Young capitulum as important explant in in vitro mass propagation of gerbera (Gerbera jamesonii)

  • Budi WINARTO Central Java Assessment Institute for Agriculture Technology, Jl. Soekarno-Hatta KM 26, No. 10, Tegalsari, Bergas Lor, Bergas, Semarang 50552, Central Java
  • Kurnia YUNIARTO Indonesian Ornamental Crops Research Institute, Cianjur, Jl. Raya Ciherang, Pacet-Cianjur 43253, West Java
  • Rudy SOEHENDI Indonesian Ornamental Crops Research Institute, Cianjur, Jl. Raya Ciherang, Pacet-Cianjur 43253, West Java
Keywords: acclimatization; capitulum; gerbera clones; initiation; media; regeneration

Abstract

A new route of in vitro propagation of gerbera selected clones was successfully established using young capitula in tight buds and buds that were started to unfold stage as explant source. The one-fourth pieces of young capitula of tight flower stage and half-strength MS medium containing 0.25 mg/l BAP was the suitable for initiation and produced higher number of shoots per explant up to 3.8 shoots. The results were improved by culturing the one-fourth piece of 01.092 capitulums on MS medium fortified by 0.2 mg/l BAP and 0.02 mg/l NAA producing the highest shoot formation up to 8.5 shoots per explant with 28.7 leaves per explant and 2.1 cm leaf length. High multiple shoots were determined in third to fourth subculture periods and reduced thereafter with high multiplication rate noted on 01.092 clone. Shoots were easily rooted on half-strength MS medium supplemented with 2 g/l activated charcoal. Plantlets were transferred to ex vitro condition with 96.4% survivability of 03.045 clone using Cycas rumphii bulk and cocopeat (1:1, v/v) under spraying 1 g/l Growmore (32N:10P:10K) solution once week periodically. The route has high potential applied in qualified plantlet production for other Gerbera’s due to high shoots produced up to 35 shoots per whole young capitulum used. 

Metrics

Metrics Loading ...

References

Agrowindo (2015). Peluang Usaha Budidaya Bunga Gerbera dan Analisa Usahanya [Online]. Retrieved on 15 December 2019 from http://www.agrowindo.com/peluang-usaha-budidaya-bunga-gerbera-dan-analisa-usahanya.html

Altaf N, Khan AR, Ali L, Bhatti IA (2009). Tissue culture of gerbera. Pakistan Journal of Botany 41(1):7-10.

Badan Pusat Statistik dan Direktorat Jenderal Hortikultura (2019a). Luas Panen Nasional Tanaman Hias, 2013-2017 [Online]. Retrieved 15 December 2019 from https://www.pertanian.go.id/home/?show=page&act=view&id=61

Badan Pusat Statistik dan Direktorat Jenderal Hortikultura (2019b). Produksi Nasional Tanaman Hias, 2013-2017 [Online]. Retrieved 15 December 2019 from https://www.pertanian.go.id/home/?show=page&act=view&id=61

Badan Pusat Statistik dan Direktorat Jenderal Hortikultura (2019c). Produktivitas Nasional Tanaman Hias, 2013-2017 [Online]. Retrieved 15 December 2019 from https://www.pertanian.go.id/home/?show=page&act=view&id=61

Bhargava B, Dilta BS, Gupta YC, Dhiman SR, Modgil M (2013). Studies on micropropagation of gerbera (Gerbera jamesonii Bolus). Indian Journal of Applied Research 3(11):8-11.

Bhatia B, Singh KP, Singh MC (2012). In vitro mass multiplication of gerbera (Gerbera jamesonii) using capitulum explant. Indian Journal of Agricultural Sciences 82(9):768-774.

Cardoso JC, Teixeira da Silva JA (2013). Gerbera micropropagation. Biotechnology Advances 31:1344-1357.

Deshmukh G, Jhade RK, Alawa SL (2019). Economic feasibility of gerbera (Gerbera jamesonii L.) under protected cultivation with special reference to Chhindwara district of Madhya Pradesh. International Journal of Chemical Studies 7(2):1765-1768.

Gamanagatti P, Patil B (2018). Economic evaluation of protected cultivation technology (PCT) for horticulture crops. 30th International Conference of Agricultural Economists. July 28-August 2, 2018. Vancouver-Canada pp 18.

González YGH, Niño CYP (2014). Flowers capitulum disinfection for in vitro propagation of Gerbera jamesonii Bolus. Revista de la Facultad de Agronomía (LUZ) 31:510-523.

Hasbullah NA, Saleh A, Taha RM (2011). Establishment of somatic embryogenesis from Gerbera jamesonii (Bolus ex. Hook f) through suspension culture. African Journal of Biotechnology 10(63):13762-13768.

Hasbullah NA, Lassim MM, Azis NA, Daud NF, Rasad FM, Amin MAM (2015). Somatic embryo formation in Gerbera jamesonii Bolus ex. Hook f. in vitro. International Conference on Agricultural, Ecological and Medical Sciences (AEMS-2015) April 7-8, 2015 Phuket (Thailand) pp 25-28. http://dx.doi.org/10.15242/IICBE.C0415036

Hussein GM, Ismail IA, Hashem MES, Miniawy SME, Abdallah NA (2008). In vitro regeneration of gerbera. Agriculture and Forestry Research 58:97-102.

Izza-Florist. 2019. Daftar Harga [Online]. Retrieved 13 December 2019 from http://izzaflorist.com/daftar-harga

Kadu AR (2013). In vitro micropropagation of gerbera using auxiliary bud. Asian Journal of Bio Science 8(1):15-18.

Kanwar JK, Kumar S (2008). In vitro propagation of Gerbera - A review. Horticulture Science (Prague)35(1):35-44.

Lavakumaran L, Seran TH (2014). Effect of 6-benzyl-aminopurine and thidiazuron on in vitro shoot organogenesis of Aloe vera (L.) Burm. f. Chilean Journal of Agricultural Research 74(4):497-452.

Manjula R, Jholgiker P, Subbaiah KV, Prabhuling G, Swamy GSK, Kumar YL (2014). Morphological abnormality among hardened shoots of banana cv. ‘Rajapuri (AAB)’ after in vitro multiplication with TDZ and BAP from excised shoot tips. International Journal of Agriculture, Environment & Biotechnology 7(3):465-470.

Mattjik AA, Sumertajaya IS (2006). Experimental design with SAS and Minitab application. Indonesian: Perancangan Percobaan dengan Aplikasi SAS dan Minitab, pp 276.

Mishra SJ, Chandra R, Prasad L, Patel RK (2014). Influence of some phytohormones based culture medium on in vitro multiplication of Gerbera (Gerbera jamesonii). HortFlora Research Spectrum 3(1):65-68.

Mohamed SA, Özzambak ME (2014). Shoot regeneration capacity of in vitro cultures of some Gerbera (Gerbera jamesonii Bolus) explants. Sudanese Journal of Agricultural Sciences 1:24-29.

Murashige T, Skoog F (1962). A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiologia Plantarum 15:473-497.

Nazari F, Khosh-Khui M, Azadi P, Salehi H, Niazi A (2014). Growth regulators affected in vitro propagation of pot gerbera (Gerbera jamesonii cv. Royal Soft Pink). International Journal of Agriculture and Biosciences 3(4):185-189.

Naz S, Naz F, Tariq A, Aslam F, Ali A, Athar M (2012). Effect of different explants on in vitro propagation of gerbera (Gerbera jamesonii). African Journal Biotechnology 11(37):9048-9053.

Niedz RP, Hyndman SE, Evens TJ, Weathersbee AA (2014). Mineral nutrition and in vitro growth of Gerbera hybrida (Asteraceae). In Vitro Cell and Development of Biology-Plant 50:458-470.

Ranjan S, Gaurav S (2005). Somatic embryogenesis in gerbera (Gerbera jamesonii Bolus ex Hooker f.) as Influenced by explants. Journal of Ornamental Horticulture 8(2):128-130.

Shabanpour K, Sharifi A, Bagheri A, Moshtaghi N (2011). Effect of genotypes and culture medium on shoot regeneration and proliferation of Gerbera jamesonii. African Journal of Biotechnology 10(57):12211-12217.

Shirani S, Mahdavi F, Maziah M (2009). Morphological abnormality among regenerated shoots of banana and plantain (Musa spp.) after in vitro multiplication with TDZ and BAP from excised shoot tips. African Journal of Biotechnology 8(21):5755-5761.

Son NV, Mokashi AN, Hegde RV, Patil VS, Lingaraju S (2011). Response of gerbera (Gerbera jamesonii Bolus) varieties to micropropagation. Karnataka Journal of Agriculture Science 24(3):354-357.

Swetha TN, Girwani A, Rao AM, Saidaiah P (2017). Studies on tissue culture in gerbera (Gerbera jamesonii L.). International Journal of Agriculture Sciences. 9(17):162-165.

Tokopedia (2019). Bunga Potong Gerbera 1 Ikat 10 Batang [Online]. Retrieved 14 December 2019 from https://www.tokopedia.com/9845/bunga-potong-gerbera-1-ikat-10-batang

Umkm-news (2016). Menanam Bunga Gebera, Labanya Bikin Gembira [Online]. Retrieved 14 December 2019 from http://umkmnews.com/inspirasi/11760.html

Vijayalakshmi CL, Babu P, Bagali AN, Soregaon CD, Wadageri AH (2019). Direct in vitro regeneration of gerbera (Gerbera jamesonii Bolus). International Journal of Current Microbiology and Applied Sciences. 8(1):2610-2619. https://doi.org/10.20546/ijcmas.2019.801.274

Vinoth A, Ravindhran R (2018). In vitro morphogenesis of woody plants using thidiazuron. In: Ahmad N, Faisal M (Eds). Thidiazuron: From Urea Derivative to Plant Growth Regulator pp 211-221. https://doi.org/10.1007/978-981-10-8004-3_10

Winarto B, Yufdy MP (2017). Establishment of in vitro propagation protocol of Gerbera jamesonii Bolus Ex Hook F.: explant and media selection to plantlet acclimatization. Agraarteadus: Journal of Agricultural Science 28(1):32-40.

Winarto B, Yuniarto K, Wegadara M (2019). Axillary shoots derived from thin cell layer and adenine sulphate application in in vitro mass propagation of gerbera [Gerbera jamesonii (H. Bolus ex Bolus f.)]. Notulae Scientia Biologiae 11(1):63-76. https://doi.org/10.15835/nsb11110351

Published
2020-06-29
How to Cite
WINARTO, B., YUNIARTO, K., & SOEHENDI, R. (2020). Young capitulum as important explant in in vitro mass propagation of gerbera (Gerbera jamesonii). Notulae Scientia Biologicae, 12(2), 264-276. https://doi.org/10.15835/nsb12210712
Section
Research articles