Hypoglycaemic and Hypolipidemic Effects of Black Brand of Lipton Tea (Camellia sinensis) on Normal Male Albino Rats


  • E. C. ANORUE University of Nigeria, Department of Zoology and Environmental Biology, Nsukka, Enugu State (NG)
  • E. C. MBEGBU University of Nigeria, Department of Veterinary Physiology and Pharmacology, Nsukka, Enugu State (NG)
  • G. I. NGWU University of Nigeria, Department of Zoology and Environmental Biology, Nsukka, Enugu State (NG)
  • K. N. IBEMENUGA Anambra State University, Department of Biological Sciences, Uli, Anambra State (NG)
  • J. E. EYO University of Nigeria, Department of Zoology and Environmental Biology, Nsukka, Enugu State (NG)




antherogenic index, Camellia sinensis, coronary risk index, hypoglycaemia, rats


Recently, considerable attention has been focused on dietary supplements that possess hypoglycaemic and hypolipidemic properties in lieu of the synergistically synthesized drugs like statins and metiform which have been proven to have adverse effects. This present work was carried out to study the potential effects of Lipton tea aqueous extract (LTAE) on normal male albino rats. 36 six male Wistar rats weighing 115-150 g were assigned into four main groups; each group was divided into three subgroups, consisting of three replicates namely R1, R2 and R3 making a total of nine rats in each of the main groups. Group 1 served as the control and received only water, Group 2 received 200 mg/kg of LTAE, Group 3 received 400 mg/kg of the LTAE, while Group 4 received 800 mg/kg of LTAE. All treatment was given orally on daily basis for 28 consecutive days, but the parameters were assessed on weekly basis. The result revealed that, weight gain, fasting blood sugar and serum (total cholesterol, triglycerides, LDL-c and VLDL-c) significantly (p < 0.05) decrease in all the treated groups while HDL-c significantly (p < 0.05) increased when compared to the control group. The Lipton tea aqueous extract induced both a dose-dependent and a time-dependent effect in the parameters measured, apart from the total cholesterol which did not show a dose-dependent effect. This led to an overall decrease in the antherogenic index and coronary risk index, suggesting that LTAE possesses hypoglycaemic and hypoglycaemic activities in normal male rats.


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Abe K, Ijiri M, Suzuki T, Taguchi K, Koyama Y, Isemura M (2005). Green tea with high catechin content suppresses inflammatory cytokine expression in the galactosamine-injured rat liver. Biomedical Research 26(5):187-192.

Adeneye AA, Olagunju JA (2009). Preliminary hypoglycemic and hypolipidemic activities of the aqueous seed extract of Carica papaya Linn in Wistar rats. Biology and Medicine 1(1):1-10.

Ahmad N, Feyes DK, Agarwal R, Mukhtar H, Nieminen AL (1997). Green tea constituent epigallocatechin-3-gallate and induction of apoptosis and cell cycle arrest in human carcinoma cells. Journal of the National Cancer Institute 89(24):1881-1886.

Ahmed RG (2005). The physiological and biochemical effects of diabetes on the balance between oxidative stress and antioxidant defense system. Medical Journal of Islamic World Academy of Sciences 15(1):31-42.

Aizaki T, Osaka M, Hideyuki HA, Kurokawa S, Matsuyama K, ... Izumi T (1999). Hypokalemia with syncope caused by habitual drinking of oolong tea. Internal Medicine 38(3):252-256.

Al Jamal A (2014). Effect of rosemary (Rosmarinus officinalis) on lipid profiles and blood glucose in human diabetic patients (type-2). African Journal of Biochemistry Research 8(8):147-150.

Allain CC, Poon LS, Chan CS, Richmond WF, Fu PC (1974). Enzymatic determination of total serum cholesterol. Clinical Chemistry 20(4):470-475.

Al-Salafe R, Irshad M, Abdulghani HM (2014). Does green tea help to fight against obesity? An overview of the epidemiological reports. Austin Journal of Clinical Medicine 1(3):1011.

Anderson RA, Polansky MM (2002). Tea enhances insulin activity. Journal of Agricultural and Food Chemistry 50(24):7182-7186.

APA (2012). Guidelines for ethical conduct in the care and use of nonhuman animals in research. USA, Washington DC: American Psychological Association. Retrieved June 13, 2015.

Atiku MK, Ubon GA (2011). Studies on the hypoglycaemic, hypocholesterolaemic and acute toxicity of aqueous Gymnema sylvestre leaf extract in rats. Bio-Research 9(1):738-742.

Bailey CJ, Day C (1989). Traditional plant medicines as treatments for diabetes. Diabetes Care 12(8):553-564.

Baynes JW, Thorpe SR (1999). Role of oxidative stress in diabetic complications: a new perspective on an old paradigm. Diabetes 48:1-9.

Bose M, Lambert JD, Ju J, Reuhl KR, Shapses SA, Yang CS (2008). The major green tea polyphenol, (-) -epigallocatechin-3-gallate, inhibits obesity, metabolic syndrome, and fatty liver disease in high-fat-fed mice. The Journal of Nutrition 38(9):1677-1683.

Broadhurst CL, Polansky MM, Anderson RA (2000). Insulin-like biological activity of culinary and medicinal plant aqueous extracts in vitro. Journal of Agricultural and Food Chemistry 48(3):849-852.

Cameron AR, Anton S, Melville L, Houston NP, Dayal S, ... Rena G (2008). Black tea polyphenols mimic insulin/insulinâ€like growth factorâ€1 signalling to the longevity factor FOXO1a. Aging Cell 7:69-77.

Cao J, Luo SF, Liu JW, Li Y (2004). Safety evaluation on fluoride content in black tea. Food Chemistry 88(2):233-236.

Dulloo AG, Seydoux J, Girardier L, Chantre P, Vandermander J (2000). Green tea and thermogenesis: interactions between catechin-polyphenols, caffeine and sympathetic activity. International Journal of Obesity 24(2):252-258.

Duncan DB (1955). Multiple range and multiple F tests. Biometrics 11(1):1-42.

Eyo JE, Ozougwu JC, Echi PC (2011). Hypoglycaemic effects of Allium cepa, Allium sativum and Zingiber officinale aqueous extracts on alloxan-induced diabetic Rattus novergicus. Medical Journal of the Islamic World Academic Sciences 19(3):121-126.

Ezuruike UF, Prieto JM (2014). The use of plants in the traditional management of diabetes in Nigeria: Pharmacological and toxicological considerations. Journal of Ethnopharmacology 155(2):857-924.

Finley PR, Schifman RB, Williams RJ, Lichti DA (1978). Cholesterol in high-density lipoprotein: use of Mg2+/dextran sulfate in its enzymic measurement. Clinical Chemistry 24(6):931-933.

Fossati P, Prencipe L (1982). Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clinical Chemistry 28(10):2077-2080.

Friedwald WT, Leve RI, Fredrichson DS (1972). Estimation of concentration of low density lipoproteins separated by three different methods. Clinical Chemistry 18(6):499-502.

Harbowy ME, Balentine DA, Davies AP, Cai Y (1997). Tea chemistry. Critical Reviews in Plant Science 16(5):415-480.

Hasanein MA, Gawad HS, El-Megeid AA (2012). Effect of water extract prepared from green tea, black tea and cinnamon on obese rats suffering from diabetes. World Applied Sciences Journal 20(7):976-987.

Ikeda I, Imasato Y, Sasaki E, Nakayama M, Nagao H, ... Sugano M (1992). Tea catechins decrease micellar solubility and intestinal absorption of cholesterol in rats. Biochimica et Biophysica Acta (BBA)-Lipids and Lipid Metabolism 1127(2):141-146.

Khan M, Ali M, Ali A, Mir SR (2014). Hypoglycemic and hypolipidemic activities of Arabic and Indian origin Salvadora persica root extract on diabetic rats with histopathology of their pancreas. International Journal of Health Sciences 8(1):45-56.

Khan N, Mukhtar H (2007). Tea polyphenols for health promotion. Life Sciences 81(7):519-533.

Khan N, Mukhtar H (2013). Tea and health: studies in humans. Current Pharmaceutical Design 19(34):6141-6147.

King H, Aubert RE, Herman WH (1998). Global burden of diabetes, 1995-2025: prevalence, numerical estimates, and projections. Diabetes Care 21(9):1414-1431.

Leung LK, Su Y, Chen R, Zhang Z, Huang Y, Chen ZY (2001). Theaflavins in black tea and catechins in green tea are equally effective antioxidants. The Journal of Nutrition 131(9):2248-2251.

Li S, Lo CY, Pan MH, Lai CS, Ho CT (2013). Black tea: chemical analysis and stability. Food and Function 4(1):10-18.

Lin JK, Lin-Shiau SY (2006). Mechanisms of hypolipidemic and anti-obesity effects of tea and tea polyphenols. Molecular Nutrition and Food Research 50(2):211-217.

Maritim AC, Sanders A, Watkins JB (2003). Diabetes, oxidative stress, and antioxidants: a review. Journal of Biochemical and Molecular Toxicology 17(1):24-38.

Mita M, Borland K, Price JM, Hall PF (1985). The influence of insulin and insulin-like growth factor-I on hexose transport by Sertoli cells. Endocrinology 116(3):987-992.

Murray RK, Granner DK, Mayes PA, Rodwell VW (2014). Harper’s illustrated biochemistry. USA: McGraw-Hill.

Nelson DL, Lehninger AL, Cox MM (2008). Lehninger principles of biochemistry. Macmillan.

Ozougwu JC, Eyo JE (2010). Studies on the anti-diabetic activity of Allium sativum (garlic) aqueous extracts on alloxan-induced diabetic albino rat. Pharmacologyonline 2010(2):1079-1088.

Ozougwu JC, Eyo JE (2011). Evaluation of the activity of Zingiber officinale (ginger) aqueous extracts on alloxan-induced diabetic rats. Pharmacologyonline 2011(1):258-269.

Ozougwu JC, Nwachi UE, Eyo JE (2008). Comparative hypolipidaemic effects of Allium cepa, Allium sativum and Zingiber officinale aqueous extracts on alloxan-induced diabetic Rattus novergicus. Bio-Research 6(2):384-391.

Parasuraman S, Raveendran R, Kesavan R (2010). Blood sample collection in small laboratory animals. Journal of Pharmacology and Pharmacotherapeutics 1(2):87-93.

Prasad SK, Kulshreshtha A, Qureshi TN (2009). Antidiabetic activity of some herbal plants in streptozotocin induced diabetic albino rats. Pakistan Journal of Nutrition 8(5):551-557.

Richmond N (1973). Colorimetric determination of total cholesterol and high density lipoprotein cholesterol (HDL-c). Clinical Chemistry 19:1350-1356.

Trinder P (1969). Determination of blood glucose using an oxidase-peroxidase system with a non-carcinogenic chromogen. Journal of Clinical Pathology 22(2):158-161.

Wang H, Wen Y, Du Y, Yan X, Guo H, Rycroft JA, ... Mela DJ 2010). Effects of catechin enriched green tea on body composition. Obesity 18(4):773-779.

Wang S, Noh SK, Koo SI (2006). Epigallocatechin gallate and caffeine differentially inhibit the intestinal absorption of cholesterol and fat in ovariectomized rats. Journal of Nutrition 136(11):2791-2796.

Wickelgren I (1998). Obesity: How big a problem? Science 280(5368):1364-1367.

Willett W, Manson J, Liu S (2002). Glycemic index, glycemic load, and risk of type 2 diabetes. American Journal of Clinical Nutrition 76(1):274S-280S.

Yashin A, Yashin Y, Nemzer B (2013). Beneficial effect of tea on human health. American Journal of Biomedical Sciences 5(4):226-241.




How to Cite

ANORUE, E. C., MBEGBU, E. C., NGWU, G. I., IBEMENUGA, K. N., & EYO, J. E. (2019). Hypoglycaemic and Hypolipidemic Effects of Black Brand of Lipton Tea (Camellia sinensis) on Normal Male Albino Rats. Notulae Scientia Biologicae, 11(1), 94–101. https://doi.org/10.15835/nsb11110370



Research articles
DOI: 10.15835/nsb11110370