Zinc Influences Regeneration of Talinum portulacifolium Stem Cuttings in Nutrient Solution


  • Thangavelu MUTHUKUMAR Bharathiar University, Department of Botany, Root and Soil Biology Laboratory, Coimbatore 641046, Tamilnadu (IN)
  • Sarah JAISON Bharathiar University, Department of Botany, Root and Soil Biology Laboratory, Coimbatore 641046, Tamilnadu (IN)
  • Selvam Dinesh BABU Bharathiar University, Department of Botany, Root and Soil Biology Laboratory, Coimbatore 641046, Tamilnadu (IN)




growth; heavy metals; hydroponics; rooting


Zinc (Zn) is an essential micronutrient for plant growth and development, but toxic at high concentrations. The present study focused to underline the direct effect of different concentrations (0-50 ppm) of Zn on the regeneration ability and morphological characteristics of Talinum portulacifolium stem cuttings in hydroponic solution over a period of 35 days without the interference of other soil factors. High concentrations of Zn (40-50 ppm) affected callusing, root initiation, root and shoot development to varying levels. At high concentrations, Zn caused stem decay, stunting and browning of roots, wilting, withering and necrotic spots on leaves. Increasing concentrations of Zn inversely affected the lateral shoot development, stem elongation, leaf, root numbers and total root length of the stem cuttings. Though Zn had no significant influence on fresh or dry weights of stem, low concentration (15 ppm) of Zn increased the fresh and dry weights of leaves by 11.17% and 26.79% respectively, compared to 0 ppm and with 77.06-243.80% and 47.92-255.00% compared with those raised in 20-50 ppm. Zn concentrations >10 ppm reduced the root fresh weight by 28.57-90.47% and dry weight by 27.27-90.91% than those at 0 ppm. The Zn content in leaves and stems increased linearly with increasing concentrations of Zn and ranged from 1.09-125.62 ppm and 1.00-110.26 in stems and leaves respectively. The tolerance index varied between 81 and 138 for different concentrations of Zn. The results of the study clearly indicate that high concentrations of Zn inhibit the regeneration of T. portulacifolium stem cuttings.


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Agarwal K, Sharma A, Talukder G (1987). Copper toxicity in plant cellular systems. Nucleus 30(2):131-158.

Andrades-Moreno L, Cambrollé J, Figueroa ME, Mateos-Naranjo E (2013). Growth and survival of Halimione portulacoides stem cuttings in heavy metal contaminated soils. Marine Pollution Bulletin 75(1-2):28-32.

Antosiewicz DM (1993). Mineral status of dicotyledonous crop plants in relation to their constitutional tolerance to lead. Environmental and Experimental Botany 33(4):575-589.

Audet P, Charest C (2007). Heavy metal phytoremediation from a meta-analytical perspective. Environmental Pollution 147(1):231-237.

Babu RK, Vinay K, Sameena S K, Prasad S V, Swapna S, Rao AC (2009). Antihyperglycemic and antioxidant effects of Talinum portulacifolium leaf extracts in streptozotocin diabetic rats: A dose-dependent study. Pharmacognosy Magazine 5(9):1-10.

Balkhair KS, Ashraf MA (2016). Field accumulation risks of heavy metals in soil and vegetable crop irrigated with sewage water in western region of Saudi Arabia. Saudi Journal of Biological Sciences 23(1):S32-S44.

Baryla AP, Carrier F, Franck C, Coulomb C, Sahut M and Havaux (2001). Leaf chlorosis in oilseed rape plants (Brassica napus) grown on cadmium-polluted soil: causes and consequences for photosynthesis and growth. Planta 212(5-6):696-709.

Broadley MR, White PJ, Hammond JP, Zelko I, Lux A (2007). Zinc in plants. New Phytologist 173(4):677-702.

Castiglione S, Franchin C, Fossati T, Lingua G, Torrigiani P, Biondi S (2007). High zinc concentrations reduce rooting capacity and alter metallothionein gene expression in white poplar (Populus alba L. cv. Villafranca). Chemosphere 67(6):1117-1126.

Clemens S (2017). How metal hyperaccumulating plants can advance Zn biofortification. Plant and Soil 411(1-2):111-120.

Cobbett C, Goldsbrough P (2002). Phytochelatins and metallothioneins: roles in heavy metal detoxification and homeostasis. Annual Review of Plant Biology 53:159-182.

Conn SJ, Hocking B, Dayod M, Xu B, Athman A, Henderson S, Aukett L, Conn V, Shearer MK, Fuentes S, Tyerman SD, Gilliham M (2013). Protocol: optimising hydroponic growth systems for nutritional and physiological analysis of Arabidopsis thaliana and other plants. Plant Methods 9:4. https://doi.org/10.1186/1746-4811-9-4

Coolong TW, Randle WM, Toler HD, Sams CE (2004). Zinc availability in hydroponic culture influences glucosinolate concentrations in Brassica rapa. Hortscience 39 (1):84-86.

Das P, Samantaray S, Rout G (1997). Studies on cadmium toxicity in plants: a review. Environmental Pollution 98(1):29-36.

Das S, Green A (2013). Importance of zinc in crops and human health. Journal of SAT Agricultural Research 11:1-7.

Dos Santos Utmazian MN, Wieshammer G, Vega R, Wenzel WW (2007). Hydroponic screening for metal resistance and accumulation of cadmium and zinc in twenty clones of willows and poplars. Environmental Pollution 148(1):155-165.

Dushenkov V, Kumar PBAN, Motto H, Raskin I (1995). Rhizofiltration: the use of plants to remove heavy metals from aqueous streams. Environmental Science and Technology 29(5):1239-1245.

Fasani E, Manara A, Martini F, Furini A, DalCorso G (2018).The potential of genetic engineering of plants for the remediation of soils contaminated with heavy metals. Plant, Cell and Environment 41(5):1201-1232.

Hagemeyer J (1999). Ecophysiology of plant growth under heavy metal stress. In: Prasad MNV, Hagemeyer J (Eds.) Heavy metal stress in plants; from molecules to ecosystems. Springer-Verlag, Berlin Heidelberg pp 157-181.

Hassanein AMA (2013). Factors influencing plant propagation efficiency via stem cuttings. Journal of Horticultural Science and Ornamental Plants 5(3):171-176.

Hoagland DR, Arnon DI (1950). The water-culture method for growing plants without soil. College of Agriculture, University of California, Berkeley.

Iori V, Pietrini F, Massacci A, Zacchini M (2015). Morphophysiological responses, heavy metal accumulation and phytoremoval ability in four willow clones exposed to cadmium under hydroponics. In: Ansari A, Gill S, Gill R, Lanza G, Newman L (Eds.) Phytoremediation: Management of environmental contaminants. Springer Inter Pub, Switzerland pp 87-98.

Jayanthi P, Senthilkumar P, Sivasankar S (2015). Interactive effects of copper and zinc accumulation in Portulaca olearcea stem cuttings, through hydroponics. Advances in Applied Science Research 6(5):54-56.

Jewell W (1994). Resource-recovery wastewater treatment combined anaerobic treatment of primary sewage with hydroponic secondary tertiary treatment. American Scientist 82(4):366-375.

Jones JB Jr (1982). Hydroponics: Its history and use in plant nutrition studies. Journal of Plant Nutrition 5(8):1003-1030.

Klapheck S, Fliegner W, Zimmer I (1994). Hydroxymethylphytochelatins v-glutamylcysteine (N)-serine are metal induced peptides in the Poaceae. Plant Physiology 104(4):1325-1332.

Koleva L, Semerdjieva I, Nikolova A, Vassilev A (2010).Comparative morphological and histological study on zinc- and cadmium-treated durum wheat plants with similar growth inhibition. General and Applied Plant Physiology 36(1-2):8-11.

Kubota H, Takenaka C (2003). Arabis gemmifera is a hyperaccumulator of Cd and Zn. International Journal of Phytoremediation 5(3):197-201.

Kumar A, Prasad MNV (2010). Propagation of Talinum cuneifolium L. (Portulacaceae), an ornamental plant and leafy vegetable, by stem cuttings. Floriculture and Ornamental Biotechnology 4(S1):68-71.

Langille WM, MacLean KS (1976). Some essential nutrient elements in forest plants as related to species, plant part, season and location. Plant and Soil 45(1):17-26.

Lansdown RV (2013). Talinum portulacifolium. Retrieved 2018 August 19 from The IUCN red list of threatened species 2013:e.T13591558A13598358. http://dx.doi.org/10.2305/IUCN. UK.2013-1.RLTS.T13591558A13598 358.en.

Li C, Xiao B, Wang QH, Yao SH, Wu JY (2014). Phytoremediation of Zn- and Cr-contaminated soil using two promising energy grasses. Water Air and Soil Pollution 225(7): 2027, https://doi.org/10.1007/s11270-. 014-2027-5

Lone MI, He Z, Stoffella PJ, Yang X (2008). Phytoremediation of heavy metal polluted soils and water: Progresses and perspective. Journal of Zhejiang University Science B 9(3):210-220.

Makeshkumar K, Thangavel P, Subburam V (1996). Effect of heavy metals on the regeneration potential of the stem cuttings of the medicinal plant Portulaca oleracea Linn. Proceedings of Academy of Environmental Biology 5(2):139-144.

Miller AG, Morris M (2004). Ethnoflora of the Socotra Archipelago. Royal Botanic Garden Edinburgh, Edinburgh.

Mohanapriya S, Senthilkumar P, Sivakumar S, Dineshkumar M, Subbhuraam CV (2006). Effects of copper sulfate and copper nitrate in aquatic medium on the restoration potential and accumulation of copper in stem cuttings of the terrestrial medicinal plant, Portulaca oleracea Linn. Environmental Monitoring and Assessment 121(1-3):233-244.

Nagajyoti PC, Lee KD, Sreekanth TVM (2010). Heavy metals, occurrence and toxicity for plants: a review. Environmental Chemistry Letters 8(3):199-216.

Niu Z, Sun L, Sun T, Li Y, Wang H (2007). Evaluation of phytoextracting cadmium and lead by sunflower, ricinus, alfalfa and mustard in hydroponic culture. JES 19(8):961-967.

Piper CS (1966). Soil and plant analysis. Hans Publishers, Bombay, India.

Plum LM, Rink L, Haase H (2010). The essential toxin: Impact of zinc on human health. International Journal of Environmental Research and Public Health 7(4):1342-1365.

Rajkumar K, Sivakumar S, Senthilkumar P, Prabha D, Subbhuraam CV, Song YC (2009). Effects of selected heavy metals (Pb, Cu, Ni and Cd) in the aquatic medium on the restoration potential and accumulation in the stem cuttings of the terrestrial plant, Talinum triangulare Linn. Ecotoxicology 18(7):952-960.

Rajput KS, Rao KS (2006). Death and decay in the trees of mango (Mangifera indica L.) Microbiological Research162 (3):229-237.

Rout GR, Das P (2003). Effect of metal toxicity on plant growth and metabolism: I. Zinc. In: Lichtfouse E., Navarrete M., Debaeke P., Véronique S., Alberola C. (Eds) Sustainable agriculture. Springer, Dordrecht pp 3-11.

Sadeghzadeh B (2013). A review of zinc nutrition and plant breeding. Journal of Soil Science and Plant Nutrition 13(4):905-927.

Seregin IV, Kozhevnikova AD, Gracheva VV, Bystrova VEI, Ivanov B (2011). Tissue zinc distribution in maize seedling roots and its action on growth. Russian Journal of Plant Physiology 58(1):109-117.

Shahid M, Austruy A, Echevarria G, Arshad M, Sanaullah M, Aslam M, Nadeem M, Nasim W, Dumat C (2014). EDTA-Enhanced phytoremediation of heavy metals: A Review. Soil Sediment and Contamination: An International Journal 23(4):389-416.

Takkar PN, Mann MS (1978). Toxic levels of soil and plant zinc for maize and wheat. Plant and Soil 49(3):667-669.

Tchounwou PB, Yedjou CG, Patlolla AK, Sutton DJ (2012). Heavy metals toxicity and the environment. In: Luch A (Ed.) Molecular, clinical and environmental toxicology. Experientia Supplementum, volume 101. Springer, Basel pp133-164.

Tsonev T, Lidon FJC (2012). Zinc in plants-an overview. Emirates Journal of Food and Agriculture 24(4):322-333.

Vysotskaya LB, Cherkoz’yanova AV, Veselov SY, Kudoyarova GR (2007). Role of auxins and cytokinins in the development of lateral roots in wheat plants with several roots removed. Russian Journal of Plant Physiology 54(3):402-406.

Wang J, Tian C, Zhang C, Shi B, Cao X, Zhang T-Q, Zhao Z, Wang J-W, Jiao Y (2017). Cytokinin signaling activates WUSCHEL expression during axillary meristem initiation. The Plant Cell 29:1373-1387.

Wang W, Wu Y, Akbar S, Jia X, He Z, Tian X (2016). Effect of heavy metals combined stress on growth and metals accumulation of three Salix species with different cutting position. International Journal of Phytoremediation 18(8):761-767.

Wierzbicka M, Obidzinska J (1998). The effect of lead on seed imbibition and germination in different plant species. Plant Science 137(2):155-171.

Wilkins DA (1978). The measurement of tolerance to edaphic factors by means of root growth. New Phytologist 136(3):481-488.

Yanez L, Ortiz D, Calderon J, Batres L, Carrizales L, Mejia J (2002). Overview of human health and chemical mixtures: problems facing developing countries. Environmental Health Perspective 110(S6):901-909.

Zhang P, Sun L, Qin J, Wan J, Wang R, Li S, Xu J (2018). cGMP is involved in Zn tolerance through the modulation of auxin redistribution in root tips. Environment and Experimental Botany 147:22-30.




How to Cite

MUTHUKUMAR, T., JAISON, S., & BABU, S. D. (2018). Zinc Influences Regeneration of Talinum portulacifolium Stem Cuttings in Nutrient Solution. Notulae Scientia Biologicae, 10(4), 530–539. https://doi.org/10.15835/nsb10410392



Research articles
DOI: 10.15835/nsb10410392