GA3 mediated enhanced transcriptional rate and mRNA stability of 3-hydroxy-3-methylglutaryl coenzyme a reductase 1 (NtHMGR1) in Nicotiana tabacum L.

Raman MANOHARLAL; Lucky DUHAN; Ritu PASRIJA; Saiprasad V.S.  GANDRA

doi:10.55779/nsb14411317

Authors

Raman MANOHARLAL ITC Limited, ITC Life Science and Technology Centre (LSTC), Peenya Industrial Area, 1st Phase, Bengaluru-560058, Karnataka (IN)
Lucky DUHAN Maharshi Dayanand University, Department of Biochemistry, Rohtak-124001 (IN)
Ritu PASRIJA Maharshi Dayanand University, Department of Biochemistry, Rohtak-124001 (IN)
Saiprasad V.S. GANDRA ITC Limited, ITC Life Science and Technology Centre (LSTC), Peenya Industrial Area, 1st Phase, Bengaluru-560058, Karnataka (IN)

DOI:

https://doi.org/10.55779/nsb14411317

Keywords:

GA3, HMGR1, Nicotiana tabacum, transcription run-on, transcriptional rate and mRNA stability

Abstract

Our present study evaluated the underlying molecular-mechanism(s) associated with the observed enhanced transcript levels and concomitant functional activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase 1 (NtHMGR1), a rate-limiting enzyme of cytosolic mevalonate (MVA) pathway of terpenoids biosynthesis, by gibberellin A₃ (GA₃) treatment in model cultivated tobacco, Nicotiana tabacum L. Based on the transcription run-on and cordycepin chase assays, our results demonstrated that tobacco seeds-priming with GA₃causes a relative and significantly enhanced transcriptional rate and mRNA stability of NtHMGR1. Taken together, our study established that GA₃mediated transcriptional and post-transcriptional regulatory control as one of the mechanisms for the observed enhanced transcript-levels and consequently enhanced functional activity of NtHMGR1.

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References

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