Ethanolic Extract of Chrysophyllum albidum Stem Bark Prevents Alloxan-Induced Diabetes

  • Enoluomen Ben EHIGIATOR Madonna University Elele, Faculty of Pharmacy, Department of Pharmacology and Toxicology, Rivers State
  • Elias ADIKWU Niger Delta University Bayelsa State, Faculty of Pharmacy, Department of Pharmacology and Toxicology
Keywords: alloxan; Chrysophyllum albidum; diabetes; treatment; rat


Chrysophyllum albidum (C. albidum) is traditionally used for the treatment of diabetes, but there is a paucity of scientific evidence to support its use. This study investigated the effect of the ethanolic extract of Chrysophyllum albidum stem bark (EECA) on alloxan-induced diabetic rats. Normal and alloxan-induced diabetic rats were randomly divided into groups and treated with 100-200 mg/kg EECA for 7, 14 and 28 days respectively. Metformin (150 mg/kg) was used as the standard control. Blood samples were collected at the end of treatment for glucose test, while serum samples were extracted and assessed for high density lipoprotein (LDLC), triglyceride (TG) low density lipoprotein cholesterol (LDLC) and total cholesterol (TC). Pancreas was excised and evaluated for oxidative stress indexes. Blood glucose, serum TG, LDL-C and TC levels were significantly (p<0.001) increased whereas HDL-C levels were significantly (p<0.001) decreased in diabetic rats when compared to non-diabetic. Also, pancreatic malondialdehyde levels were significantly (p<0.001) increased whereas superoxide dismutase, glutathione, catalase, and glutathione peroxidase levels were significantly (p<0.001) decreased in diabetic rats when compared to non-diabetic control. However, alterations in the aforementioned parameters were reversed significantly in a dose and time-dependent fashion in diabetic rats treated with 100 mg/kg (p<0.05), 200 mg/kg (p<0.01) and 400 mg/kg (p<0.001) of EECA for 7, 14 and 28 days respectively when compared to diabetic control. EECA showed potential as remedy for diabetes which supports its use in folklore for the treatment of diabetes. 


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Abdel-Gayoum AG (2004). The effect of glycemic control in type 2 diabetic patients with diabetes-related dyslipidemia. Saudi Medical Journal 25(2):207-211.

Adisa SA (2000). Vitamin C, protein and mineral contents of African apple (Chrysophyllum albidum) In: Garba SA, Ijagbone IF, Iyagba AO, Olyamu AO, Kilani AS, Ufaruna N (Eds). Proceedings of the l8th Annual Conference of NIST pp 141-146.

Aebi H (1984). Catalase in vitro. Methods in Enzymology 105:121-126.

Akhtar MS, Nadeem M, Rashid B (2011). Hypoglycemic activity of different fractions of Chrysophyllum albidum root bark in normal and alloxan diabetic rats. Canada Journal of Applied Science 1(2):16-28.

Amusa N, Ashaye O, Oladapo M (2003). Biodeterioration of the African star apple (Chrysophyllum albidum) in storage and the effect on its food value. African Journal of Biotechnology 2(3):56-59.

Annapurna A, Kanaka MD, Murali KK (2001). Antidiabetic activity of a polyherbal preparation (tincture of punchparna) in normal and diabetic rats. Indian Journal of Experimental Biology 39: 500-502.

Buege JA, Aust SD (1978). Microsomal lipid peroxidation. Methods in Enzymology 52:302-310.

Dobrian AD, Davies MJ, Schriver SD, Lauterio TJ, Prewitt RL (2001). Oxidative stress in a rat model of obesity-induced hypertension. Hypertension 37(2):554-560.

Elsner M, Tiedge M, Guldbakke B, Munday R, Lenzen S (2002). Importance of the GLUT2 glucose transporter for pancreatic beta cell toxicity of alloxan. Diabetologia 45(11):1542-1549.

Ene AC, Nwankwo EA, Samdi LM (2007). Alloxan-induced diabetes in rats and the effects of black caraway (Carum carvi L.) oil on their body weight. Journal of Pharmacology and Toxicology 3(2):141-146.

Farombi EO (2003). African indigenous plants with chemotherapeutic potentials and biotechnological approach to the production of bioactive prophylactic agents. African. Journal of Biotechnology 2(12):662-671.

Friedewald WT, Levy RI, Fredrickson DS (1972). Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clinical Chemistry 18(6):499-502.

Gornall AG, Bardawill CJ, David MM (1949). Determination of serum proteins by means of the biureto reaction. Journal of Biological Chemistry 177(2):751-66.

Grover JK, Yadav S, Sats V (2002). Medicinal plants of India anti-diabetic potential. Journal of Ethnopharmacology 81(1):81-100.

Harborne IB (1998). Phytochemical methods: a guide to modern techniques of plant analysis, 3rd Edition. Springer Science & Business Media.

Harnett EM, Stratton RD, Browne RW, Rosner BA, Lanharm RJ, Armstrong D (2000). Serum markers of oxidative stress and severity of diabetic retinopathy. Diabetes Care 23(2):234-240.

Idowu TO, Iwalewa EO, Aderogba MA, Akinrelu BA, Ogundami AO (2006). Biochemical and behavioural effects of eleagnine from Chrysophyllum albidum. Journal of Biological Sciences 6(6):1029-1034.

Janić M, Volčanšek S, Lunder M, Janež A (2017). Metformin: from mechanisms of action to advanced clinical use Zdravniski Vestnik 86(3-4):138-157.

Kronenberg LM (2003). Williams textbook of endocrinology, 10th Edition. Elsevier India Publisher pp 1428-1431.

Lipinski B (2001). Pathophysiology of oxidative stress in diabetes mellitus. Journal of Diabetes its Complications 15(4):203-210.

Lorke D (1998). A new approach to practical acute toxicity testing. Archives of Toxicology 534(4):275-287.

Marriff HI, Al BH, Hassan KM (2005). Some pharmacological studies on Chrysophyllum africanum in rabbits and mice. Journal of Ethnopharmacology 49:51-55.

Mohini P, Subhash P, Manohar P, Abhijit T, Vijay N (2012). Effect of thespesone-vanadium complex in alloxan induced diabetic rats. African Journal of Pharmacy and Pharmacology 6(10):692-697.

Newman DJ, Cragg GM, Snader KM (2003). Natural products as sources of new drugs. Journal of Natural Products 66:1022-1033.

Njolstad PR, Sagen JV, Bjorkhaug L, Odili S, Shehadeh N, Bakry D, … Sarici SU (2003). Permanent neonatal diabetes caused by glucokinase deficiency: inborn error of the glucose-insulin signaling pathway. Diabetes 52(11):2854-2860.

Okoli B, Okere OS (2010). Antimicrobial activity of the phytochemical constituent of Chrysophyllum albidum G. Don plant. Journal of Research in National Development 8(1):35-42.

Olajide OA, Awe S, Makinde JM (2004). Purgative effect of the methanol extract of Moringa lucida. Fitoterapia 70(1):199-204.

Olorunnisola DS, Amao IS, Ehigie DU, Ajayi AT (2008). Anti-hyperglycemic and hypolipidemic effect of ethanolic extract of Chrysophyllum albidum seed cotyledon in alloxan induced diabetic rats. Research Journal of Applied Sciences 3(2):123-127.

Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG (1973). Selenium: biochemical role as a component of glutathione peroxidase. Science 179(4073):588-590.

Sarwar N, Gao P, Seshasai SR, Gobin R, Kaptoge S, Sattar N (2010). Diabetes mellitus fasting blood glucose concentration and risk of vascular disease: a collective meta-analysis of 102 prospective studies. The Lancet 375(9733):2215-2222.

Sedlak J, Lindsay RH (1968). Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Analyticsl Biochemistry 25:192-205.

Sun M, Zigma S (1978). An improved spectrophotometer assay of superoxide dismutase based on epinephrine antioxidation. Analytical Biochemistry 90(1):81-89.

Susan JL, Helseth LD (1997). Reducing the complications of type II diabetes: a patient-centered approach. American Family Physician 56(2):471-80.

World Health Organization (1985). Report of a WHO study group on definition, diagnosis and classification of diabetes mellitus. Technical Report Series 727:7-113.

How to Cite
EHIGIATOR, E. B., & ADIKWU, E. (2019). Ethanolic Extract of Chrysophyllum albidum Stem Bark Prevents Alloxan-Induced Diabetes. Notulae Scientia Biologicae, 11(3), 325-331.
Research articles