Cryopreservation of aromatic ginger Kaempferia galanga L. by encapsulation-dehydration

  • Thankappan S. PREETHA University of Kerala, Department of Botany, University College, Thiruvananthapuram 695034, Kerala (IN)
  • Achuthan S. HEMANTHAKUMAR University of Kerala, Biotechnology and Bioinformatics Division, Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram 695562, Kerala (IN)
  • Peringatulli N. KRISHNAN University of Kerala, Biotechnology and Bioinformatics Division, Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram 695562, Kerala (IN)
Keywords: cryopreservation, encapsulation-dehydration, Kaempferia galanga, monocot, preculture, shoot tips


Kaempferia galanga L. is an endangered multi-purpose medicinal plant in Family Zingiberaceae, the rhizomes of which are used for several ayurvedic formulations. Encapsulation-dehydration (ED) method was optimized for cryopreservation of shoot tips of K. galanga. Shoot tips (STs) bearing the apical meristem dissected from the established in vitro shoot cultures were preconditioned in MS+0.4 M sucrose prior to encapsulation in calcium alginate and the beads subsequently transferred to MS liquid+0.3 M sucrose for 3 days afterward dehydration inside the laminar airflow for 4 hours upon rapid freezing in LN and rapid thawing produced maximum 62.2% survival and 46.7% regeneration rates. Shoot regeneration was observed from the apical meristems exclusive of intermediary callus phase. The plantlets regenerated from cryopreserved STs transferred to the field were phenotypically analogous with the mother plant.


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How to Cite
PREETHA, T. S., HEMANTHAKUMAR, A. S., & KRISHNAN, P. N. (2021). Cryopreservation of aromatic ginger Kaempferia galanga L. by encapsulation-dehydration. Notulae Scientia Biologicae, 13(4), 11024.
Research articles
DOI: 10.15835/nsb13411024