Biogenesis of silver nanoparticles from the shoot extract of Delonix regia its characterization (UV–Vis spectroscopy and SEM) and evaluation for antimicrobial potential

Bhawana SHARMA; Afroz ALAM

doi:10.55779/nsb15111199

Authors

Bhawana SHARMA Banasthali Vidyapith, Department of Bioscience and Biotechnology, Rajasthan-304022 (IN)
Afroz ALAM Banasthali Vidyapith, Department of Bioscience and Biotechnology, Rajasthan-304022 (IN)

DOI:

https://doi.org/10.55779/nsb15111199

Keywords:

AgNPs, antimicrobial activity, Delonix regia, FTIR, SEM, XRD

Abstract

The current research work explores the production of silver nanoparticles using aqueous extracts of Delonix regia (Boj. ex Hook.) Raf. (Angiosperms; Fabaceae) shoots for the bioreduction of Ag metal and its antimicrobial activity. Fourier transform infrared spectroscopy (FTIR), Zeta potential, ultraviolet-visible spectroscopy, scanning electron microscopy (SEM), and X-ray diffraction (XRD) have been used to evaluate the produced silver nanoparticles (AgNPs). Both antibacterial and antifungal activity were examined against bacterial and fungal pathogens, viz., Escherichia coli and Bacillus subtilis, and fungal strains, viz., Fusarium oxysporum and Aspergillus niger. The presence of silver nanoparticles was observed by the color change, i.e., from pale yellow to dark brown. The zeta potential observed for the produced nanoparticle is -18mv. The SEM and XRD revealed the size of synthesized AgNPs, i.e., 35nm and SEM size lies in the range of 40-60 nm. UV-visible absorption spectra were found at wavelength 425 nm. The synthesized nanoparticles are cost-efficient and could be an alternative procedure for the peculiar production of nanoparticles and also act as potential antimicrobial agents.

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