Enhanced production of lupeol through elicitation in in vitro shoot cultures of snake grass (Clinacanthus nutans)

Somarajan L.  CHIPPY; Vijayakumar T.  ANJU VIJAY; Achuthan S.  HEMANTHAKUMAR; Padmesh P. PILLAI; Thankappan S.  PREETHA

doi:10.55779/nsb14411195

Authors

Somarajan L. CHIPPY University College, Department of Botany, Plant Tissue Culture Laboratory, Thiruvananthapuram-695034, Kerala (IN)
Vijayakumar T. ANJU VIJAY University College, Department of Botany, Plant Tissue Culture Laboratory, Thiruvananthapuram-695034, Kerala (IN)
Achuthan S. HEMANTHAKUMAR Biotechnology and Bioinformatics Division, Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram 695562, Kerala (IN)
Padmesh P. PILLAI Central University of Kerala, Department of Genomic Science, Kasaragod 671316 Kerala (IN)
Thankappan S. PREETHA University College, Department of Botany, Plant Tissue Culture Laboratory, Thiruvananthapuram-695034, Kerala (IN)

DOI:

https://doi.org/10.55779/nsb14411195

Keywords:

chitosan, Clinacanthus nutans, elicitors, HPTLC, lupeol; methyl jasmonate, yeast extract

Abstract

Clinacanthus nutans (Acanthaceae), generally known as ‘snake grass’, has diverse uses in customary system of herbal medicine. The species is endowed with various bioactive compounds exhibiting extensive pharmacological properties. The present investigation focused on elicitor-intervened in vitro shoot biomass cultivation and scale-up production of the anti-cancerous compound ‘lupeol’, one of the foremost constituents in this species. For the augmented production of lupeol, the shoot cultures were elicited with various concentrations of yeast extract (YE), chitosan and methyl jasmonate (MeJA). Maximum shoot biomass yield and production of lupeol was detected in MS medium supplemented with 1.0 mgl^-1 BA and 400 mgl^-1 YE. The petroleum ether extracts of selected samples upon TLC analysis proved R_f values corresponding to lupeol. HPTLC analysis revealed that the sample treated with YE displayed relatively higher amount (975.50 ng) of lupeol than the in vivo plant (713.69 ng). Hence the in vitro shoot culture system with elicitor (YE) treatment propose an appropriate method for the elevated synthesis of lupeol which can be scaled up via bio-reactor technology in doing so profiting the pharmaceutical appliances.

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