Arbuscular Mycorrhizal and Dark Septate Endophyte Fungal Association in Cassava (Manihot esculenta Crantz) Varieties, Southern India
The study examined the root characteristics, type of arbuscular mycorrhizal (AM) and dark septate endophyte (DSE) fungal associations and their extent of colonization in 13 widely cultivated cassava (Manihot esculenta Crantz) varieties in southern India. Root and associated soil samples of each cassava variety were collected from Salem and Kolli Hills of the Eastern Ghats in south India. In addition, we also investigated the relationship between the extent of colonization of AM, DSE fungal and AM fungal spore communities and soil characteristics for each cassava variety. Roots of all the cassava varieties were colonized by AM fungi and 12 varieties had co-colonization of DSE fungi. The AM morphology in all the cassava varieties was of an intermediate type with intercellular and intracellular linear hyphae and arbusculate coils. In addition, we found high AM colonization rates (73-89%) but only moderate rates by DSE (22-68%) fungi. We also found 17 AM fungal spore morphotypes and their abundance varied significantly among cassava varieties. In addition, different physicochemical properties of soils may possibly increase AMF diversity, while different varieties of cassava are commonly associated with differing levels of AM and DSE fungi colonization with soil factors potentially influencing the symbiosis.
Abbott LK, Murphy DV (2007). Biological soil fertility: A key to sustainable land use in agriculture. Springer Verlag, London, UK.
Akoroda MO, Oyinlola AE, Gebremeskel T (1987). Plantable stem supply system for IITA cassava varieties in Oyo State of Nigeria. Agricultural Systems 24(4):305-317.
Alguacil MM, Torrecillas E, Garcia-Orenes F, RoldÃ¡n A (2014). Changes in the composition and diversity of AMF communities mediated by management practices in a Mediterranean soil are related with increases in soil biological activity. Soil Biology and Biochemistry 76:34-44.
Begoude DAB, Sarr PS, Mpon TLY, Owona DA, Kapeua MN, Araki S (2016). Composition of arbuscular mycorrhizal fungi associated with cassava (Manihot esculenta Crantz) cultivars as influenced by chemical fertilization and tillage in Cameroon. Journal of Applied Biosciences 98:9270-9283.
Brush SB (1995). In situ conservation of landraces in centers of crop diversity. Crop Science 35(2):346-354.
Burns AE, Gleadow RM, Zacarias AM, Cuambe CE, Miller RE, Cavagnaro TR (2012). Variations in the chemical composition of cassava (Manihot esculenta Crantz) leaves and roots as affected by genotypic and environmental variation. Journal of Agricultural and Food Chemistry 60(19):4946-4956.
Cardoso IM, Kuyper TW (2006). Mycorrhizas and tropical soil fertility. Agriculture, Ecosystems and Environment 116(1-2):72-84.
Carrenho R, Trufem SFB, Bononi VLR, Silva ES (2007). The effect of different soil properties on arbuscular mycorrhizal colonization of peanuts, sorghum and maize. Acta Botanica Brasilica 21(3):723-730.
Carretero CL, Cantos M, Garcia JL, Azcon R, Troncoso A (2009). Growth response of micropropagated cassava clones as affected by Glomus intraradices colonization. Journal of Plant Nutrition 32(2):261-273.
Ceballos I, Ruiz M, Fernandez C, Pena R, Rodriguez A, Sanders IR (2013). The in vitro mass-produced model mycorrhizal fungus, Rhizophagus irregularis, significantly increases yields of the globally important food security crop cassava. PLoS One 8(8):70633.
Cordones MN, AlemÃ¡n F, MartÃnez V, Rubio F (2014). K+ uptake in plant roots. The systems involved their regulation and parallels in other organisms. Journal of Plant Physiology 171(9):688-695.
Dickson S (2004). The Arum-Paris continuum of mycorrhizal symbioses. New Phytologyst 163(1):187-200.
Dickson S, Smith FA, Smith SE (2007). Structural differences in arbuscular mycorrhizal symbioses: more than 100 years after Gallaud, where next? Mycorrhiza 17(5):375-393.
Duran C (2014). Relationship between rainfall distribution and physical geography elements within Mersin province, turkey. Procedia-Social and Behavioral Sciences 120:740-748.
Edison S, Anantharaman M, Srinivas T (2006). Status of cassava in India, an overall view. Technical Bulletin 46, Published by the Director, Central Tuber Crops Research Institute, Thiruvananthapuram, Kerala, India.
Figueiredo PG, Moraes-Dallaqua MA, Bicudo SJ, Tanamati FY, Aguiar EB (2015). Development of tuberous cassava roots under different tillage systems: descriptive anatomy. Plant Production Science 18(3):241-245.
Garcia K, Zimmermann SD (2014). The role of mycorrhizal associations in plant potassium nutrition. Frontiers in Plant Science 5:3-37.
Hammer y, Harper DA T, Ryan PD (2001). Paleontological statistics software: package for education and data analysis. Palaeontologia Electronica 4.
Herren HR, Bennett FD (1984). Biological control of cassava pests. In: Advancing agricultural production in Africa. Proceedings, CAB's Scientific Conference, edited by D.L. Hawksworth, pp 12-18.
Howeler RH (2002). Cassava mineral nutrition and fertilization. In Hillocks RJ, Thresh MJ and Bellotti AC.(eds) Cassava biology, production and utilization. CABI publishing CAB International, Wallingford, Oxon, UK.
Howler R, Lutaladio N, Thomas G (2013). Save and grow: Cassava - A guide to sustainable production intensification. Food and Agriculture Organization of the United States of America, Rome.
IMD (2013). Temperature of Salem District. Regional Meterological Centre, Chennai, India.
Jackson ML (1971). Soil Chemical Analysis. Prentice Hall, New Delhi, India.
Karthigairaj M, Kalaiarasu S (2015). Biodiversity of AM fungi in the cassava grown soils of semiarid tropics of Tamilnadu. Asian Journal of Science and Technology 6:1815-1818.
Khade SW, Rodrigues BF (2009). Arbuscular mycorrhizal fungi associated with varieties of Carica papaya L. in tropical agro-based ecosystem of Goa, India. Tropical and Subtropical Agroecosystem 10(3):369-381.
Khan MA, Gemenet DC, Villordon A (2016). Root system architecture and abiotic stress tolerance: current knowledge in root and tuber crops. Frontiers in Plant Science 7:1584.
Knapp DG, Pintye A, Kovacs GM (2012). The dark side is not fastidious - dark septate endophytic fungi of native and invasive plants of semiarid sandy areas. PLoS One 7(2):e32570.
Koske RE, Gemma JN (1989). A modified procedure for staining roots to detect VA mycorrhizas. Mycological Research 92(4):486-488.
Lebot V, Champagne A, Malapa R, Shirley D (2009). NIR determination of major constituents in tropical root and tuber crop flours. Journal of Agricultural and Food Chemistry 57(22):10539-10547.
Legris P, Meher-Homji VM (1984). The Eastern Ghats: phytogeographic aspects. International Journal of Life-Sciences Scientific Research 4:115-136.
Lendenmann M, Thonar C, Barnard R, Salmon Y, Werner R, â€¦ Jansa J (2011). Symbiont identity matters: carbon and phosphorus fluxes between Medicago truncatula and different arbuscular mycorrhizal fungi. Mycorrhiza 21(8):689-702.
Lindsay WL, Norvell WA (1978). Development of a DTPA soil test for Zn, Fe, Mn, and Cd. Soil Science Society of America Journal 42(3):421-428.
Liu C, Ravnskov S, Liu F, RubÃ¦k GH, Andersen MN (2018). Arbuscular mycorrhizal fungi alleviate abiotic stresses in potato plants caused by low phosphorus and deficit irrigation/partial rootzone drying. The Journal of Agricultural Science 156(1):46-58.
Liu W, Zhang Y, Jiang S, Deng Y, Christie P, Murray PJ, â€¦ Zhang J (2016). Arbuscular mycorrhizal fungi in soil and roots respond differently to phosphorus inputs in an intensively managed calcareous agricultural soil. Scientific Report 6:24902.
Ma Z, Bielenberg DG, Brown KM, Lynch JP (2001). Regulation of root hair density by phosphorus availability in Arabidopsis thaliana. Plant Cell Environment 24(4):459-467.
Magurran AE (2004). Measuring biological diversity. Oxford: Blackwell Science. United Kingdom. USA.
Maherali H (2014). There an association between root architecture and mycorrhizal growth response. New Phytologist 204(1):192-200.
Mandyam K, Jumpponen A (2005). Abundance and possible functions of the root-colonising dark septate endophytic fungi. Studies in Mycology 53:173-190.
Mandyam K, Loughin T, Jumpponen A (2010). Isolation and morphological and metabolic characterization of common endophytes in annually burned tall grass prairie. Mycologia 102(4):813-821.
Marschner H, Dell B (1994). Nutrient uptake in mycorrhizal symbiosis. Plant and Soil 159(1):89-102.
Mathimaran N, Ruh R, Jama B, Verchot L, Frossard E, Jansa J (2007). Impact of agricultural management on arbuscular mycorrhizal fungal communities in Kenya ferrasol. Agriculture, Ecosystems and Environment 119(1-2):22-23.
McGonigle TP, Miller MH, Evans DG, Fairchild GL, Swan JA (1990). A new method which gives an objective measure of colonization of roots by vesicular-arbuscular mycorrhizal fungi. New Phytologist 115(3):495-501.
Medina RD, Faloci MM, Gonzalez AM, Mroginski LA (2007). In vitro cultured primary roots derived from stem segments of cassava (Manihot esculenta Crantz) can behave like storage organs. Annals of Botany 99(3):409-423.
Muthukumar T, Tamilselvi V (2010). Occurrence and morphology of endorhizal fungi in crop species. Tropical and Subtropical Agroecosystems 12(3):593-604.
Muthukumar T, Udaiyan K (2000). Arbuscular mycorrhizas of plants growing in the Western Ghats region, Southern India. Mycorrhiza 9(6):297-313.
Newsham KK (2011). A meta-analysis of plant responses to dark septate root endophytes. New Phytologist 190(3):783-793.
Roldan ME, Arredondo JT, Huber-Sannwald E, Chapa-Vargas L, Portugal VO (2008). Grazing effects on fungal root symbionts and carbon and nitrogen storage in a short grass steppe in Central Mexico. Journal of Arid Environment 72(4):546-556.
Okon IE, Solomon MG, Osonubi O (2010). The effects of arbuscular mycorrhizal fungal inoculation and mulch of contrasting chemical composition on the yield of cassava under humid tropical conditions. The Scientific World Journal 10:505-511.
Ouimet R, Camire C, Furlan V (1996). Effect of soil K, Ca and Mg saturation and endomycorrhization on growth and nutrient uptake of sugar maple seedlings. Plant and Soil 179(2):207-216.
Oyetunji OJ, Osonubi O (2007). Assessment of influence of alley cropping system and arbuscular mycorrhizal (AM) fungi on cassava productivity in derived savanna zone of Nigeria. World Journal of Agricultural Science 3(4):489-495.
Peterson RL, Wagg C, Pautler M (2008). Associations between micro fungal endophytes and roots: do structural features indicate function? Botany 86(5):445-456.
Postma JW M, Olsson PA, Grerup UF (2007). Root colonization by arbuscular mycorrhizal, fine endophytic and dark septate fungi across a pH gradient in acid beech forests. Soil Biology and Biochemistry 39(2):400-408.
Reininger V, Grunig CR, Sieber TN (2012). Host species and strain combination determine growth reduction of spruce and birch seedlings colonized by root-associated dark septate endophytes. Environmental Microbiology 14(4):1064-1076.
Rogers D, Appan C (1973). Manihot, Manihotoides, Euphorbiaceae. Flora Neotropica Hafner Press, New York.
SchÃ¼ÃŸler A, Schwarzott D, Walker C (2001). A new fungal phylum, the Glomeromycota: phylogeny and evolution. Mycological Research 105(12):1413-1421.
SchÃ¼ÃŸler A, Walker C (2010). The Glomeromycota. A species list with new families and new genera: Published in December 2010 in libraries at The Royal Botanic Garden Edinburgh, The Royal Botanic Garden Kew, Botanische Staatssammlung Munich, and Oregon State University.
Smith FA, Smith SE (2011). What is the significance of the arbuscular mycorrhizal colonization of many economically important crop plants. Plant and Soil 348(1-2):63-79.
Smith SE, Read DJ (2008). Mycorrhizal Symbiosis.3rd edition. Academic Press. London.
Sreenivas T, Anantharaman M (2005). Cassava marketing systems in India. Tech Bull Series 43:1-162.
Straker CJ, Hilditch AJ, Rey ME C (2010). Arbuscular mycorrhizal fungi associated with cassava (Manihot esculenta Crantz) in South Africa. South African Journal of Botany 76(1):102-111.
Subramanian KS, Selvan TA (2001). Geology of Tamilnadu and Pondicherry. Geological Society of India, Bangalore.
Tchabi A, Hountondji FCC, Ogunsola B, Lawouin L, Coyne D, Wiemken A, Oehl F (2016). The influence of arbuscular mycorrhizal fungi inoculation on micro-propagated hybrid Yam (Dioscorea spp.) growth and root knot nematode (Meloidogyne sp.) suppression. IJCMAS 5:267-281.
Villordon AQ, Ginzberg I, Firon N (2014). Root architecture and root and tuber crop productivity. Trends in Plant Science 19(7):419-425.
Voko RR, Nandjui J, Seryjm D, Fotso B, Amo JA, Kouadi MSA, â€¦ Zeze A (2013). Abundance and diversity of arbuscular mycorrhizal fungal (AMF) communities associated with cassava (Manihot esculenta Crantz) rhizosphere in Abengourou. East Cote d Ivoire 5(11):360-370.
Willis AB, Rodrigues F, Harris PJC (2013). The ecology of arbuscular mycorrhizal fungi. Critical Reviews in Plant Sciences 32(1):1-20.
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