Synthesis and antimicrobial activities of a metallic oxide nanoparticle complex of Moringa oleifera leaves extracts against selected microorganisms

  • Mercy O. BAMIGBOYE University of Ilorin, Faculty of Physical Sciences, Department of Industrial Chemistry, Ilorin
  • Adeyinka E. AJIBOYE Kwara State University, Faculty of Pure and Applied Sciences, Department of Biosciences and Biotechnology, Microbiology Unit, Malete, Kwara State
Keywords: aqueous; ethanolic; extract; manganese oxide; Moringa oleifera; nanoparticle

Abstract

This research work aimed at synthesizing and investigating the antimicrobial activities of a metallic oxide nanoparticle complex of Moringa oleifera leaves extracts against some microorganisms. Moringa oleifera leaves were washed, dried and blended. They were extracted with distilled water and ethanol using standard methods. The nanoparticle was synthesized by coordinating with manganese oxide. The physicochemical properties were determined following standard procedures. The phytochemical screening was carried out by standard methods. The antibacterial activities were done using agar well diffusion method. Antifungal activity was carried out following the plate technique. The leaves extract had a 75% yield and melting point of 116 °C while the nanoparticle had a yield of 60% and melted at 78 °C with pH of 3.46. The molar conductance of the nanoparticle revealed at 10.6 Ω−1cm2mol−1. The ethanolic extract of the leaves showed the presence of alkaloids, tannin, steroids and saponins. The ethanolic extract of M. oleifera exhibited the highest antibacterial activity of 33.05±0.10 mm against Bacillus subtilis while its antifungal activity revealed the highest inhibition of 48.40±0.53 mm at 30 mg/mL against Aspergillus niger. Staphylococcus aureus had a zone of inhibition of 19.00±0.16a using the aqueous extract. The ethanolic extract of M. oleifera nanoparticles showed antibacterial and antifungal activity against B. megaterium and A. niger with a zone of inhibition of 49.21±0.32 mm and 50.35±0.29 mm respectively while the aqueous extract showed antibacterial activity against S. aureus with a zone of 26.00±0.38mm. As it was concluded ethanolic extract in both leaves extract and its nanoparticle, possessed higher antibacterial and antifungal activities than the aqueous extract.

Metrics

Metrics Loading ...

References

Abdallah EM, Khalid AS, Ibrahim N (2009). Antibacterial activity of oleo-gum resins of Commiphora molmol and Boswellia papyrifera against methicillin resistant Staphylococuss aureus (MRSA). Scientific Research and Essays 4(4):351-356.

Ahmed RN, Sani A, Ajiboye AE, Gambari-Ambali RO, Ezekiel K (2013). Sensitivity of three gastro-intestinal organisms to aqueous extract of leaf of Ocimum gratissimum. Nigeria Journal of Pure and Applied Sciences 26:2460-2469.

Ahmed RN, Sani A, Igunnugbemi OO (2009). Antifungal profiles of extracts of Vitellaria paradoxa (Shea-Butter) bark. Ethnobotanical Leaflets 6:2.

Airaodion AI, Ngwogu KO, Ngwogu AC, Ekenjoku JA (2019). Investigation of antibacterial activity of Vernonia amygdalina leaf extracts against gram-positive and gram-negative bacteria. International Journal of Bio-Science and Bio-Technology 11(11):87-93.

Alzoreky NS, Nakahara K (2003). Antibacterial activity of extracts from some edible plants commonly consumed in Asia. International Journal of Food Microbiology 80:223-230. https://doi.org/10.1016/S0168-1605(02)00169-1

Aruljothi S, Uma C, Sivagurunathan P, Bhuvaneswari M (2014). Investigation on antibacterial activity of Carica papaya leaf extracts against wound infection-causing bacteria. International Journal of Research Studies in Biosciences 2(11):8-12.

Azwanida NN (2015). A review on the extraction methods use in medicinal plants, principle, strength and limitation. Medicinal and Aromatic Plants 4(196):2167-0412. https://doi.org/10.4172/2167-0412.1000196

Bamigboye MO, Ahmed RN (2019). Comparative antimicrobial activities of a consortium of Vernonia amygdalina and Amaranthus hybridus extracts with their CuO nanoparticle complexes. International Journal of Medical Review 6(1):31-34. https://doi.org/10.29252/IJMR-060107

Banskota AH, Tezuka Y, Kadota S (2001). Recent progress in pharmacological research of propolis. Phytotherapy Research 15(7):561-571. https://doi.org/10.1002/ptr.1029

Barnabas CG, Nagarajan S (1988). Antimicrobial activity of flavonoids of some medicinal plants. Fitoterapia 3:508-510.

Blanco JG, Gil RR, Bocco JL, Meragelman TL, Genti-Raimondi S, Flury A (2001). Aromatase inhibition by an 11,13-dihydroderivative of a sesquiterpene lactone. Journal of Pharmacology and Experimental Therapeutics 297(3):1099-1105.

Cagin T, Che J, Qi Y, Zhou Y, Demiralp E, Gao G, Goddard WA (1999). Computational materials chemistry at the nanoscale. Journal of Nanoparticle Research 1:51-69. https://doi.org/10.1023/A:1010009630519

Castro SBR, Leal CA, Freire FR, Carvalho DA, Oliveira DF, Figueiredo HCP (2008). Antibacterial activity of plant extracts from Brazil against fish pathogenic bacteria. Brazilian Journal of Microbiology 39:756-760. https://doi.org/10.1590/S1517-838220080004000030

Cowan MM (1999). Plant products as antimicrobial agents. Clinical Microbiology Review 12:564-582. https://doi.org/10.1128/CMR.12.4.564

Daoud A, Malika D, Bakari S, Hfaiedh N, Mnafgui K, Kadri A (2019). Assessment of polyphenol composition, antioxidant and antimicrobial properties of various extracts of date palm pollen (DPP) from two Tunisian cultivar. Arabian Journal of Chemistry12(8):3075-3086. https://doi.org/10.1016/j.arabjc.2015.07.014 3075-3086

Doddanna SJ, Patel S, Sundarrao MA, Veerabhadrappa RS (2013). Antimicrobial activity of plant extracts on Candida albicans: an in vitro study. Indian Journal of Dental Research 24:401-405. https://doi.org/10.4103/0970-9290.118358

Enemose EA, Akporhonor EE, Kpomah B (2018). Preparation and evaluation of mixed-ligand complexes of Cu (II) and Co (II) with amodiaquine hydrochloride and sulphamethazine. Journal of Applied Sciences and Environmental Management 22(6):933-936. https://doi.org/10.4314/jasem.v22i6.16

Ezeifeka GO, Orji MU, Mbata TI, Patrick AO (2004). Antimicrobial activities of Cajanus cajan, Garcinia kola and Xylopia aethiopica on pathogenic microorganisms. Biotechnology 3(1):41-43. https://doi.org/10.3923/biotech.2004.41.43

Jana NR, Gearheart L, Murphy CJ (2001). Wet chemical synthesis of silver nanorods and nanowires of controllable aspect ratioElectronic supplementary information (ESI) available: UV-VIS spectra of silver nanorods. Chemical Communications 7:617-618.

Jayaraman SK, Manoharan MS, Illanchezian S (2008). Antibacterial, antifungal and tumor cell suppression potential of Morinda citrifolia fruit extracts. International Journal of Integrative Biology 3:44-49.

Karaman I, Sahin F, Gulluce M, Ogutcu H, Sengul M, Adiguzel A (2003). Antimicrobial activity of aqueous and methanol extracts of Juniperus oxycedrus L. Journal of Ethnopharmacology 85(2-3):231- 235. https://doi.org/10.1016/s0378-8741(03)00006-0

Kensa VM, Neelamegam R (2014). Evaluation of haematological properties of normal Albino rats exposed to ethanolic extract of Hydrilla verticillata (L.F.) collected from unpolluted and polluted water sources. International Journal of Current Microbiology and Applied Sciences 3(12):409-416.

Mabhiza D, Chitemerere T, Mukanganyama S (2016). Antibacterial properties of alkaloid extracts from Callistemon citrinus and Vernonia adoensis against Staphylococcus aureus and Pseudomonas aeruginosa. International Journal of Medicinal Chemistry. https://doi.org/10.1155/2016/6304163

Maitera ON, Louis H, Barminas JT, Akakuru OU, Boro G (2018). Synthesis and characterization of some metal complexes using herbal flavonoids. National Products Chemistry and Research 6:314. https://doi.org/10.4172/2329-6836.1000314

Mau JL, Chen CP, Hsieh PC (2001). Antimicrobial effect of extracts from Chinese chive, cinnamon, and corni fructus. Journal of Agricultural and Food Chemistry 49:183-188. https://doi.org/10.1021/jf000263c

Oda K, Matsuda H, Murakami T, Katayama S, Ohgitani T, Yoshikawa M (2000). Adjuvant and haemolytic activities of 47 saponins derived from medicinal and food plants. Biological Chemistry 381(1):67-74. https://doi.org/10.1515/BC.2000.009

Pal S, Tak YK, Song JM (2007). Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the Gram-negative bacterium, Escherichia coli. Applied Environmental Microbiology 73(6):1712-1720. https://doi.org/10.1128/aem.02218-06

Pirtarighat S, Ghannadnia M, Baghshahi S (2019). Green synthesis of silver nanoparticles using the plant extract of Salvia spinosa grown in vitro and their antibacterial activity assessment. Journal of Nanostructure in Chemistry 9(1):1-9. https://doi.org/10.1007/s40097-018-0291-4

Prabhu S, Poulose EK (2012). Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects. International Nano Letters 2(1):32. https://doi.org/10.1186/2228-5326-2-32

Ramakrishna D, Pragna R (2011). Nanoparticles: is toxicity a concern? The Journal of the International Federation of Clinical Chemistry and Laboratory Medicine 22(4):92-101.

Sharma AK, Gangwar M, Tilak R, Nath G, Sinha ASK, Tripathi YB, Kumar DC (2012). Comparative in vitro antimalarial and phyto-chemical evaluation of methanolic extract of root, stem and leaf of Jatropha curcas Linn. Pharmacognosy Journal 4(30):34-40. https://doi.org/10.5530/pj.2012.30.7

Wei LS, Musa N, Sengm CT, Wee W, Shazili NAM (2008). Antimicrobial properties of tropical plants against 12 pathogenic bacteria isolated from aquatic organisms. African Journal of Biotechnology 7(13):2275-2278. https://doi.org/10.5897/AJB08.915

Wong ST, Lee JF, Chen JM, Mou CY (2001). Preparation and characterization of MCM-41 and silica supported nickel boride catalysts. Journal of Molecular Catalysis A: Chemical 165(1-2):159-167. https://doi.org/10.1016/s1381-1169(00)00409-x

Yakubu MT, Adesokan AA, Akanji MA (2006). Biochemical changes in the liver, kidney and serum of rat following chronic administration of cimetidine. African Journal of Biomedical Research 9(3):213-218. https://doi.org/10.4314/ajbr.v9i3.48908

Published
2020-09-29
How to Cite
BAMIGBOYE, M. O., & AJIBOYE, A. E. (2020). Synthesis and antimicrobial activities of a metallic oxide nanoparticle complex of Moringa oleifera leaves extracts against selected microorganisms. Notulae Scientia Biologicae, 12(3), 619-627. https://doi.org/10.15835/nsb12310780
Section
Research articles