Molecular Characterization of Vitex agnus-castus L. (Verbenaceae) Populations Grown in Aydin, Turkey
In this study, we performed a genetic diversity analysis using RAPD markers for some Vitex agnus-castus populations grown in Aydin, Turkey. Total genomic DNA isolation from the leaves of Vitex agnus-castus was performed using a commercial kit. Seven RAPD primers (OPA-02, OPA-05, OPA-13, OPA-15, OPA-16, OPA-18, OPA-20) were used to determine genetic diversity among populations. Polymerase Chain Reaction (PCR) was performed with all genomic DNA samples and primers. PCR products were run in agarose gel electrophoresis and visualized under UV light. The amplified products were scored as bands (1) and no bands (0) for all gel images and their matrix files were generated. A total of 36 characters were obtained from the primers. Phylogenetic relationships and genetic distances between the cultivars were calculated by using the PAUP* (Phylogenetic Analysis Using Parsimony and other methods) program. According to PAUP analysis, the closest genetic distances were between Çine pink flower and Çakmar purple flower, and Çakmar pink flower and Çakmar purple flower populations with a value of 0.05556; and the greatest genetic distance was between Çakmar pink flower and Köşk purple flower populations with a value of 0.36111. In the phylogenetic analysis obtained using UPGMA algorithms, the phylogenetic tree consisted of four groups. The results suggest that RAPD markers are useful tools for determining genetic relationships among Vitex agnus-castus genotypes.
Arulbalachandran D, Mullainathan L, Karthigayan S, Somasundaram ST, Velu S. (2010). Genetic variation in mutants of black gram (Vigna mungo (L.) Hepper) evaluated by RAPD markers. Journal of Crop Science and Biotechnology 13(1):1-6.
Aydın SÖ (2004). RAPD (Rastgele Artırılmış Polimorfik DNA) belirleyicileri ile bitki sistematiği. Dumlupınar Üniversitesi Fen Bilimleri Enstitüsü Dergisi 6:113-130.
Baciu AD, Pamfil D, Mihalte L, Sestras AF, Sestras RE (2013). Phenotypic variation and genetic diversity of Calendula officinalis L. Bulgarian Journal of Agricultural Science 19(1):143-151.
Bardakçı F (2001). Random amplified polymorphic DNA (RAPD) markers. Turkish Journal of Biology 25(2):185-196.
Botez C, Sestras R, Ardelean M, Pamfil D, Patrascu B, Sestras A (2009). Phenotypic selection assisted by molecular markers for scab resistance in apple. Acta Horticulturae 814:771-776.
Casas AM, Igartua E, Balaguer G, Moreno M A (1999). Genetic diversity of Prunus rootstocks analyzed by RAPD markers. Euphytica 110(2):139-149.
Eryiğit T, Çig A, Okut N, Yıldırım B, Ekici K (2015). Evaluation of chemical composition and antimicrobial activity of Vitex agnus castus L. fruits’ essential oils from west Anatolia, Turkey. Journal of Essential Oil Bearing Plants 18(1):208-214.
Fakir H, Erbaş S, Özen M, Dönmez İE (2014). The effects of different harvest dates on essential oil content and composition in chaste Ttee (Vitex agnus-castus L.). European Journal of Science and Technology 1(2):25-28.
Hürkul MM, Köroğlu A (2018). Morphological and anatomical studies on hayıt fruit (fructus agni-casti) and leafs sold in the market. Biological Diversity and Conservation 11(2):47-59.
Hussein EH, Adawy SS, SEME Ismail, El-Itriby HA (2005). Molecular characterization of some Egyptian date palm germplasm using RAPD and ISSR markers. Arab Journal of Biotechnology 8(1):83-98.
Monte-Corvo L, Cabrita L, Oliveira C, Leitão J (2000). Assessment of genetic relationships among Pyrus species and cultivars using AFLP and RAPD markers. Genetic Resources and Crop Evolution 47(3): 257-265.
Nayak S, Rout GR, Das P (2003). Evaluation of the genetic variability in bamboo using RAPD markers. Plant Soil and Environment 49(1):24-28.
Olgun M, Budak Başçiftçi Z, Ayter NG, Turan M, Koyuncu O, Ardıç M, Ağar G, Takıl E (2015). Genetic divergence in some Barley (Hordeum vulgare L.) genotypes by RAPD and ISSR analyses. SDU Journal of the Faculty of Agriculture/SDÜ Ziraat Fakültesi Dergisi 10(2):102-109.
Özbek Ö, Gıdık BU (2013). Genetic diversity in commercial rapeseed (Brassica napus L.) varieties from Turkey as revealed by RAPD. Notulae Scientia Biologicae 5(1):114-119.
Poyraz İE, Sözen E, Ataşlar E, Poyraz İ (2012). Determination of genetic relationships among Velezia L. Caryophyllaceae) species using RAPD markers. Turkish Journal of Biology 36(3):293-302.
Rahman MO, Rahman MZ, Sony SK, Islam MN (2017). Genetic variation and molecular relationships among eight taxa of Desmodium Desv. based on RAPD markers. Bangladesh Journal of Plant Taxonomy 24(2):149-154.
Rice-Evans C, Miller N, Paganga G (1997). Antioxidant properties of phenolic compounds. Trends in Plant Science 2(4):152-159.
Sesli M, Yegenoglu ED (2017). Genetic relationships in wild olives (Olea europaea ssp. oleaster) by ISSR and RAPD markers. Biotechnology and Biotechnological Equipment 31(5): 897-904.
Stojković D, Soković M, Glamočlija J, Džamić A, Ćirić A, Ristić M, Grubišić D (2011). Chemical composition and antimicrobial activity of Vitex agnus-castus L. fruits and leaves essential oils. Food Chemistry 128(4):1017-1022.
Swofford DL (2002). PAUP. Phylogenetic Analysis Using Parsimony (and other methods). Version 4.0b10. Sinaeur Associates, Sunderland.
Williams JG, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18(22):6531-6535.
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