Prophylactic effect of the aqueous extract of Pimpinella anisum on the behavior of Wistar rats exposed to mercury

  • Wafaa ARABI University of Dr. Moulay Tahar Saida, Faculty of Sciences, Biology Department, Laboratory of Biotoxicology, Pharmacognosy and Biological Recovery of Plants (LBPVBP) (DZ)
  • Khaled KAHLOULA University of Dr. Moulay Tahar Saida, Faculty of Sciences, Biology Department, Laboratory of Biotoxicology, Pharmacognosy and Biological Recovery of Plants (LBPVBP) (DZ)
  • Djallal E. H. ADLI University of Dr. Moulay Tahar Saida, Faculty of Sciences, Biology Department, Laboratory of Biotoxicology, Pharmacognosy and Biological Recovery of Plants (LBPVBP) (DZ)
  • Mostapha BRAHMI University of Dr. Moulay Tahar Saida, Faculty of Sciences, Biology Department, Laboratory of Biotoxicology, Pharmacognosy and Biological Recovery of Plants (LBPVBP) (DZ)
  • Narimane TAIBI University of Dr. Moulay Tahar Saida, Faculty of Sciences, Biology Department, Laboratory of Biotoxicology, Pharmacognosy and Biological Recovery of Plants (LBPVBP) (DZ)
  • Miloud SLIMANI University of Dr. Moulay Tahar Saida, Faculty of Sciences, Biology Department, Laboratory of Biotoxicology, Pharmacognosy and Biological Recovery of Plants (LBPVBP) (DZ)
Keywords: immobility time; learning; locomotor activity; mercury; neurobehavior; Pimpinella anisum

Abstract

The purpose of this study was to evaluate the prophylactic effect of Pimpinella anisum (green anis) on neurobehavioral status following mercury chloride intoxication during the developmental period. For this purpose, rats exposed to 100 mg/L of HgCl2 during the gestation and lactation period. A group of rats was treated with the anis extract for 15 days before becoming intoxicated with mercury. In contrast, one group was orally administered aqueous anis extract for 15 days after intoxication. The forced swimming test, the open field test and the Morris pool respectively recorded an increase in immobility time, a decrease in the number of cross-cells (p <0.001), (p <0.05) and an increase in latency (p <0.01), (p <0.001), (p <0.001) and decreased time spent in the target frame during the probe test (p <0.01) and increased latency in the visible test (p <0.01) in HgCl2 - exposed rats compared to control rats. However, preventive and curative aniseed-based treatment reduced the rate of depression, increased locomotor activity and improved learning performance. In conclusion, the aqueous extract of Pimpinella anisum could have a corrective effect on some neurological disorders caused by mercury.

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References

Al Mofleh IA, Alhaider AA, Mossa JS, Al-Soohaibani MO, Rafatullah S (2007). Aqueous suspension of anise “Pimpinella anisum” protects rats against chemically induced gastric ulcers. World Journal of Gastroenterology 13(7):1112.

Azevedo BF, Simões MR, Fiorim J, Botelho T, Angeli JK, Vieira JVA, Vassallo DV (2016). Chronic mercury exposure at different concentrations produces opposed vascular responses in rat aorta. Clinical and Experimental Pharmacology and Physiology 43(7):712-719. https://doi.org/10.1111/1440-1681.12578

Bekara A, Ait Hamadouche N, Kahloula K, Sadi N, Aoues A (2016). Etude phytochimique et activité antioxydante de l’extrait aqueux de Pimpinella anisum L. Algerian Journal of Natural Products 4(3):299-307.

Bensefa-Colas L, Andujar P, Descatha A (2011). Mercury poisoning. Revue de Médecine Interne 32(7):416-24. https://www.hal.inserm.fr/inserm-00616716

Bjørklund G, Dadar M, Mutter J, Aaseth J (2017). The toxicology of mercury: Current research and emerging trends. Environmental Research 159:545-554. https://doi.org/10.1016/j.envres.2017.08.051

Chehimi L, Roy V, Jeljeli M, Sakly M (2012). Chronic exposure to mercuric chloride during gestation affects sensorimotor development and later behaviour in rats. Behavioural Brain Research 234(1):43-50. https://doi.org/10.1016/j.bbr.2012.06.005

Dauge V, Rossignol P, Roques BP (1988). Comparison of the behavioural effects induced by administration in rat nucleus accumbens or nucleus caudatus of selective μ and δ opioid peptides or kelatorphan an inhibitor of enkephalin-degrading-enzymes. Psychopharmacology 96(3):343-352.

Dos Santos AA, Hort MA, Culbreth M, López-Granero C, Farina M, Rocha JB, Aschner M (2016). Methylmercury and brain development: A review of recent literature. Journal of Trace Elements in Medicine and Biology 38:99-107. https://doi.org/10.1016/j.jtemb.2016.03.001

El-Sayed MGA, Elkomy A, Samer S, ElBanna AH (2015). Hepatoprotective effect of Pimpinella anisum and Foeniculum vulgare against carbon tetrachloride induced fibrosis in rats. World Journal of Pharmacy and Pharmaceutical Sciences 4(6):78-88.

Fyad K, Cheriti A, Bourmita Y, Belboukhari N (2013). Variabilité de la composition chimique des huiles essentielles de Coriandrum sativum L. et Pimpinella anisum L. au cours de développement végétatif. PhytoChem & BioSub Journal 7(1):35-35.

Gorji A, Ghadiri MK (2001). History of epilepsy in Medieval Iranian medicine. Neuroscience & Biobehavioural Reviews 25(5):455-461. https://doi.org/10.1016/S0149-7634(01)00025-2

Grandjean P, Perez M (2008). Development neurotoxicity: implications of methylmercury research. International Journal of Environment and Health 2(3-4):417-428. https://doi.org/10.1504/IJEnvH.2008.020933

Grandjean P, Weihe P, White RF, Debes F (1998). Cognitive performance of children prenatally exposed to “safe” levels of methylmercury. Environmental Research 77(2):165-172. https://doi.org/10.1006/enrs.1997.3804

Grosman M, Picot A (2009). Facteurs environnementaux impliqués dans la maladie d’Alzheimer. Le mercure dentaire, probable déterminant majeur. Medecine & Longevite 1(1):12-21. https://doi.org/10.1016/j.mlong.2009.06.001

Grosman M, Picot A (2010). Risques liés à l’exposition prénatale et postnatale au mercure. Contribution à «Mercury INC1», Stockholm, pp 7-11.

Harada M (1995). Minamata disease: methylmercury poisoning in Japan caused by environmental pollution. Critical Reviews in Toxicology 25(1):1-24. https://doi.org/10.3109/10408449509089885

Hazelhoff MH, Bulacio RP, Chevalier A, Torres AM (2018). Renal expression of organic anion transporters is modified after mercuric chloride exposure: Gender-related differences. Toxicology Letters 295:390-396. https://doi.org/10.1016/j.toxlet.2018.07.016

Hosseinzadeh H, Tafaghodi M, Abedzadeh S, Taghiabadi E (2014). Effect of aqueous and ethanolic extracts of Pimpinella anisum L. seeds on milk production in rats. Journal of Acupuncture and Meridian Studies 7(4):211-216. https://doi.org/10.1016/j.jams.2013.10.004

Kahloula K, Adli DEH, Slimani M, Terras H, Achour S (2014). Effet de l’exposition chronique au nickel sur les fonctions neurocomportementales chez les rats Wistar pendant la période de développement. Toxicologie Analytique et Clinique 26(4):186-192. https://doi.org/10.1016/j.toxac.2014.09.056

Kahloula K, Slimani M, Adli DEH, Rachdi S, Boumediene D (2013). Neuro beneficial effects of Pimpinella anisum against lead exposure. International Journal of Green Pharmacy 7(1).

Kjellstrom T (1989). Physical and mental development of children with prenatal exposure to mercury from fish. Stage 2: Interviews and psychological tests at age 6. Report (3642), National Swedish Environmental Protection Board, Solna, Sweden.

Kosalec I, Pepeljnjak S, Kuštrak D (2005). Antifungal activity of fluid extract and essential oil from anise fruits (Pimpinella anisum L., Apiaceae). Acta Pharmaceutica 55(4):377-385. https://hrcak.srce.hr/19043

Langford N, Ferner RJ, Hum H (1999). Toxicity of mercury. 13(10):651-656.

Macheix JJ, Fleuriet A, Jay-Allemand C (2005). Les composés phénoliques des végétaux: un exemple de métabolites secondaires d'importance économique. PPUR presses polytechniques.

Porsolt RD, Le Pichon M, Jalfre ML (1977). Depression: a new animal model sensitive to antidepressant treatments. Nature 266(5604):730-732. https://doi.org/10.1038/266730a0

Rao MV, Purohit AR (2011). Neuroprotection by melatonin on mercury induced toxicity in the rat brain. Pharmacology & Pharmacy 2(04):375. https://doi.org/10.4236/pp.2011.24049

Samojlik I, Mijatović V, Petković S, Škrbić B, Božin B (2012). The influence of essential oil of aniseed (Pimpinella anisum L.) on drug effects on the central nervous system. Fitoterapia 83(8):1466-1473. https://doi.org/10.1016/j.fitote.2012.08.012

Shahamat Z, Abbasi-Maleki S, Motamed SM (2016). Evaluation of antidepressant-like effects of aqueous and ethanolic extracts of Pimpinella anisum fruit in mice. Avicenna Journal of Phytomedicine 6(3):322.

Soleo L, Urbano ML, Petrera V, Ambrosi L (1990). Effects of low exposure to inorganic mercury on psychological performance. Occupational and Environmental Medicine 47(2):105-109. http://dx.doi.org/10.1136/oem.47.2.105

Tepe AS, Tepe B (2015). Traditional use, biological activity potential and toxicity of Pimpinella species. Industrial Crops and Products 69:153-166. https://doi.org/10.1016/j.indcrop.2015.01.069

Vesterberg O, Alessio L, Brune D, Gerhardsson L, Herber R, Kazantzis G, Sabbioni E (1993). International project for producing reference values for concentrations of trace elements in human blood and urine-TRACY. Scandinavian Journal of Work, Environment & Health 19-26. https://www.jstor.org/stable/40966369

Published
2021-02-03
How to Cite
ARABI, W., KAHLOULA, K., ADLI, D. E. H., BRAHMI, M., TAIBI, N., & SLIMANI, M. (2021). Prophylactic effect of the aqueous extract of Pimpinella anisum on the behavior of Wistar rats exposed to mercury. Notulae Scientia Biologicae, 13(1), 10878. https://doi.org/10.15835/nsb13110878
Section
Research articles
CITATION
DOI: 10.15835/nsb13110878