R., & GANDRA, S. V. . (2022). GA3 mediated enhanced transcriptional rate and mRNA stability of 3-hydroxy-3-methylglutaryl coenzyme a reductase 1 (NtHMGR1) in Nicotiana tabacum L. Notulae Scientia Biologicae, 14(4), 11317. https://doi.org/10.55779/nsb14411317

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DOI: 10.55779/nsb14411317