Anticancer Activity of Water and Methanol Extracts of Hypericum scabrum L. on Different Cancer Cell Lines

  • Gulsen GUCLU Sivas Cumhuriyet University, Department of Health Care Services, Health Services Vocational School, Sivas
  • Ayca TAS Sivas Cumhuriyet University, Faculty of Health Sciences, Department of Nutrition and Diet, Sivas
  • Manhal TULIMAT Sivas Cumhuriyet University, Faculty of Medicine, Department of Medical Biochemistry, Sivas
  • Nuraniye ERUYGUR Selcuk University, Faculty of Pharmacy, Department of Pharmacognosy, Konya
  • Yavuz SILIG Sivas Cumhuriyet University, Faculty of Medicine, Department of Medical Biochemistry, Sivas
Keywords: cancer; cytotoxic; DU-145; HeLa; Hypericum scabrum; Saos-2

Abstract

Hypericum scabrum L. is an endemic medicinal plant with antimicrobial, anticancer and anti-inflammatory, antiviral effect. In this study, we aimed to investigate the cytotoxic effect of water and methanol extracts on different cancer cell lines such as osteosarcoma cancer, cervical cancer and prostate cancer. The data showed that the methanol extract had a highly effective cytotoxic effect on all three cell lines. Although water extract caused less cell loss than methanol extract, it was found that the cells were inhibited in all three cell lines. DU-145, which is the prostate cell line, was more effective in both extracts. As a result, the water and methanol extract of Hypericum scabrum L. have an anticancer effect on Saos-2, HeLa and DU-145 cells. There is a need for further and comprehensive studies by isolating the main active ingredient of this plant which is a candidate for drug of cancer in the future. Our study is expected to lead the studies in this direction.

Metrics

Metrics Loading ...

References

Aktuna AY (2018). Tendency of K562 chronic myeloid leukemia cells towards cell reprogramming. Turkish Journal of Hematology 35(4):260-264.

Baris D, Kizil M, Aytekin C, Kizil G, Yavuz M, Ceken B, Ertekin AS (2011).In vitro antimicrobial and antioxidant activity of ethanol extract of three Hypericum and three Achilleaspecies from Turkey. International Journal of Food Properties 4:339-355.

Baytop T (1999). Therapy with medicinal plants in Turkey (past and present). Istanbul University: Nobel Medicine Publications, (2 Ed.), Istanbul.

Borawska MH, Naliwajko SK, Moskwa J, Markiewicz-Żukowska R, Puścion-Jakubik A, Soroczyńska J (2016). Anti-proliferative and anti-migration effects of Polish propolis combined with Hypericum perforatum L. on glioblastoma multiforme cell line U87MG. BMC Complementary and Alternative Medicine 16(1):367.

Cakir A, Mavi A, Yıldırım A, Duru ME, Harmandar M, Kazaz C (2003). Isolation and characterization of antioxidant phenolic compounds from the aerial parts of Hypericum hyssopifolium L. by activity-guided fractionation. Journal of Ethnopharmacology 87(1):73-83.

Cirak C, Kurt D (2014). Onemli tıbbi bitkiler olarak Hypericum turleri. Anadolu Journal of Aegean Agricultural Research Institute 24:42-58.

Crockett SL, Robson NKB (2011). Taxonomy and chemotaxonomy of the genus Hypericum. Medicinal and Aromatic Plant Science and Biotechnology 5:1-13. Ebrahimzadeh MA, Nabavi SM, Nabavi SF, Ahangar N (2013). Anticonvulsant activity of Hypericum scabrum L: possible mechanism involved. European Review for Medical and Pharmacological Sciences 17(16):2141-2144.

Eruygur N, Ucar E, Akpulat HA, Shahsavari K, Safavi SM, Kahrizi D (2019). In vitro antioxidant assessment, screening of enzyme inhibitory activities of methanol and water extracts and gene expression in Hypericum lydium. Molecular Biology Reports 6(2):2121-2129.

Hamzeloo-Moghadam M, Khalaj A, Malekmohammad M (2015). Cytotoxic activity and apoptosis induction of Hypericum scabrumL. 336Iranian Red Crescent Medical Journal17(10): e19453.

Keser S, Keser F, Kaygili O, Tekin S, Demir E, Turkoglu I, ... Sandal S (2018). Phytochemical compounds and antiradical, antimicrobial, and cytotoxic activities of the extracts from Hypericum scabrumL. Flowers. Natural Product Research 2018:1-6.

Kizil G, Toker Z, Ozen HC, Aytekin C (2004). The antimicrobial activity of essential oils of Hypericum scabrum, Hypericum scabroidesand Hypericum triquetrifolium. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives 18(4):339-341.

Mahajna S, Kadan S, Tietel Z, Saad B, Khasib S, Tumeh A, ... Zaid H (2019). In vitro evaluation of chemically analyzed Hypericum triquetrifoliumextract efficacy in apoptosis induction and cell cycle arrest of the HCT-116 colon cancer cell line. Molecules 24(22):e4139.

Masters JRW(2000) Human cancer cell lines: Fact and fantasy. Nature Reviews Molecular Cell Biology 1(3):233-236. Neve RM, Chin K, Fridlyand J, Yeh J, Baehner FL, Fevr T, ... Tong F (2006). A collection of breast cancer cell lines for the study of functionally distinct cancer subtypes. Cancer Cell 10(6):515-527.

Ozturk N, Tuncel M, Potoglu-Erkara I (2009). Phenolic compounds and antioxidant activities of some Hypericumspecies: a comparative study with H. perforatum. Pharmaceutical Biology 47(2):120-127.

Serbetci T (2002). Hypericum scabrum L. turunun aktif bilesenleri yonunden degerlendirilmesi (in Turkish). Master Thesis, Istambul University, Saglik Bilimleri Enstitutusu, Instambul, Turkey.

Skehan P, Storeng R, Scudiero D, Monks A, McMahon J, ... Boyd MR (1990). New colorimetric cytotoxicity assay for anticancer-drug screening. Journal of the National Cancer Institute 82(13):1107-1112.

Stojanivic G, Dordevic A, Smelcerovic A (2013). Do other Hypericum species have medical potential as St. John’s wort (Hypericum perforatum)? Current Medicinal Chemistry 20(18):2273-2295.

Tahir NA, Azeez HA, Muhammad KA, Faqe SA, Omer DA (2017). Exploring of bioactive compounds in essential oil acquired from the stem and root derivatives of Hypericum triquetrifolium callus cultures. Natural Product Research 33(10):1504-1508.

Tanker N (1971). Hypericum scabrum L. hakkında. Journal of Faculty Pharmacy of Ankara University 1:10-15.

Turner T (2012). Development of the Polio vaccine: a historical perspective of Tuskegee University’s role in mass production and distribution of HeLa cells. Journal of Health Care for the Poor and Underserved 23(40):5-10.

Valletta E, Rinaldi A, Marini M, Franzese O, Roscetti G (2018). Distinct Hypericum perforatumL. total extracts exert different antitumor activity on erythroleukemic K562 cells. Phytotherapy Research 32(9):1803-1811.

Yesilada E, Honda G, Sezik E, Tabata M, Fujita T, Tanaka T, ... Takaishi Y (1995). Traditional medicine in Turkey. V. Folk medicine in the inner Taurus Mountains. Journal of Ethnopharmacology 46(3):133-152.

Zorzetto C, Sánchez-Mateo CC, Rabanal RM, Lupidi G,Petrelli D, Vitali L, ... Ricciutelli M (2015). Phytochemical analysis and in vitrobiological activity of three Hypericum species from the Canary Islands (Hypericum reflexum, Hypericum canariense and Hypericum grandifolium). Fitoterapia 100: 95-109.

Published
2019-12-24
How to Cite
GUCLU, G., TAS, A., TULIMAT, M., ERUYGUR, N., & SILIG, Y. (2019). Anticancer Activity of Water and Methanol Extracts of Hypericum scabrum L. on Different Cancer Cell Lines. Notulae Scientia Biologicae, 11(4), 333-336. https://doi.org/10.15835/nsb11410597
Section
Research articles