In vitro Plant Regeneration from Mature Seed Explants of Withania somnifera (L.) Dunal, an Important, Rare and Endangered Medicinal Plant

  • Sape Subba TATA Andhra University, Department of Botany, Visakhapatnam
  • Geddam JYOTHIRMAYEE Andhra University, Department of Botany, Visakhapatnam
  • Owk Aniel KUMAR Andhra University, Department of Botany, Visakhapatnam
Keywords: BAP; GA3; IAA; IBA; mature seed; Medharasayana

Abstract

Withania somnifera (L.) Dunal a member of the Solanaceae family, is a traditional medicinal plant commonly known in India as Ashwagandha. It is used for different diseases such as hiccup, cough, rheumatism, tuberculosis, and exhibits excellent antitumor and anti-bacterial activities as well. Direct organogenesis of plants using mature seeds provides faster response and is also a time saving approach, thus the present study was conducted to investigate the optimal concentrations and combinations of plant growth regulators with MS medium for the establishment of an efficient regeneration system in W. somnifera using mature seed as an explant. Therefore, an efficient in vitro protocol for high frequency regeneration has been developed using mature seeds as explant. In the present study, the multiple shoots along with embryogenic callus induction was best seen in MS medium supplemented with BAP (1.5 mg/L) and IAA (0.5 mg/L). Furthermore, MS medium fortified with GA3 (0.3 mg/L) and IBA (3.0 mg/L) alone was suited for shoot elongation and rhizogenesis respectively. The rooted plantlets were hardened and successfully established in the soil. The establishment of a highly reproducible regeneration system would greatly influence the efforts of improvement of the hereby studied medicinal plant species through useful gene transfer technology.

Metrics

Metrics Loading ...

References

Aasim M, Sahin-Demirbag N, Khawar KM, Kendir H, Ozcan S (2011). Direct axillary shoot regeneration from the mature seed explant of the hairy vetch (Vicia villosa Roth). Archives of Biological Sciences 63:757-762.

Al-Khayri JM, Huang FH, Morelock TE, Busharar TA (1992). In vitro plant regeneration of spinach from mature seed-derived callus. In Vitro-Plant 28(2):64-66.

Ashrafuzzaman M, Hossain M, Razi IM, Shahidul M, Haque M, Shahidullah SM, Shahin UZ (2009). Regeneration potential of seedling explants of chilli (Capsicum annuum). African Journal of Biotechnology 8:591-596.

Casado JP, Navarro MC, Utrilla MP, Martinez A, Jiménez J (2002). Micropropagation of Santolina canescens Lagasca and in vitro volatiles production by shoot explants. Plant Cell, Tissue and Organ Culture 69:147-153.

Chandran C, Karthikeyan K, Kulothungan S (2007). In vitro propagation of Withania somnifera (L.) Dunal. from shoot tip and nodal explants. Journal of Scientific Transactions in Environment and Technovation 1(1):15-18.

Devi PU, Sharada AC (1992). In vivo growth inhibitory effect of Withania somnifera (Ashwaganda) on a transplantable mouse tumour, Sarcoma 180. Indian Journal of Experimental Biology 30(3):169-172.

Devi PU (1996). Withania somnifera Dunal (Ashwaganda): potential plant source of a promising drug for cancer chemotherapy and radiosensitization. Indian Journal of Experimental Biology 34(10):927-932.

Fraternale D, Giamperi L, Ricci D, Rocchi MBL (2002). Micropropagation of Bupleurum fruticosum: the effect of triacontanol. Plant Cell, Tissue and Organ Culture 69:135-140.

Gupta PK, Pullman G, Timmis R, Kreitinger M, Carlson WC, Grob J, Wetty E (1993). Forestry in the 21st century: the biotechnology of somatic embryogenesis. Biotechnology 11(4):454- 459.

Joshi A, Kothari SL (2007). High copper levels in the medium improves shoot bud differentiation and elongation from the cultured cotyledons of Capsicum annuum L. Plant Cell, Tissue and Organ Culture 88:127-133.

Kiritikar KR, Basu BD (1975). Indian medicinal plants. Vol. 3. Bishen Singh Mahendra Pal Singh, Dehra Dun pp 1774-1777.

Murashige T, Skoog K (1962). A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiologia Plantarum 15(3):473-497.

Nadkarni AK (1976). Indian materia medica. Vol. 1. Popular Prakashan Pvt. Ltd. Bombay, Maharashtra, India pp 203-205.

Otroshy M, Moradi K, Nekouei MK, Struik PC (2011). Micropropagation of pepper (Capsicum annuum L.) through in vitro direct organogenesis. Asian Journal of Biotechnology 3:38-45.

Pacheco G, Gagliardi R, Carneiro L, Callado C, Valls J, Mansur E (2007). The role of BAP in somatic embryogenesis induction from seed explants of Arachis species from sections Erectoides and Procumbentes. Plant Cell, Tissue and Organ Culture 88:121-126.

Polisetty R, Paul V, Deveshwar JJ, Khetarpal S, Suresh K, Chandra R (1997). Multiple shoot induction by benzyladenine and complete plant regeneration from seed explants of chickpea (Cicer arietinum L.). Plant Cell Reports 16(8):565-571.

Qin C, Dong Z, Liu W, Deng Z, Tang L (2005). Effects of exogenous plant growth regulator on in vitro regeneration of cotyledon explants in pepper. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 33:25-32.

Rahman MT, Hossain MJ, Khalekuzzaman M (2008). In vitro indirect plantlet regeneration from hypocotyl segments and cotyledonary explant derived calli in lady’s finger (Abelmoschus esculentus Monech). Journal of Bio-Science 16:49-57.

Rani A, Kumar M, Kumar S (2014). In vitro propagation of Withania somnifera (L.) Dunal. from shoot apex explants. Journal of Applied and Natural Science 6(1):159-163.

Rout GR, Saxena C, Samantaray S, Das P (1999). Rapid plant regeneration from callus cultures of Plumbago zeylanica. Plant Cell, Tissue and Organ Culture 56:47-51.

Salvi ND, George L, Eapen S (2002). Micropropagation and field evaluation of micropropagated plants of turmeric. Plant Cell, Tissue and Organ Culture 68:143-151.

Sen J, Sharma AK (1991). Micropropagation of Withania somnifera from germinating seeds and shoot tips. Plant Cell, Tissue and Organ Culture 26:71-74.

Sharma N, Priya S, Manjul D, Eapen PK (2015). Comparative evaluation of in vitro regeneration potential of seeds of W. somnifera and W. coagulans. Biotechnology International 8(1):21-33.

Supe U, Dhote F, Roymon MG (2006). In vitro plant regeneration of Withania somnifera. Plant Tissue Culture and Biotechnology 16(2):111-115.

Williamson EM (2002). Major herbs of Ayurveda. Churchill Livingstone, London, UK pp 322-323.

Yang JL, Zhou CG, Zhao B, Jin CL, Zhang T, Li CH (2011). Rapid direct adventitious shoot organogenesis and plant regeneration from mature seed explants of Phellodendron amurense Rupr. Journal of Medicinal Plants Research 5(18):4560‐4565.

Published
2019-12-24
How to Cite
TATA, S. S., JYOTHIRMAYEE, G., & KUMAR, O. A. (2019). In vitro Plant Regeneration from Mature Seed Explants of Withania somnifera (L.) Dunal, an Important, Rare and Endangered Medicinal Plant. Notulae Scientia Biologicae, 11(4), 387-391. https://doi.org/10.15835/nsb11410512
Section
Research articles