Seasonal arbuscular mycorrhiza colonization dynamic displays genotype-specific pattern in Iris sibirica L.

  • Ioana CRIȘAN University of Agricultural Sciences and Veterinary Medicine, Faculty of Agriculture, 3-5 Manastur Street, 400372 Cluj-Napoca (RO) https://orcid.org/0000-0002-9486-527X
  • Andrei STOIE University of Agricultural Sciences and Veterinary Medicine, Faculty of Agriculture, 3-5 Manastur Street, 400372 Cluj-Napoca; University of Agricultural Sciences and Veterinary Medicine, Agro-Botanical Garden (CLA), 3-5 Manastur Street, 400372 Cluj-Napoca (RO)
Keywords: cultivar; flower; phenology; root; symbiosis; wild

Abstract

Arbuscular mycorrhiza (AM) is a widespread symbiotic association between plants and Glomeromycota fungi, that brings nutritional-derived benefits for phytobiont. Influence of plant breeding on arbuscular mycorrhiza susceptibility is a topic of current interest that can have many practical implications. Insights into whether new cultivars have a lower mycorrhizal potential, are critical for optimization of AM use. Aim of this research was to conduct a comparative assessment of AM colonization across a phenophase gradient in two Iris sibirica genotypes: one displaying the wild traits versus a modern reblooming cultivar with double flowers. Analysis showed that both Iris sibirica genotypes developed Paris-morphotype. Results indicated that on average the genotype with simple flowers had a higher AM colonization frequency (84.44±2.15) compared to the new cultivar with double flowers (52.22±6.09). Significant influence was exercised both by genotype (p<0.001) as well as by phenophase (p=0.0013), over colonization frequency. The genotypes displayed contrasting colonization dynamics: highest AM frequency level occurred in spring for the genotype with simple flowers, and in autumn for the one with double flowers. Results suggest that host metabolic state has regulating role over functionality of established AM-symbiotic association according to plant nutritional requirements, while fungi might also respond to increased or decreased carbon flux in the plant, associated with geophyte phenology.

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References

Brundrett MC, Tedersoo L (2018). Evolutionary history of mycorrhizal symbioses and global host plant diversity. New Phytologist 220:1108-1115. https://doi.org/10.1111/nph.14976

Boltenkov E, Artyukova E, Kozyrenko M, Erst A, Trias-Blasi A (2020). Iris sanguinea is conspecific with I. sibirica (Iridaceae) according to morphology and plastid DNA sequence data. PeerJ 8:e10088. https://doi.org/10.7717/peerj.10088

Cavagnaro T, Smith FA, Lorimer MF, Haskard KA, Ayling SM, Smith SE (2008). Quantitative development of Paris-type arbuscular mycorrhizas formed between Asphodelus fistulosus and Glomus coronatum. New Phytologist 149:105-113. https://doi.org/10.1046/j.1469-8137.2001.00001.x

Cluj-Napoca climate database (2019a). Retrieved 2020 October 20 from https://www.wunderground.com

Cluj-Napoca climate database (2019b). Retrieved 2020 October 20 from https://en.tutiempo.net/climate

Crișan I, Vidican R, Stoian V, Stoie A (2017). Wild Iris spp. from Romanian meadows and their importance for ornamental plant breeding. Romanian Journal of Grassland and Forage Crops 16:21-32. https://www.cabdirect.org/cabdirect/abstract/20183119145

Crișan I, Vidican R, Stoian V, Sandor M, Stoie A (2018). Arbuscular mycorrhizae of five summer geophytes from Cluj county. Scientific Papers Agronomy Series 61(1):61-66. http://www.uaiasi.ro/revagrois/PDF/2018-1/paper/11.pdf

Crișan I, Vidican R, Olar L, Stoian V, Morea A, Ștefan R (2019). Screening for changes on Iris germanica L. rhizomes following inoculation with arbuscular mycorrhiza using Fourier transform infrared spectroscopy. Agronomy 9:815. https://doi.org/10.3390/agronomy9120815

Dickson S, Smith FA, Smith SE (2007). Structural differences in arbuscular mycorrhizal symbioses: more than 100 years after Gallaud, where next? Mycorrhiza 17:375-393. https://doi.org/10.1007/s00572-007-0130-9

Dickson S (2004). The Arum–Paris continuum of mycorrhizal symbioses. New Phytologist 163:187-200. https://doi.org/10.1111/j.1469-8137.2004.01095.x

Giesemann P, Rasmussen HN, Liebel H., Gebauer G (2019). Discreet heterotrophs: green plants that receive fungal carbon through Paris-type arbuscular mycorrhiza. New Phytologist 226:960-966. https://doi.org/10.1111/nph.16367

Grey-Wilson C (1997). Series Sibericae. In: White B, Bowley M, Brearley C et al. (Eds). A guide to species irises their identification and cultivation. Cambridge University Press, Cambridge, United Kingdom, pp 142.

Hodge A, Helgason T, Fitter AH (2010). Nutritional ecology of arbuscular mycorrhizal fungi. Fungal Ecology 3(4):267-273. https://doi.org/10.1016/j.funeco.2010.02.002

Hohmann P, Messmer MM (2017). Breeding for mycorrhizal symbiosis: focus on disease resistance. Euphytica 213:113. https://doi.org/10.1007/s10681-017-1900-x

Index Seminum (2020). Hortus Agro-Botanicus Napocensis, Academic Pres, pp 7-8.

INRA (2001). Mycocalc software. National Institute for Agricultural Research Dijon. Retrieved 2020 October 22 from https://www2.dijon.inrae.fr/mychintec/Mycocalc-prg/download.html

Jacott CN, Murray JD, Ridout CJ (2017). Trade-offs in arbuscular mycorrhizal symbiosis: disease resistance, growth responses and perspectives for crop breeding. Agronomy 7:75. https://doi.org/10.3390/agronomy7040075

Kaššák P (2013). Screening of presence of the chosen anthocyanin colorants in the Limniris group Irises. Preliminary Communication of 8th Croatian & 8th International Symposium on Agriculture, Dubrovnik, Croatia, pp 383-387. http://sa.agr.hr/pdf/2013/sa2013_p0409.pdf

Kaššák P, Kuli M (2014). Dyeing potential of the Iris sibirica L. flowers. 2nd Annual International Interdisciplinary Conference, Azores, Portugal. AIIC Proceedings 2:372-380. http://www.eujournal.org/index.php/esj/article/view/4163/3999.

Kokkoris V, Hamel C, Hart MM (2019). Mycorrhizal response in crop versus wild plants. PLoS One 14(8): e0221037. https://doi.org/10.1371/journal.pone.0221037

Konvalinková T, Püschel D, Řezáčová V, Gryndlerová H, Jansa J (2017). Carbon flow from plant to arbuscular mycorrhizal fungi is reduced under phosphorus fertilization. Plant and Soil 419(1-2):319-333. https://doi.org/10.1007/s11104-017-3350-6

Kovalev V, Mykhailenko O, Krechun A, Osolodchenko T (2017). Antimicrobial activity of extracts of Iris hungarica and Iris sibirica. Annals of the Mechnikov Institute 2:57-64.

Liu A, Ku YS, Contador CA, Lam HM (2020). The Impacts of domestication and agricultural practices on legume nutrient acquisition through symbiosis with rhizobia and arbuscular mycorrhizal fungi. Frontiers in Genetics 11:583954. https://doi.org/10.3389/fgene.2020.583954

NGA (2020). The National Gardening Association. Plant Data Base. Retrieved 2020 October 23 from https://garden.org/plants/view/232413/Siberian-Iris-Iris-Concord-Crush/

Tawaraya K. (2003). Arbuscular mycorrhizal dependency of different plant species and cultivars. Soil Science and Plant Nutrition 49(5):655-668. https://doi.org/10.1080/00380768.2003.10410323

Tejeda A, Zurita F (2020). Capacity of two ornamental species (Iris sibirica and Zantedeschia aethiopica) to take up, translocate, and accumulate carbamazepine under hydroponic conditions. Water 12:1272. https://doi.org/10.3390/w12051272

Trouvelot A, Kough JL, Gianinazzi-Pearson V (1986). Estimation of vesicular arbuscular mycorrhizal infection levels. Proceedings of the 1st European Symposium on Mycorrhizae, Dijon, France.

Ven A, Verlinden MS, Verbruggen E, Vicca S (2019). Experimental evidence that phosphorus fertilization and arbuscular mycorrhizal symbiosis can reduce the carbon cost of phosphorus uptake. Functional Ecology 33(11):2215-2225. https://doi.org/10.1111/1365-2435.13452

Vierheilig H, Coughlan AP, Wyss URS, Piché Y (1998). Ink and vinegar, a simple staining technique for arbuscular-mycorrhizal fungi. Applied and Environmental Microbiology 64:5004-5007. https://doi.org/10.1128/AEM.64.12.5004-5007.1998

Wang Y, Lv N, Mao X, Yan Z, Wang J, Tan W, Li X, Liu H, Wang L, Xi B (2018). Cadmium tolerance and accumulation characteristics of wetland emergent plants under hydroponic conditions. RSC Advances 8:33383-90. https://doi.org/10.1039/c8ra04015j

Yang J, Qi Y, Li H, Xu G (2017). Comparison of nitrogen and phosphorus purification effects of different wetland plants on eutrophic water. ICAESEE IOP Conf. Series: Earth and Environmental Science 113:012042. https://iopscience.iop.org/article/10.1088/1755-1315/113/1/012042

Zhu Y-G, Smith SE, Barritt AR, Smith FA (2001). Phosphorus (P) efficiencies and mycorrhizal responsiveness of old and modern wheat cultivars. Plant and Soil 237(2):249-255. https://doi.org/10.1023/a:1013343811110

Zubek S, Nobis M, Błaszkowski J, Mleczko P, Nowak A (2011). Fungal root endophyte associations of plants endemic to the Pamir Alay Mountains of Central Asia. Symbiosis 54:139-149. https://doi.org/10.1007/s13199-011-0137-z

Published
2021-01-19
How to Cite
CRIȘAN, I., & STOIE, A. (2021). Seasonal arbuscular mycorrhiza colonization dynamic displays genotype-specific pattern in Iris sibirica L. Notulae Scientia Biologicae, 13(1), 10838. https://doi.org/10.15835/nsb13110838
Section
Research articles
CITATION
DOI: 10.15835/nsb13110838