Effects of elicitors on secondary metabolite (SM) production and antioxidant activity in sweet basil (Ocimum basilicum L.) cell suspension cultures

  • Ebru BATI AY Amasya University, Suluova Vocational School, Department of Plant and Animal Production, 05500, Amasya (TR)
  • Beril KOCAMAN Amasya University, Suluova Vocational School, Department of Plant and Animal Production, 05500, Amasya (TR)
  • Emel KARACA ÖNER Ordu University, Vocational School of Technical Sciences, Department of Plant and Animal Production, 52200, Ordu (TR)
  • Muhammed Akif AÇIKGÖZ Ordu University, Faculty of Agriculture, Department of Field Crops, 52200, Ordu (TR)
Keywords: elicitor treatments, in vitro culture systems, phenolic, terpenoid


Elicitor treatments play an important role in inducing some protective signal transmitter enzymes in cells and regulating phenylalanine ammonia-lyase (PAL) activity. The aim of this study was to determine the effects of elicitors [silver nitrate (AgNO3), salicylic acid (SA) and yeast extract (YE)], which were added individually or in combination to Ocimum basilicum L. cell suspension cultures, on the production and antioxidant activity of secondary metabolites (SMs). Calluses were obtained from the leaves of O. basilicum kept on Murashige and Skoog (MS) medium containing 0.5 mg/l kinetin (KIN) + 2.5 mg/l naphthalene-acetic acid (NAA) and cell suspension cultures were initiated. Then elicitors were applied to the cell suspension cultures individually or in combination, and cells were harvested at the end of the second, fourth, and eighth days. Compared with the control culture, the maximum rosmarinic and chicoric acid production was obtained at the end of the 4th day from SA (24 µM) + YE (80 mg/l) treatment as 20.19 mg/g DW (118%) and 7.55 mg/g DW (123%), respectively. The maximum biosynthesis of isoquercetin and rutin compared with the control culture was 3.88 mg/g DW [YE (80 mg/l)] with a 1.6-fold increase and 6.35 mg/g DW [YE (80 mg/l) + AgNO3 (6 µM)] with a 1.76-fold increase, respectively. Estragole and linalool's highest values compared with the control culture were 4.50 μg/g DW [AgNO3 (6µM) + SA (24 µM)] and 3.02 μg/g DW [SA (24 µM)], respectively. Results clearly show that the elicitor treatment could enhance the biosynthesis of phenolic compounds and terpenoid content in cell suspension cultures of O. basilicum and may be used for commercial supply in the future for therapeutic applications.


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Abraham F, Bhatt A, Keng CL, Indrayanto G, Sulaiman SF (2011). Effect of yeast extract and chitosan on shoot proliferation, morphology and antioxidant activity of Curcuma mangga in vitro plantlets. African Journal of Biotechnology 10(40):7787-7795. http://dx.doi.org/10.5897/AJB10.1261

Açıkgöz MA, Kara ŞM, Aruc C, Ay E (2017). Morphogenetic, ontogenetic and diurnal variability in antimicrobial activity of bitter fennel (Foeniculum vulgare Miller var. vulgare) essential oil. Indian Journal of Pharmacological Education 51(3):190-194. http://dx.doi.org/10.5530/ijper.51.3s.10

Açıkgöz MA (2019). Evaluation of phytochemical compositions and biological properties of Achillea gypsicola Hub‐Mor. at different phenological stages. Chemistry and Biodiversity 16(12):e1900373. https://doi.org/10.1002/cbdv.201900373

Açıkgöz MA, Kara ŞM, Aygün A, Özcan MM, Ay EB (2019). Effects of methyl jasmonate and salicylic acid on the production of camphor and phenolic compounds in cell suspension culture of endemic Turkish yarrow (Achillea gypsicola) species. Turkish Journal of Agriculture and Forestry 43(3):351-359. https://doi.org/10.3906/tar-1809-54

Açıkgöz MA (2020a). Determination of essential oil compositions and antimicrobial activity of Achillea gypsicola Hub.-Mor. at different plant parts and phenological stages. Journal of Essential Oil Research 32(4):331-437. https://doi.org/10.1080/10412905.2020.1750496

Açıkgöz MA (2020b). Establishment of cell suspension cultures of Ocimum basilicum L. and enhanced production of pharmaceutical active ingredients. Industrial Crops Production 148:112278. https://doi.org/10.1016/j.indcrop.2020.112278

Açıkgöz MA (2021). Effects of sorbitol on the production of phenolic compounds and terpenoids in the cell suspension cultures of Ocimum basilicum L.. Biologia 76:395-409. https://doi.org/10.2478/s11756-020-00581-0

Ahmed AF, Attia FA, Liu Z, Li C, Wei J, Kang W (2019). Antioxidant activity and total phenolic content of essential oils and extracts of sweet basil (Ocimum basilicum L.) plants. Food Science and Human Wellness 8:299-305. https://doi.org/10.1016/j.fshw.2019.07.004

Ali M, Hahn EJ, Paek KY (2007). Methyl jasmonate and salicylic acid induced oxidative stress and accumulation of phenolics in Panax ginseng bioreactor root suspension cultures. Molecules 12(3):607-621. https://doi.org/10.3390/12030607

Alkuwayti MA, El-Sherif F, Yap YK, Khattab S (2019). Foliar application of Moringa oleifera leaves extract altered stress-responsive gene expression and enhanced bioactive compounds composition in Ocimum basilicum. South African Journal of Botany 129:291-298. https://doi.org/10.1016/j.sajb.2019.08.001

Amarowicz R, Pegg RB, Rahimi-Moghaddan P, Barl B, Weil JA (2004). Free radical scavenging activity and antioxidant activity of selected plant species from the Canadian Prairies. Food Chemistry 84:551-562. https://doi.org/10.1016/S0308-8146(03)00278-4

Aye A, Jeon YD, Lee JH, Bang KS, Jin JS (2019). Anti-inflammatory activity of ethanol extract of leaf and leaf callus of basil (Ocimum basilicum L.) on RAW 264.7 macrophage cells. Oriental Pharmacy and Experimental Medicine 19:217-226. https://doi.org/10.1007/s13596-019-00372-2

Azeez H, Ibrahim K (2013). Effect of biotic elicitors on secondary metabolite production in cell suspensions of Hypericum triquetrifolium Turra. Bulletin of the University of Agricultural Sciences & Veterinary Medicine Cluj-Napoca. Horticulture 70(1):26-33. https://doi.org//10.15835/buasvmcn-hort:9264

Bais HP, Walker TS, Schweizer HP, Vivanco JM (2002). Root specific elicitation and antimicrobial activity of rosmarinic acid in hairy root cultures of Ocimum basilicum. Plant Physiology and Biochemistry 40(11):983-995. https://doi.org/10.1016/S0981-9428(02)01460-2

Baldim JL, Silveira JGF, Almeida AP, Carvalho PLN, Rosa W, Schripsema J, Chagas-Paula DA, Soares MG, Luiz JHH (2018). The synergistic effects of volatile constituents of Ocimum basilicum against foodborne pathogens. Industrial Crops Production 112:821-829. https://doi.org/10.1016/j.indcrop.2017.12.016

Bálintová M, Bruňáková K, Petijová L, Čellárová E (2019). Targeted metabolomic profiling reveals interspecific variation in the genus Hypericum in response to biotic elicitors. Plant Physiology and Biochemistry 135:348-358. https://doi.org/10.1016/j.plaphy.2018.12.024

Cai Z, Kastell A, Speiser C, Smetanska I (2013). Enhanced resveratrol production in Vitis vinifera cell suspension cultures by heavy metals without loss of cell viability. Applied Biochemistry and Biotechnology 171(2):330-340. https://doi.org/10.1007/s12010-013-0354-4

Cheng Q, He Y, Li G, Liu Y, Gao W, Huang L (2013). Effects of combined elicitors on tanshinone metabolic profiling and SmCPS expression in Salvia miltiorrhiza hairy root cultures. Molecules 18 (7):7473-7485. https://doi.org/10.3390/molecules18077473

Chong TM, Abdullah MA, Lai OM, Nor’Aini FM, Lajis NH (2005). Effective elicitation factors in Morinda elliptica cell suspension culture. Process Biochemistry 40(11):3397-3405. https://doi.org/10.1016/j.procbio.2004.12.028

Coelho J, Veiga J, Karmali A, Nicolai M, Pinto Reis C, Nobre B, Palavra A (2018). Supercritical CO2 extracts and volatile oil of basil (Ocimum basilicum L.) comparison with conventional methods. Separations 5(2):21. https://doi.org/10.3390/separations5020021

Coskun Y, Duran RE, Kilic S (2019). Striking effects of melatonin on secondary metabolites produced by callus culture of rosemary (Rosmarinus officinalis L.). Plant Cell, Tissue and Organ Culture 1-7. https://doi.org/10.1007/s11240-019-01605-7

Deepthi S, Satheeshkumar K (2016). Enhanced camptothecin production induced by elicitors in the cell suspension cultures of Ophiorrhiza mungos Linn. Plant Cell, Tissue and Organ Culture 124(3):483-493. https://doi.org/10.1007/s11240-015-0908-y

Deschamps C, Simon JE (2006). Terpenoid essential oil metabolism in basil (Ocimum basilicum L.) following elicitation. Journal of Essential Oil Research 18(6):618-621. https://doi.org/10.1080/10412905.2006.9699183

Dağlioğlu Y, Açikgöz MA, Özcan MM, Kara ŞM (2022). Impact of application of alumina oxide nanoparticles on callus induction, pigment content, cell damage and antioxidant enzyme activities in Ocimum basilicum. Journal of International Environmental Application and Science 17(1):22-33.

Dowom SA, Abrishamchi P, Radjabian T, Salami SA (2017). Enhanced phenolic acids production in regenerated shoot cultures of Salvia virgata Jacq. after elicitation with Ag+ ions, methyl jasmonate and yeast extract. Industrial Crops Production 103:81-88. https://doi.org/10.1016/j.indcrop.2017.03.043

Duran RE, Kilic S, Coskun Y (2019). Melatonin influence on in vitro callus induction and phenolic compound production in sweet basil (Ocimum basilicum L.). In Vitro Cellular & Developmental Biology-Plant 55(4):468-475. https://doi.org/10.1007/s11627-019-10006-6

El-Esawi MA, Elansary HO, El-Shanhorey NA, Abdel-Hamid AM, Ali HM, Elshikh MS (2017). Salicylic acid-regulated antioxidant mechanisms and gene expression enhance rosemary performance under saline conditions. Frontiers in Physiology 8:716. https://doi.org/10.3389/fphys.2017.00716

El-Kader EMA, Serag A, Aref MS, Ewais EE, Farag MA (2019). Metabolomics reveals ionones upregulation in MeJA elicited Cinnamomum camphora (camphor tree) cell culture. Plant Cell, Tissue and Organ Culture 137(2):309-318. https://doi.org/10.1007/s11240-019-01572-z

El-Salam MA, Mekky H, El-Naggar EMB, Ghareeb D, El-Demellawy M, El-Fiky F (2015). Hepatoprotective properties and biotransformation of berberine and berberrubine by cell suspension cultures of Dodonaea viscosa and Ocimum basilicum. South African Journal of Botany 97:191-195. https://doi.org/10.1016/j.sajb.2015.01.005

Fazal H, Abbasi BH, Ahmad N, Ali SS, Akbar F, Kanwal F (2016). Correlation of different spectral lights with biomass accumulation and production of antioxidant secondary metabolites in callus cultures of medicinally important Prunella vulgaris L. Journal of Photochemistry and Photobiology B: Biology 159:1-7. https://doi.org/10.1016/j.jphotobiol.2016.03.008

Gadzovska S, Maury S, Delaunay A, Spasenoski M, Hagège D, Courtois D, Joseph C (2013). The influence of salicylic acid elicitation of shoots, callus, and cell suspension cultures on production of naphtodianthrones and phenylpropanoids in Hypericum perforatum L. Plant Cell, Tissue and Organ Culture 113(1):25-39. https://doi.org/10.1007/s11240-012-0248-0

Georgiev MI, Kuzeva SL, Pavlov AI, Kovacheva EG, Ilieva MP (2007). Elicitation of rosmarinic acid by Lavandula vera MM cell suspension culture with abiotic elicitors. World Journal of Microbiology and Biotechnology 23(2):301-304. https://doi.org/10.1007/s11274-006-9214-5

Gonçalves S, Mansinhos I, Rodríguez-Solana R, Pérez-Santín E, Coelho N, Romano A (2019). Elicitation improves rosmarinic acid content and antioxidant activity in Thymus lotocephalus shoot cultures. Industrial Crops Production 137:214-220. https://doi.org/10.1016/j.indcrop.2019.04.071

Gupta R, Singh A, Kanaujia R, Kushwaha S, Pandey R (2018). Trichodermaharzianum ThU and its metabolites underscore alteration in essential oils of Ocimum basilicum and Ocimum sanctum. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences 88(1):219-227. https://doi.org/10.1007/s40011-016-0753-8

Hahn MG, Albersheim P (1978). Host-pathogen interactions: XIV. Isolation and partial characterization of an elicitor from yeast extract. Plant Physiology 62:107-111. https://doi.org/10.1104/pp.62.1.107

Hakkim FL, Shankar CG, Girija S (2007). Chemical composition and antioxidant property of holy basil (Ocimum sanctum L.) leaves, stems, and inflorescence and their in vitro callus cultures. Journal of Agricultural and Food Chemistry 55:9109-9117. https://doi.org/10.1021/jf071509h

Hegde V, Partap PS, Yadav RC, Baswana KS (2017). In vitro androgenesis in Capsicum (Capsicum annuum L.). International Journal of Current Microbiology and Applied Sciences 6(5):925-933. https://doi.org/10.20546/ijcmas.2017.605.102

Hussain AI, Anwar F, Sherazi STH, Przybylski R (2008). Chemical composition, antioxidant and antimicrobial activities of basil (Ocimum basilicum) essential oils depends on seasonal variations. Food Chemistry 108(3):986-995. https://doi.org/10.1016/j.foodchem.2007.12.010

Kang SM, Min JY, Kim YD, Karigar CS, Kim SW, Goo GH, Choi MS (2009). Effect of biotic elicitors on the accumulation of bilobalide and ginkgolides in Ginkgo biloba cell cultures. Journal of Biotechnology 139(1):84-88. https://doi.org/10.1016/j.jbiotec.2008.09.007

Karuppusamy S (2009). A review on trends in production of secondary metabolites from higher plants by in vitro tissue, organ and cell cultures. Journal of Medicinal Plants Research 3(13):1222-1239.

Kim YK, Li X, Xu H, Park NI, Uddin MR, Pyon JY, Park SU (2009). Production of phenolic compounds in hairy root culture of tartary buckwheat (Fagopyrum tataricum Gaertn). Journal of Crop Science and Biotechnology 12(1):53-57. https://doi.org/10.1007/s12892-009-0075-y

Kintzios S, Makri O, Panagiotopoulos E, Scapeti M (2003). In vitro rosmarinic acid accumulation in sweet basil (Ocimum basilicum L.). Biotechnology Letters 25(5):405-408. https://doi.org/10.1023/A:1022402515263

Koroch AR, Simon JE, Juliani HR (2017). Essential oil composition of purple basils, their reverted green varieties (Ocimum basilicum) and their associated biological activity. Industrial Crops Production 107:526-530. https://doi.org/10.1016/j.indcrop.2017.04.066

Krishnan SS, Siril EA (2018). Elicitor mediated adventitious root culture for the large-scale production of anthraquinones from Oldenlandia umbellata L. Industrial Crops Production 114:173-179. https://doi.org/10.1016/j.indcrop.2018.01.069

Krishnan V, Ahmad S, Mahmood M (2015). Antioxidant potential in different parts and callus of Gynura procumbens and different parts of Gynura bicolor. BioMed Research International. http://dx.doi.org/10.1155/2015/147909

Kubeš J, Tůmová L, Martin J, Vildová A, Hendrychová H, Sojková K (2014). The production of isoflavonoids in Genista tinctoria L. cell suspension culture after abiotic stressors treatment. Natural Product Research 28(24):2253-2263. https://doi.org/10.1080/14786419.2014.938336

Kuo CL, Agrawal DC, Chang HC, Chiu YT, Huang CP, Chen YL, Huang SH, Tsay HS (2015). In vitro culture and production of syringin and rutin in Saussurea involucrata (Kar. et Kir.)–an endangered medicinal plant. Botanical Studies 56(1):12. https://doi.org/10.1186/s40529-015-0092-8

Kwee EM, Niemeyer ED (2011). Variations in phenolic composition and antioxidant properties among 15 basil (Ocimum basilicum L.) cultivars. Food Chemistry 128(4):1044-1050. https://doi.org/10.1016/j.foodchem.2011.04.011

Laloue M, Courtois D, Manigault P (1980). Convenient and rapid fluorescent staining of plant cell nuclei with ‘33258’Hoechst. Plant Science Letters 17(2):175-179. https://doi.org/10.1016/0304-4211(80)90145-5

Lee J, Scagel CF (2009). Chicoric acid found in basil (Ocimum basilicum L.) leaves. Food Chemistry 115(2):650-656. https://doi.org/10.1016/j.foodchem.2008.12.075

Li GJ, Wang SC, Xia K, Zhou X (2003). Effect of yeast elicitor and salicylic acid on the fluctuation of phytohormone contents in Ti-transformed Salvia miltiorrhiza cell cultures. Plant Growth Regulation 39(1):27-32. https://doi.org/10.1023/A:1021838918280

Liu R, Li W, Sun LY, Liu CZ (2012). Improving root growth and cichoric acid derivatives production in hairy root culture of Echinacea purpurea by ultrasound treatment. Biochemical Engineering Journal 60:62-66. https://doi.org/10.1016/j.bej.2011.10.001

Marwat SK, Khan MS, Ghulam S, Anwar N, Mustafa G, Usman K (2011). Phytochemical constituents and pharmacological activities of sweet basil–Ocimum basilicum L. (Lamiaceae). Asian Journal of Chemistry 9:3773-3782.

Mathew R, Sankar PD (2011). Growth characteristics of cell suspension cultures for secondary metabolite production in Ocimum basilicum L., Ocimum sanctum L. and Ocimum gratissimum. Journal of Pharma Research 4(10):3323-3326. http://doi.org/

Mathew R, Sankar PD (2012). Effect of methyl jasmonate and chitosan on growth characteristics of Ocimum basilicum L., Ocimum sanctum L. and Ocimum gratissimum L. cell suspension cultures. African Journal of Biotechnology 11(21):4759-4766. https://doi.org/10.5897/AJB11.3183

Mathew R, Sankar PD (2014). Comparison of major secondary metabolites quantified in elicited cell cultures, non-elicited cell cultures, callus cultures and field grown plants of Ocimum. International Journal of Pharmacy and Pharmaceutical Sciences 6(2):102-106.

Monfort LEF, Bertolucci SKV, Lima AF, de Carvalho AA, Mohammed A, Blank AF, Pinto JEBP (2018). Effects of plant growth regulators, different culture media and strength MS on production of volatile fraction composition in shoot cultures of Ocimum basilicum. Industrial Crops Production 116:231-239. https://doi.org/10.1016/j.indcrop.2018.02.075

Moscatiello R, Baldan B, Navazio L (2013). Plant cell suspension cultures. Humana Press, Totowa, New Jersey https://doi.org/10.1007/978-1-62703-152-3_5

Mousavi M, Zaiter A, Becker L, Modarressi A, Baudelaire E, Dicko A (2019). Optimisation of phytochemical characteristics and antioxidative properties of Foeniculum vulgare Mill. seeds and Ocimum basilicum L. leaves superfine powders using new parting process. Phytochemical Analysis 31(2):154-163. https://doi.org/10.1002/pca.2875

Nadeem M, Abbasi BH, Garros L, Drouet S, Zahir A, Ahmad W, Giglioli-Guivarc’h N, Hano C (2018). Yeast-extract improved biosynthesis of lignans and neolignans in cell suspension cultures of Linum usitatissimum L. Plant Cell, Tissue and Organ Culture 135(2):347-355. https://doi.org/10.1007/s11240-018-1468-8

Nadeem M, Abbasi BH, Younas M, Ahmad W, Zahir A, Hano C (2019). LED-enhanced biosynthesis of biologically active ingredients in callus cultures of Ocimum basilicum. Photochemistry and Photobiology B: Biology 190:172-178. https://doi.org/10.1016/j.jphotobiol.2018.09.011

Namdeo AG (2007). Plant cell elicitation for production of secondary metabolites: A review. Pharmacognosy Review 1(1):69-79.

Nazir M, Tungmunnithum D, Bose S, Drouet S, Garros L, Giglioli-Guivarc’h N, Abbasi BH, Hano C (2019). Differential production of phenylpropanoid metabolites in callus cultures of Ocimum basilicum L. with distinct in vitro antioxidant activities and in vivo protective effects against UV stress. Journal of Agricultural and Food Chemistry 67(7):1847-1859. https://doi.org/10.1021/acs.jafc.8b05647

Nguyen PM, Kwee EM, Niemeyer ED (2010). Potassium rate alters the antioxidant capacity and phenolic concentration of basil (Ocimum basilicum L.) leaves. Food Chemistry 123(4):1235-1241. https://doi.org/10.1016/j.foodchem.2010.05.092

Pandey H, Pandey P, Singh S, Gupta R, Banerjee S (2015). Production of anti-cancer triterpene (betulinic acid) from callus cultures of different Ocimum species and its elicitation. Protoplasma 252(2):647-655. https://doi.org/10.1007/s00709-014-0711-3

Pandey P, Singh S, Banerjee S (2019). Ocimum basilicum suspension culture as resource for bioactive triterpenoids: yield enrichment by elicitation and bioreactor cultivation. Plant Cell, Tissue and Organ Culture 137(1):65-75. https://doi.org/10.1007/s11240-018-01552-9

Park W, Arasu M, Al-Dhabi N, Yeo S, Jeon J, Park JS, Lee SY, Park SU (2016). Yeast extract and silver nitrate induce the expression of phenylpropanoid biosynthetic genes and induce the accumulation of rosmarinic acid in Agastache rugosa cell culture. Molecules 21(4):426. https://doi.org/10.3390/molecules21040426

Petersen M, Simmonds MS (2003). Rosmarinic acid. Phytochemistry 62(2):121-125. https://doi.org/10.1016/S0031-9422(02)00513-7

Piras A, Gonçalves MJ, Alves J, Falconieri D, Porcedda S, Maxia A, Salgueiro L (2018). Ocimum tenuiflorum L. and Ocimum basilicum L., two spices of Lamiaceae family with bioactive essential oils. Ind. Crops Production 113:89-97. https://doi.org/10.1016/j.indcrop.2018.01.024

Pourianezhad F, Rahnama H, Mousavi A, Khosrowshahli M, Mafakheri S (2019). Effects of combined elicitors on parthenolide production and expression of parthenolide synthase (TpPTS) in Tanacetum parthenium hairy root culture. Plant Biotechnology Reports 13:211-218. https://doi.org/10.1007/s11816-019-00526-3

Ptak A, Morańska E, Saliba S, Zieliński A, Simlat M, Laurain-Mattar D (2017). Elicitation of galanthamine and lycorine biosynthesis by Leucojum aestivum L. and L. aestivum ‘Gravety Giant’plants cultured in bioreactor RITA®. Plant Cell, Tissue and Organ Culture 128(2):335-345. https://doi.org/10.1007/s11240-016-1113-3

Rao SR, Ravishankar GA (2002). Plant cell cultures: chemical factories of secondary metabolites. Biotechnology Advances 20(2):101-153. https://doi.org/10.1016/S0734-9750(02)00007-1

Reyes-Martínez A, Antunes-Ricardo M, Gutiérrez-Uribe J, del Socorro Santos-Díaz M (2019). Enhanced production and identification of antioxidants in in vitro cultures of the cacti Mammillaria candida and Turbinicarpus laui. Applied Microbiology and Biotechnology 103(6):2583-2595. https://doi.org/10.1007/s00253-019-09656-8

Roy A, Bharadvaja N (2019). Establishment of root suspension culture of Plumbago zeylanica and enhanced production of plumbagin. Industrial Crops Production 137:419-427. https://doi.org/10.1016/j.indcrop.2019.05.007

Sabir SM, Ahmad SD, Hamid A, Khan MQ, Athayde ML, Santos DB, Boligon DB, Rocha JBT (2012). Antioxidant and hepatoprotective activity of ethanolic extract of leaves of Solidago microglossa containing polyphenolic compounds. Food Chemistry 131(3):741-747. https://doi.org/10.1016/j.foodchem.2011.09.026

Sarkate A, Banerjee S, Mir JI, Roy P, Sircar D (2017). Antioxidant and cytotoxic activity of bioactive phenolic metabolites isolated from the yeast-extract treated cell culture of apple. Plant Cell ,Tissue and Organ Culture 130(3):641-649. https://doi.org/10.1007/s11240-017-1253-0

Siddiqui BS, Bhatti HA, Begum S, Perwaiz S (2012). Evaluation of the antimycobacterium activity of the constituents from Ocimum basilicum against Mycobacterium tuberculosis. Journal of Ethnopharmacology 144(1):220-222. https://doi.org/10.1016/j.jep.2012.08.003

Singh B, Sahu PM, Sharma RA (2017). Effect of elicitors on the production of pyrroloquinazoline alkaloids by stimulating anthranilate synthase activity in Adhatoda vasica Nees cell cultures. Planta 246(6):1125-1137. https://doi.org/10.1007/s00425-017-2757-x

Sivanandhan G, Selvaraj N, Ganapathi A, Manickavasagam M (2014). Enhanced biosynthesis of withanolides by elicitation and precursor feeding in cell suspension culture of Withania somnifera (L.) Dunal in shake-flask culture and bioreactor. PLoS One 9(8):e104005. https://doi.org/10.1371/journal.pone.0104005

Strazzer P, Guzzo F, Levi M (2011). Correlated accumulation of anthocyanins and rosmarinic acid in mechanically stressed red cell suspensions of basil (Ocimum basilicum). Journal of Plant Physiology 168(3):288-293. https://doi.org/10.1016/j.jplph.2010.07.020

Talebi M, Moghaddam M, Pirbalouti AG (2018). Methyl jasmonate effects on volatile oil compounds and antioxidant activity of leaf extract of two basil cultivars under salinity stress. Acta Physiologiae Plantarum 40(2):34. https://doi.org/10.1007/s11738-018-2611-1

Tavares ES, Lopes D, Bizzo HR, Lage CL, Leitão SG (2004). Kinetin enhanced linalool production by in vitro plantlets of Lippia alba. J. Essential Oil Research 16(5):405-408. https://doi.org/10.1080/10412905.2004.9698756

Trettel JR, Gazim ZC, Goncalves JE, Stracieri J, Magalhaes HM (2018). Effects of copper sulphate (CuSO4) elicitation on the chemical constitution of volatile compounds and the in vitro development of Basil. Scientia Horticulturae 234:19-26. https://doi.org/10.1016/j.scienta.2018.01.062

Tusevski O, Stanoeva JP, Markoska E, Brndevska N, Stefova M, Simic SG (2016). Callus cultures of Hypericum perforatum L. a novel and efficient source for xanthone production. Plant Cell, Tissue and Organ Culture 125(2):309-319. https://doi.org/10.1007/s11240-016-0951-3

Velioglu YS, Mazza G, Gao L, Oomah BD (1998). Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. Journal of Agricultural and Food Chemistry 46(10):4113-4117. https://doi.org/10.1021/jf9801973

Vlase L, Benedec D, Hanganu D, Damian G, Csillag I, Sevastre B, Mot AC, Dumitrescu RS, Tilea I (2014). Evaluation of antioxidant and antimicrobial activities and phenolic profile for Hyssopus officinalis, Ocimum basilicum and Teucrium chamaedrys. Molecules 19(5):5490-5507. https://doi.org/10.3390/molecules19055490

Wu CH, Murthy HN, Hahn EJ, Paek KY (2007). Enhanced production of caftaric acid, chlorogenic acid and cichoric acid in suspension cultures of Echinacea purpurea by the manipulation of incubation temperature and photoperiod. Biochemical Engineering Journal 36(3):301-303. https://doi.org/10.1016/j.bej.2007.02.024

Xiao Y, Gao S, Di P, Chen J, Chen W, Zhang L (2010). Lithospermic acid B is more responsive to silver ions (Ag+) than rosmarinic acid in Salvia miltiorrhiza hairy root cultures. Bioscience Reports 30(1):33-40. https://doi.org/10.1042/BSR20080124

Xu A, Zhan JC, Huang WD (2015). Effects of ultraviolet C, methyl jasmonate and salicylic acid, alone or in combination, on stilbene biosynthesis in cell suspension cultures of Vitis vinifera L. cv. Cabernet Sauvignon. Plant Cell, Tissue and Organ Culture 122(1):197-211. https://doi.org/10.1007/s11240-015-0761-z

Xu Y, Fan M, Ran J, Zhang T, Sun H, Dong M, Zhang Z, Zheng H (2016). Variation in phenolic compounds and antioxidant activity in apple seeds of seven cultivars. Saudi Journal of Biological Sciences 23(3):379-388. https://doi.org/10.1016/j.sjbs.2015.04.002

Yan Q, Shi M, Ng J, Wu JY (2006). Elicitor-induced rosmarinic acid accumulation and secondary metabolism enzyme activities in Salvia miltiorrhiza hairy roots. Plant Science 170(4):853-858. https://doi.org/10.1016/j.plantsci.2005.12.004

Yang Y, Pu X, Qu X, Chen F, Zhang G, Luo Y (2017). Enhanced production of camptothecin and biological preparation of N 1-acetylkynuramine in Camptotheca acuminata cell suspension cultures. Applied Microbiology and Biotechnology 101(10):4053-4062. https://doi.org/10.1007/s00253-017-8153-4

Zaker A, Sykora C, Gössnitzer F, Abrishamchi P, Asili J, Mousavi SH, Wawrosch C (2015). Effects of some elicitors on tanshinone production in adventitious root cultures of Perovskia abrotanoides Karel. Industrial Crops Production 67:97-102. https://doi.org/10.1016/j.indcrop.2015.01.015

Zhao J, Zhu WH, Hu Q (2001). Enhanced catharanthine production in Catharanthus roseus cell cultures by combined elicitor treatment in shake flasks and bioreactors. Enzyme and Microbial Technology 28(7-8):673-681. https://doi.org/10.1016/S0141-0229(01)00306-4

Zhao JL, Zhou LG, Wu JY (2010). Effects of biotic and abiotic elicitors on cell growth and tanshinone accumulation in Salvia miltiorrhiza cell cultures. Applied Microbiology and Biotechnology 87(1):137-144. https://doi.org/10.1007/s00253-010-2443-4

Zhao JL, Zou L, Zhang CQ, Li YY, Peng LX, Xiang DB, Zhao G (2014). Efficient production of flavonoids in Fagopyrum tataricum hairy root cultures with yeast polysaccharide elicitation and medium renewal process. Pharmacognosy Magazine 10(39):234. https://doi.org/10.1002/pca.287510.4103/0973-1296.137362

How to Cite
BATI AY, E., KOCAMAN, B., KARACA ÖNER, E., & AÇIKGÖZ, M. A. (2022). Effects of elicitors on secondary metabolite (SM) production and antioxidant activity in sweet basil (Ocimum basilicum L.) cell suspension cultures. Notulae Scientia Biologicae, 14(2), 11246. https://doi.org/10.55779/nsb14211246
Research articles
DOI: 10.55779/nsb14211246